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Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles

Author

Listed:
  • Conghui Tian

    (University of Science and Technology of China)

  • Lingxiao Shen

    (University of Science and Technology of China)

  • Chenjia Gong

    (University of Science and Technology of China)

  • Yunxia Cao

    (The First Affiliated Hospital of Anhui Medical University
    Anhui Provincial Engineering Research Center of Biopreservation and Artificial Organs)

  • Qinghua Shi

    (University of Science and Technology of China)

  • Gang Zhao

    (University of Science and Technology of China)

Abstract

Preantral follicles are often used as models for cryopreservation and in vitro culture due to their easy availability. As a promising approach for mammalian fertility preservation, vitrification of preantral follicles requires high concentrations of highly toxic penetrating cryoprotective agents (up to 6 M). Here, we accomplish low-concentration-penetrating cryoprotective agent (1.5 M) vitrification of mouse preantral follicles encapsulated in hydrogel by nanowarming. We find that compared with conventional water bath warming, the viability of preantral follicles is increased by 33%. Moreover, the cavity formation rate of preantral follicles after in vitro culture is comparable to the control group without vitrification. Furthermore, the percentage of MII oocytes developed from the vitrified follicles, and the birth rate of offspring following in vitro fertilization and embryo transfer are also similar to the control group. Our results provide a step towards nontoxic vitrification by utilizing the synergistic cryoprotection effect of microencapsulation and nanowarming.

Suggested Citation

  • Conghui Tian & Lingxiao Shen & Chenjia Gong & Yunxia Cao & Qinghua Shi & Gang Zhao, 2022. "Microencapsulation and nanowarming enables vitrification cryopreservation of mouse preantral follicles," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-34549-2
    DOI: 10.1038/s41467-022-34549-2
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    References listed on IDEAS

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    1. Monica M. Laronda & Alexandra L. Rutz & Shuo Xiao & Kelly A. Whelan & Francesca E. Duncan & Eric W. Roth & Teresa K. Woodruff & Ramille N. Shah, 2017. "A bioprosthetic ovary created using 3D printed microporous scaffolds restores ovarian function in sterilized mice," Nature Communications, Nature, vol. 8(1), pages 1-10, August.
    2. Fabienne Bachtiger & Thomas R. Congdon & Christopher Stubbs & Matthew I. Gibson & Gabriele C. Sosso, 2021. "The atomistic details of the ice recrystallisation inhibition activity of PVA," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    3. Guoying Bai & Dong Gao & Zhang Liu & Xin Zhou & Jianjun Wang, 2019. "Probing the critical nucleus size for ice formation with graphene oxide nanosheets," Nature, Nature, vol. 576(7787), pages 437-441, December.
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