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Molecular structure of promoter-bound yeast TFIID

Author

Listed:
  • Olga Kolesnikova

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique, UMR7104
    Institut National de la Santé et de la Recherche Médicale, U1258
    Université de Strasbourg)

  • Adam Ben-Shem

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique, UMR7104
    Institut National de la Santé et de la Recherche Médicale, U1258
    Université de Strasbourg)

  • Jie Luo

    (Institute for Systems Biology)

  • Jeff Ranish

    (Institute for Systems Biology)

  • Patrick Schultz

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique, UMR7104
    Institut National de la Santé et de la Recherche Médicale, U1258
    Université de Strasbourg)

  • Gabor Papai

    (Institut de Génétique et de Biologie Moléculaire et Cellulaire
    Centre National de la Recherche Scientifique, UMR7104
    Institut National de la Santé et de la Recherche Médicale, U1258
    Université de Strasbourg)

Abstract

Transcription preinitiation complex assembly on the promoters of protein encoding genes is nucleated in vivo by TFIID composed of the TATA-box Binding Protein (TBP) and 13 TBP-associate factors (Tafs) providing regulatory and chromatin binding functions. Here we present the cryo-electron microscopy structure of promoter-bound yeast TFIID at a resolution better than 5 Å, except for a flexible domain. We position the crystal structures of several subunits and, in combination with cross-linking studies, describe the quaternary organization of TFIID. The compact tri lobed architecture is stabilized by a topologically closed Taf5-Taf6 tetramer. We confirm the unique subunit stoichiometry prevailing in TFIID and uncover a hexameric arrangement of Tafs containing a histone fold domain in the Twin lobe.

Suggested Citation

  • Olga Kolesnikova & Adam Ben-Shem & Jie Luo & Jeff Ranish & Patrick Schultz & Gabor Papai, 2018. "Molecular structure of promoter-bound yeast TFIID," Nature Communications, Nature, vol. 9(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:9:y:2018:i:1:d:10.1038_s41467-018-07096-y
    DOI: 10.1038/s41467-018-07096-y
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    Cited by:

    1. Brianna J. Klein & Jordan T. Feigerle & Jibo Zhang & Christopher C. Ebmeier & Lixin Fan & Rohit K. Singh & Wesley W. Wang & Lauren R. Schmitt & Thomas Lee & Kirk C. Hansen & Wenshe R. Liu & Yun-Xing W, 2022. "Taf2 mediates DNA binding of Taf14," Nature Communications, Nature, vol. 13(1), pages 1-11, December.

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