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RNA export through the nuclear pore complex is directional

Author

Listed:
  • Asaf Ashkenazy-Titelman

    (Bar-Ilan University)

  • Mohammad Khaled Atrash

    (Bar-Ilan University)

  • Alon Boocholez

    (Bar-Ilan University)

  • Noa Kinor

    (Bar-Ilan University)

  • Yaron Shav-Tal

    (Bar-Ilan University)

Abstract

The changes occurring in mRNA organization during nucleo-cytoplasmic transport and export, are not well understood. Moreover, directionality of mRNA passage through the nuclear pore complex (NPC) has not been examined within individual NPCs. Here we find that an mRNP is compact during nucleoplasmic travels compared to a more open structure after transcription and at the nuclear periphery. Compaction levels of nuclear transcripts can be modulated by varying levels of SR proteins and by changing genome organization. Nuclear mRNPs are mostly rod-shaped with distant 5'/3'-ends, although for some, the ends are in proximity. The latter is more abundant in the cytoplasm and can be modified by translation inhibition. mRNAs and lncRNAs exiting the NPC exhibit predominant 5’-first export. In some cases, several adjacent NPCs are engaged in export of the same mRNA suggesting 'gene gating'. Altogether, we show that the mRNP is a flexible structure during travels, with 5'-directionality during export.

Suggested Citation

  • Asaf Ashkenazy-Titelman & Mohammad Khaled Atrash & Alon Boocholez & Noa Kinor & Yaron Shav-Tal, 2022. "RNA export through the nuclear pore complex is directional," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33572-7
    DOI: 10.1038/s41467-022-33572-7
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    References listed on IDEAS

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    1. I. Bronshtein & E. Kepten & I. Kanter & S. Berezin & M. Lindner & Abena B. Redwood & S Mai & S. Gonzalo & R. Foisner & Y. Shav-Tal & Y. Garini, 2015. "Loss of lamin A function increases chromatin dynamics in the nuclear interior," Nature Communications, Nature, vol. 6(1), pages 1-9, November.
    2. Anthony P. Schuller & Matthias Wojtynek & David Mankus & Meltem Tatli & Rafael Kronenberg-Tenga & Saroj G. Regmi & Phat V. Dip & Abigail K. R. Lytton-Jean & Edward J. Brignole & Mary Dasso & Karsten W, 2021. "The cellular environment shapes the nuclear pore complex architecture," Nature, Nature, vol. 598(7882), pages 667-671, October.
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