IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v13y2022i1d10.1038_s41467-022-30431-3.html
   My bibliography  Save this article

Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS

Author

Listed:
  • Peng Shi

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Xiaoyu Ren

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Jie Meng

    (Tsinghua University)

  • Chenlu Kang

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Yihe Wu

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Yingxue Rong

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Shujuan Zhao

    (Peking University)

  • Zhaodi Jiang

    (National Institute of Biological Sciences, Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University)

  • Ling Liang

    (Peking University Health Science Center)

  • Wanzhong He

    (National Institute of Biological Sciences, Tsinghua Institute of Multidisciplinary Biomedical Research, Tsinghua University)

  • Yuxin Yin

    (School of Basic Medical Sciences, Peking University Health Science Center)

  • Xiangdong Li

    (Chinese Academy of Sciences)

  • Yong Liu

    (Xuzhou Medical University)

  • Xiaoshuai Huang

    (Peking University)

  • Yujie Sun

    (Peking University)

  • Bo Li

    (Tsinghua University)

  • Congying Wu

    (School of Basic Medical Sciences, Peking University Health Science Center
    Peking University)

Abstract

The folded mitochondria inner membrane-cristae is the structural foundation for oxidative phosphorylation (OXPHOS) and energy production. By mechanically simulating mitochondria morphogenesis, we speculate that efficient sculpting of the cristae is organelle non-autonomous. It has long been inferred that folding requires buckling in living systems. However, the tethering force for cristae formation and regulation has not been identified. Combining electron tomography, proteomics strategies, super resolution live cell imaging and mathematical modeling, we reveal that the mitochondria localized actin motor-myosin 19 (Myo19) is critical for maintaining cristae structure, by associating with the SAM-MICOS super complex. We discover that depletion of Myo19 or disruption of its motor activity leads to altered mitochondria membrane potential and decreased OXPHOS. We propose that Myo19 may act as a mechanical tether for effective ridging of the mitochondria cristae, thus sustaining the energy homeostasis essential for various cellular functions.

