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Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli

Author

Listed:
  • Kevin W. Zhu

    (Duke University School of Medicine)

  • Shawn D. Burton

    (Lehigh University
    University of Utah School of Medicine)

  • Maira H. Nagai

    (Duke University School of Medicine)

  • Justin D. Silverman

    (Pennsylvania State University
    Pennsylvania State University
    Pennsylvania State University
    Pennsylvania State University)

  • Claire A. March

    (Duke University School of Medicine)

  • Matt Wachowiak

    (University of Utah School of Medicine)

  • Hiroaki Matsunami

    (Duke University School of Medicine
    Duke University School of Medicine
    Duke University)

Abstract

Sensory processing in olfactory systems is organized across olfactory bulb glomeruli, wherein axons of peripheral sensory neurons expressing the same olfactory receptor co-terminate to transmit receptor-specific activity to central neurons. Understanding how receptors map to glomeruli is therefore critical to understanding olfaction. High-throughput spatial transcriptomics is a rapidly advancing field, but low-abundance olfactory receptor expression within glomeruli has previously precluded high-throughput mapping of receptors to glomeruli in the mouse. Here we combined sequential sectioning along the anteroposterior, dorsoventral, and mediolateral axes with target capture enrichment sequencing to overcome low-abundance target expression. This strategy allowed us to spatially map 86% of olfactory receptors across the olfactory bulb and uncover a relationship between OR sequence and glomerular position.

Suggested Citation

  • Kevin W. Zhu & Shawn D. Burton & Maira H. Nagai & Justin D. Silverman & Claire A. March & Matt Wachowiak & Hiroaki Matsunami, 2022. "Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-32267-3
    DOI: 10.1038/s41467-022-32267-3
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    References listed on IDEAS

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    1. Mayer Claus-Dieter & Lorent Julie & Horgan Graham W, 2011. "Exploratory Analysis of Multiple Omics Datasets Using the Adjusted RV Coefficient," Statistical Applications in Genetics and Molecular Biology, De Gruyter, vol. 10(1), pages 1-27, March.
    2. Harumi Saito & Hirofumi Nishizumi & Satoshi Suzuki & Hideyuki Matsumoto & Nao Ieki & Takaya Abe & Hiroshi Kiyonari & Masahiko Morita & Hideo Yokota & Nozomi Hirayama & Takahiro Yamazaki & Takefumi Kik, 2017. "Immobility responses are induced by photoactivation of single glomerular species responsive to fox odour TMT," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    3. Jinsong Li & Tomohiro Ishii & Paul Feinstein & Peter Mombaerts, 2004. "Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons," Nature, Nature, vol. 428(6981), pages 393-399, March.
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