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Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target

Author

Listed:
  • Heewon Cho

    (Pohang University of Science and Technology (POSTECH))

  • Haw-Young Kwon

    (Institute for Basic Science (IBS))

  • Amit Sharma

    (Pohang University of Science and Technology (POSTECH))

  • Sun Hyeok Lee

    (Pohang University of Science and Technology (POSTECH))

  • Xiao Liu

    (Pohang University of Science and Technology (POSTECH))

  • Naoki Miyamoto

    (Institute for Basic Science (IBS))

  • Jong-Jin Kim

    (Sunchon National University)

  • Sin-Hyeog Im

    (Pohang University of Science and Technology (POSTECH)
    ImmunoBiome Inc.
    Yonsei University)

  • Nam-Young Kang

    (Pohang University of Science and Technology (POSTECH))

  • Young-Tae Chang

    (Pohang University of Science and Technology (POSTECH)
    Institute for Basic Science (IBS)
    Pohang University of Science and Technology (POSTECH))

Abstract

Macrophages play crucial roles in protecting our bodies from infection and cancers. As macrophages are multi-functional immune cells, they have diverse plastic subsets, such as M1 and M2, derived from naïve M0 cells. Subset-specific macrophage probes are essential for deciphering and monitoring the various activation of macrophages, but developing such probes has been challenging. Here we report a fluorescent probe, CDr17, which is selective for M1 macrophages over M2 or M0. The selective staining mechanism of CDr17 is explicated as Gating-Oriented Live-cell Distinction (GOLD) through overexpressed GLUT1 in M1 macrophages. Finally, we demonstrate the suitability of CDr17 to track M1 macrophages in vivo in a rheumatoid arthritis animal model.

Suggested Citation

  • Heewon Cho & Haw-Young Kwon & Amit Sharma & Sun Hyeok Lee & Xiao Liu & Naoki Miyamoto & Jong-Jin Kim & Sin-Hyeog Im & Nam-Young Kang & Young-Tae Chang, 2022. "Visualizing inflammation with an M1 macrophage selective probe via GLUT1 as the gating target," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-33526-z
    DOI: 10.1038/s41467-022-33526-z
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    References listed on IDEAS

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    1. Thomas A. Wynn & Ajay Chawla & Jeffrey W. Pollard, 2013. "Macrophage biology in development, homeostasis and disease," Nature, Nature, vol. 496(7446), pages 445-455, April.
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