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Intestine-enriched apolipoprotein b orthologs are required for stem cell progeny differentiation and regeneration in planarians

Author

Listed:
  • Lily L. Wong

    (Oklahoma Medical Research Foundation)

  • Christina G. Bruxvoort

    (Oklahoma Medical Research Foundation
    Oklahoma Medical Research Foundation
    University of Oklahoma Health Sciences Center
    Department of Veteran Affairs Medical Center - Research Services)

  • Nicholas I. Cejda

    (Oklahoma Medical Research Foundation
    Oklahoma Medical Research Foundation)

  • Matthew R. Delaney

    (Oklahoma Medical Research Foundation)

  • Jannette Rodriguez Otero

    (University of Illinois at Urbana-Champaign
    Universidad Interamericana de Puerto Rico)

  • David J. Forsthoefel

    (Oklahoma Medical Research Foundation
    University of Oklahoma Health Sciences Center)

Abstract

Lipid metabolism plays an instructive role in regulating stem cell state and differentiation. However, the roles of lipid mobilization and utilization in stem cell-driven regeneration are unclear. Planarian flatworms readily restore missing tissue due to injury-induced activation of pluripotent somatic stem cells called neoblasts. Here, we identify two intestine-enriched orthologs of apolipoprotein b, apob-1 and apob-2, which mediate transport of neutral lipid stores from the intestine to target tissues including neoblasts, and are required for tissue homeostasis and regeneration. Inhibition of apob function by RNAi causes head regression and lysis in uninjured animals, and delays body axis re-establishment and regeneration of multiple organs in amputated fragments. Furthermore, apob RNAi causes expansion of the population of differentiating neoblast progeny and dysregulates expression of genes enriched in differentiating and mature cells in eight major cell type lineages. We conclude that intestine-derived lipids serve as a source of metabolites required for neoblast progeny differentiation.

Suggested Citation

  • Lily L. Wong & Christina G. Bruxvoort & Nicholas I. Cejda & Matthew R. Delaney & Jannette Rodriguez Otero & David J. Forsthoefel, 2022. "Intestine-enriched apolipoprotein b orthologs are required for stem cell progeny differentiation and regeneration in planarians," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-31385-2
    DOI: 10.1038/s41467-022-31385-2
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    1. Yu-ichi Tsukada & Jia Fang & Hediye Erdjument-Bromage & Maria E. Warren & Christoph H. Borchers & Paul Tempst & Yi Zhang, 2006. "Histone demethylation by a family of JmjC domain-containing proteins," Nature, Nature, vol. 439(7078), pages 811-816, February.
    2. Marlen Knobloch & Simon M. G. Braun & Luis Zurkirchen & Carolin von Schoultz & Nicola Zamboni & Marcos J. Araúzo-Bravo & Werner J. Kovacs & Özlem Karalay & Ueli Suter & Raquel A. C. Machado & Marta Ro, 2013. "Metabolic control of adult neural stem cell activity by Fasn-dependent lipogenesis," Nature, Nature, vol. 493(7431), pages 226-230, January.
    3. Lauren E. Cote & Eric Simental & Peter W. Reddien, 2019. "Muscle functions as a connective tissue and source of extracellular matrix in planarians," Nature Communications, Nature, vol. 10(1), pages 1-13, December.
    4. Shree Ram Singh & Xiankun Zeng & Jiangsha Zhao & Ying Liu & Gerald Hou & Hanhan Liu & Steven X. Hou, 2016. "The lipolysis pathway sustains normal and transformed stem cells in adult Drosophila," Nature, Nature, vol. 538(7623), pages 109-113, October.
    5. Yuliana Mihaylova & Prasad Abnave & Damian Kao & Samantha Hughes & Alvina Lai & Farah Jaber-Hijazi & Nobuyoshi Kosaka & A. Aziz Aboobaker, 2018. "Conservation of epigenetic regulation by the MLL3/4 tumour suppressor in planarian pluripotent stem cells," Nature Communications, Nature, vol. 9(1), pages 1-17, December.
    6. Daniela Panáková & Hein Sprong & Eric Marois & Christoph Thiele & Suzanne Eaton, 2005. "Lipoprotein particles are required for Hedgehog and Wingless signalling," Nature, Nature, vol. 435(7038), pages 58-65, May.
    7. Junsu Kang & Jianxin Hu & Ravi Karra & Amy L. Dickson & Valerie A. Tornini & Gregory Nachtrab & Matthew Gemberling & Joseph A. Goldman & Brian L. Black & Kenneth D. Poss, 2016. "Modulation of tissue repair by regeneration enhancer elements," Nature, Nature, vol. 532(7598), pages 201-206, April.
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