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Intestine-enriched apolipoprotein b orthologs are required for stem cell progeny differentiation and regeneration in planarians

Author

Listed:
  • Lily L. Wong

    (Oklahoma Medical Research Foundation)

  • Christina G. Bruxvoort

    (Oklahoma Medical Research Foundation
    Oklahoma Medical Research Foundation
    University of Oklahoma Health Sciences Center
    Department of Veteran Affairs Medical Center - Research Services)

  • Nicholas I. Cejda

    (Oklahoma Medical Research Foundation
    Oklahoma Medical Research Foundation)

  • Matthew R. Delaney

    (Oklahoma Medical Research Foundation)

  • Jannette Rodriguez Otero

    (University of Illinois at Urbana-Champaign
    Universidad Interamericana de Puerto Rico)

  • David J. Forsthoefel

    (Oklahoma Medical Research Foundation
    University of Oklahoma Health Sciences Center)

Abstract

Lipid metabolism plays an instructive role in regulating stem cell state and differentiation. However, the roles of lipid mobilization and utilization in stem cell-driven regeneration are unclear. Planarian flatworms readily restore missing tissue due to injury-induced activation of pluripotent somatic stem cells called neoblasts. Here, we identify two intestine-enriched orthologs of apolipoprotein b, apob-1 and apob-2, which mediate transport of neutral lipid stores from the intestine to target tissues including neoblasts, and are required for tissue homeostasis and regeneration. Inhibition of apob function by RNAi causes head regression and lysis in uninjured animals, and delays body axis re-establishment and regeneration of multiple organs in amputated fragments. Furthermore, apob RNAi causes expansion of the population of differentiating neoblast progeny and dysregulates expression of genes enriched in differentiating and mature cells in eight major cell type lineages. We conclude that intestine-derived lipids serve as a source of metabolites required for neoblast progeny differentiation.

Suggested Citation

  • Lily L. Wong & Christina G. Bruxvoort & Nicholas I. Cejda & Matthew R. Delaney & Jannette Rodriguez Otero & David J. Forsthoefel, 2022. "Intestine-enriched apolipoprotein b orthologs are required for stem cell progeny differentiation and regeneration in planarians," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31385-2
    DOI: 10.1038/s41467-022-31385-2
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    Cited by:

    1. Ryan E. Hulett & Julian O. Kimura & D. Marcela Bolaños & Yi-Jyun Luo & Carlos Rivera-López & Lorenzo Ricci & Mansi Srivastava, 2023. "Acoel single-cell atlas reveals expression dynamics and heterogeneity of adult pluripotent stem cells," Nature Communications, Nature, vol. 14(1), pages 1-15, December.

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