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Taf2 mediates DNA binding of Taf14

Author

Listed:
  • Brianna J. Klein

    (University of Colorado School of Medicine)

  • Jordan T. Feigerle

    (Stanford University
    Vanderbilt University School of Medicine)

  • Jibo Zhang

    (The University of North Carolina School of Medicine)

  • Christopher C. Ebmeier

    (University of Colorado)

  • Lixin Fan

    (SAXS Core Facility of the National Cancer Institute)

  • Rohit K. Singh

    (University of Colorado School of Medicine)

  • Wesley W. Wang

    (Texas A&M University)

  • Lauren R. Schmitt

    (University of Colorado School of Medicine)

  • Thomas Lee

    (SAXS Core Facility of the National Cancer Institute)

  • Kirk C. Hansen

    (University of Colorado School of Medicine)

  • Wenshe R. Liu

    (Texas A&M University)

  • Yun-Xing Wang

    (National Institutes of Health)

  • Brian D. Strahl

    (The University of North Carolina School of Medicine)

  • P. Anthony Weil

    (Vanderbilt University School of Medicine)

  • Tatiana G. Kutateladze

    (University of Colorado School of Medicine)

Abstract

The assembly and function of the yeast general transcription factor TFIID complex requires specific contacts between its Taf14 and Taf2 subunits, however, the mechanism underlying these contacts remains unclear. Here, we determined the molecular and structural basis by which the YEATS and ET domains of Taf14 bind to the C-terminal tail of Taf2 and identified a unique DNA-binding activity of the linker region connecting the two domains. We show that in the absence of ligands the linker region of Taf14 is occluded by the surrounding domains, and therefore the DNA binding function of Taf14 is autoinhibited. Binding of Taf2 promotes a conformational rearrangement in Taf14, resulting in a release of the linker for the engagement with DNA and the nucleosome. Genetic in vivo data indicate that the association of Taf14 with both Taf2 and DNA is essential for transcriptional regulation. Our findings provide a basis for deciphering the role of individual TFIID subunits in mediating gene transcription.

Suggested Citation

  • Brianna J. Klein & Jordan T. Feigerle & Jibo Zhang & Christopher C. Ebmeier & Lixin Fan & Rohit K. Singh & Wesley W. Wang & Lauren R. Schmitt & Thomas Lee & Kirk C. Hansen & Wenshe R. Liu & Yun-Xing W, 2022. "Taf2 mediates DNA binding of Taf14," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30937-w
    DOI: 10.1038/s41467-022-30937-w
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    References listed on IDEAS

    as
    1. Ho Sung Rhee & B. Franklin Pugh, 2012. "Erratum: Genome-wide structure and organization of eukaryotic pre-initiation complexes," Nature, Nature, vol. 487(7405), pages 128-128, July.
    2. Jovylyn Gatchalian & Xiaodong Wang & Jinzen Ikebe & Khan L. Cox & Adam H. Tencer & Yi Zhang & Nathaniel L. Burge & Luo Di & Matthew D. Gibson & Catherine A. Musselman & Michael G. Poirier & Hidetoshi , 2017. "Accessibility of the histone H3 tail in the nucleosome for binding of paired readers," Nature Communications, Nature, vol. 8(1), pages 1-10, December.
    3. Ho Sung Rhee & B. Franklin Pugh, 2012. "Genome-wide structure and organization of eukaryotic pre-initiation complexes," Nature, Nature, vol. 483(7389), pages 295-301, March.
    4. Robert K. Louder & Yuan He & José Ramón López-Blanco & Jie Fang & Pablo Chacón & Eva Nogales, 2016. "Correction: Corrigendum: Structure of promoter-bound TFIID and model of human pre-initiation complex assembly," Nature, Nature, vol. 536(7614), pages 112-112, August.
    5. Olga Kolesnikova & Adam Ben-Shem & Jie Luo & Jeff Ranish & Patrick Schultz & Gabor Papai, 2018. "Molecular structure of promoter-bound yeast TFIID," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    6. Robert K. Louder & Yuan He & José Ramón López-Blanco & Jie Fang & Pablo Chacón & Eva Nogales, 2016. "Structure of promoter-bound TFIID and model of human pre-initiation complex assembly," Nature, Nature, vol. 531(7596), pages 604-609, March.
    7. Guochao Chen & Duo Wang & Bin Wu & Fuxiang Yan & Hongjuan Xue & Quanmeng Wang & Shu Quan & Yong Chen, 2020. "Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation," Nature Communications, Nature, vol. 11(1), pages 1-14, December.
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    Cited by:

    1. Minh Chau Nguyen & Hosein Rostamian & Ana Raman & Pengcheng Wei & Dustin C. Becht & Annette H. Erbse & Brianna J. Klein & Tonya M. Gilbert & Gongyi Zhang & M. Andres Blanco & Brian D. Strahl & Sean D., 2024. "Molecular insight into interactions between the Taf14, Yng1 and Sas3 subunits of the NuA3 complex," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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