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Structure of promoter-bound TFIID and model of human pre-initiation complex assembly

Author

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  • Robert K. Louder

    (Biophysics Graduate Group, University of California, Berkeley)

  • Yuan He

    (QB3 Institute, University of California
    Lawrence Berkeley National Laboratory
    †Present address: Interdisciplinary Biological Sciences Program, Northwestern University, Evanston, Illinois 60208, USA.)

  • José Ramón López-Blanco

    (Rocasolano Physical Chemistry Institute)

  • Jie Fang

    (Howard Hughes Medical Institute, University of California)

  • Pablo Chacón

    (Rocasolano Physical Chemistry Institute)

  • Eva Nogales

    (QB3 Institute, University of California
    Lawrence Berkeley National Laboratory
    Howard Hughes Medical Institute, University of California)

Abstract

The general transcription factor IID (TFIID) plays a central role in the initiation of RNA polymerase II (Pol II)-dependent transcription by nucleating pre-initiation complex (PIC) assembly at the core promoter. TFIID comprises the TATA-binding protein (TBP) and 13 TBP-associated factors (TAF1–13), which specifically interact with a variety of core promoter DNA sequences. Here we present the structure of human TFIID in complex with TFIIA and core promoter DNA, determined by single-particle cryo-electron microscopy at sub-nanometre resolution. All core promoter elements are contacted by subunits of TFIID, with TAF1 and TAF2 mediating major interactions with the downstream promoter. TFIIA bridges the TBP–TATA complex with lobe B of TFIID. We also present the cryo-electron microscopy reconstruction of a fully assembled human TAF-less PIC. Superposition of common elements between the two structures provides novel insights into the general role of TFIID in promoter recognition, PIC assembly, and transcription initiation.

Suggested Citation

  • Robert K. Louder & Yuan He & José Ramón López-Blanco & Jie Fang & Pablo Chacón & Eva Nogales, 2016. "Structure of promoter-bound TFIID and model of human pre-initiation complex assembly," Nature, Nature, vol. 531(7596), pages 604-609, March.
    • Handle: RePEc:nat:nature:v:531:y:2016:i:7596:d:10.1038_nature17394
    DOI: 10.1038/nature17394
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    Cited by:

    1. Jay C Brown, 2018. "Control of human gene expression: High abundance of divergent transcription in genes containing both INR and BRE elements in the core promoter," PLOS ONE, Public Library of Science, vol. 13(8), pages 1-19, August.
    2. Brianna J. Klein & Jordan T. Feigerle & Jibo Zhang & Christopher C. Ebmeier & Lixin Fan & Rohit K. Singh & Wesley W. Wang & Lauren R. Schmitt & Thomas Lee & Kirk C. Hansen & Wenshe R. Liu & Yun-Xing W, 2022. "Taf2 mediates DNA binding of Taf14," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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