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Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons

Author

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  • Jinsong Li

    (The Rockefeller University)

  • Tomohiro Ishii

    (The Rockefeller University)

  • Paul Feinstein

    (The Rockefeller University)

  • Peter Mombaerts

    (The Rockefeller University)

Abstract

Of the ∼1,000 odorant receptor (OR) genes in the mouse genome, an olfactory sensory neuron (OSN) is thought to express one gene, from one allele. This is reminiscent of immunoglobulin and T-cell receptor genes, which undergo DNA rearrangements in lymphocytes. Here, we test the hypothesis that OR gene choice is controlled by DNA rearrangements in OSNs. Using permanent genetic marking, we show that the choice by an OSN to express an allele of the OR gene M71 is irreversible. Using M71-expressing OSNs as donors for nuclear transfer, we generate blastocysts, embryonic stem (ntES) cell lines and clonal mice. DNA analysis of these cell lines, whose genome is clonally derived from an M71-expressing OSN, does not reveal DNA rearrangements or sequence alterations at the M71 locus. OSNs that differentiate from ntES cells after injection into blastocysts are not restricted to expression of M71 but can express other OR genes. Thus, M71 gene choice is irreversible but is reset upon nuclear transfer, and is not accompanied by genomic alterations.

Suggested Citation

  • Jinsong Li & Tomohiro Ishii & Paul Feinstein & Peter Mombaerts, 2004. "Odorant receptor gene choice is reset by nuclear transfer from mouse olfactory sensory neurons," Nature, Nature, vol. 428(6981), pages 393-399, March.
    • Handle: RePEc:nat:nature:v:428:y:2004:i:6981:d:10.1038_nature02433
    DOI: 10.1038/nature02433
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    Cited by:

    1. Jane S. Huang & Tenzin Kunkhyen & Alexander N. Rangel & Taryn R. Brechbill & Jordan D. Gregory & Emily D. Winson-Bushby & Beichen Liu & Jonathan T. Avon & Ryan J. Muggleton & Claire E. J. Cheetham, 2022. "Immature olfactory sensory neurons provide behaviourally relevant sensory input to the olfactory bulb," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    2. Kevin W. Zhu & Shawn D. Burton & Maira H. Nagai & Justin D. Silverman & Claire A. March & Matt Wachowiak & Hiroaki Matsunami, 2022. "Decoding the olfactory map through targeted transcriptomics links murine olfactory receptors to glomeruli," Nature Communications, Nature, vol. 13(1), pages 1-18, December.

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