Suggested Citation

  • Peng Shi & Xiaoyu Ren & Jie Meng & Chenlu Kang & Yihe Wu & Yingxue Rong & Shujuan Zhao & Zhaodi Jiang & Ling Liang & Wanzhong He & Yuxin Yin & Xiangdong Li & Yong Liu & Xiaoshuai Huang & Yujie Sun & B, 2022. "Mechanical instability generated by Myosin 19 contributes to mitochondria cristae architecture and OXPHOS," Nature Communications, Nature, vol. 13(1), pages 1-14, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30431-3
    DOI: 10.1038/s41467-022-30431-3
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-022-30431-3
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-022-30431-3?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Hironori Takeda & Akihisa Tsutsumi & Tomohiro Nishizawa & Caroline Lindau & Jon V. Busto & Lena-Sophie Wenz & Lars Ellenrieder & Kenichiro Imai & Sebastian P. Straub & Waltraut Mossmann & Jian Qiu & Y, 2021. "Mitochondrial sorting and assembly machinery operates by β-barrel switching," Nature, Nature, vol. 590(7844), pages 163-169, February.
    2. Andrew S. Moore & Stephen M. Coscia & Cory L. Simpson & Fabian E. Ortega & Eric C. Wait & John M. Heddleston & Jeffrey J. Nirschl & Christopher J. Obara & Pedro Guedes-Dias & C. Alexander Boecker & Te, 2021. "Actin cables and comet tails organize mitochondrial networks in mitosis," Nature, Nature, vol. 591(7851), pages 659-664, March.
    3. Hawa-Racine Thiam & Pablo Vargas & Nicolas Carpi & Carolina Lage Crespo & Matthew Raab & Emmanuel Terriac & Megan C. King & Jordan Jacobelli & Arthur S. Alberts & Theresia Stradal & Ana-Maria Lennon-D, 2016. "Perinuclear Arp2/3-driven actin polymerization enables nuclear deformation to facilitate cell migration through complex environments," Nature Communications, Nature, vol. 7(1), pages 1-14, April.
    4. Souvik Modi & Guillermo López-Doménech & Elise F. Halff & Christian Covill-Cooke & Davor Ivankovic & Daniela Melandri & I. Lorena Arancibia-Cárcamo & Jemima J. Burden & Alan R. Lowe & Josef T. Kittler, 2019. "Miro clusters regulate ER-mitochondria contact sites and link cristae organization to the mitochondrial transport machinery," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Shuo-Shuo Liu & Tian-Xia Jiang & Fan Bu & Ji-Lan Zhao & Guang-Fei Wang & Guo-Heng Yang & Jie-Yan Kong & Yun-Fan Qie & Pei Wen & Li-Bin Fan & Ning-Ning Li & Ning Gao & Xiao-Bo Qiu, 2024. "Molecular mechanisms underlying the BIRC6-mediated regulation of apoptosis and autophagy," Nature Communications, Nature, vol. 15(1), pages 1-16, December.
    2. Tomas Venit & Oscar Sapkota & Wael Said Abdrabou & Palanikumar Loganathan & Renu Pasricha & Syed Raza Mahmood & Nadine Hosny El Said & Shimaa Sherif & Sneha Thomas & Salah Abdelrazig & Shady Amin & Da, 2023. "Positive regulation of oxidative phosphorylation by nuclear myosin 1 protects cells from metabolic reprogramming and tumorigenesis in mice," Nature Communications, Nature, vol. 14(1), pages 1-24, December.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Jingjing Zhao & Xiaojun Han, 2024. "Investigation of artificial cells containing the Par system for bacterial plasmid segregation and inheritance mimicry," Nature Communications, Nature, vol. 15(1), pages 1-11, December.
    2. Eunbyul Cho & Youngsik Woo & Yeongjun Suh & Bo Kyoung Suh & Soo Jeong Kim & Truong Thi My Nhung & Jin Yeong Yoo & Tran Diem Nghi & Su Been Lee & Dong Jin Mun & Sang Ki Park, 2023. "Ratiometric measurement of MAM Ca2+ dynamics using a modified CalfluxVTN," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    3. Frederic Li Mow Chee & Bruno Beernaert & Billie G. C. Griffith & Alexander E. P. Loftus & Yatendra Kumar & Jimi C. Wills & Martin Lee & Jessica Valli & Ann P. Wheeler & J. Douglas Armstrong & Maddy Pa, 2023. "Mena regulates nesprin-2 to control actin–nuclear lamina associations, trans-nuclear membrane signalling and gene expression," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    4. Peng Liao & Long Chen & Hao Zhou & Jiong Mei & Ziming Chen & Bingqi Wang & Jerry Q. Feng & Guangyi Li & Sihan Tong & Jian Zhou & Siyuan Zhu & Yu Qian & Yao Zong & Weiguo Zou & Hao Li & Wenkan Zhang & , 2024. "Osteocyte mitochondria regulate angiogenesis of transcortical vessels," Nature Communications, Nature, vol. 15(1), pages 1-18, December.
    5. Shelby K. Williams & Jon Jerlström Hultqvist & Yana Eglit & Dayana E. Salas-Leiva & Bruce Curtis & Russell J. S. Orr & Courtney W. Stairs & Tuğba N. Atalay & Naomi MacMillan & Alastair G. B. Simpson &, 2024. "Extreme mitochondrial reduction in a novel group of free-living metamonads," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Julian C. Bahr & Xiao-Yan Li & Tamar Y. Feinberg & Long Jiang & Stephen J. Weiss, 2022. "Divergent regulation of basement membrane trafficking by human macrophages and cancer cells," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    7. Helene Jahn & Ladislav Bartoš & Grace I. Dearden & Jeremy S. Dittman & Joost C. M. Holthuis & Robert Vácha & Anant K. Menon, 2023. "Phospholipids are imported into mitochondria by VDAC, a dimeric beta barrel scramblase," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    8. Sarah E. Hanson & Tyrone Dowdy & Mioara Larion & Matthew Thomas Doyle & Harris D. Bernstein, 2024. "The patatin-like protein PlpD forms structurally dynamic homodimers in the Pseudomonas aeruginosa outer membrane," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    9. Xu Wang & Sarah B. Nyenhuis & Harris D. Bernstein, 2024. "The translocation assembly module (TAM) catalyzes the assembly of bacterial outer membrane proteins in vitro," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    10. Stephen M. Coscia & Andrew S. Moore & Cameron P. Thompson & Christian F. Tirrito & E. Michael Ostap & Erika L. F. Holzbaur, 2024. "An interphase actin wave promotes mitochondrial content mixing and organelle homeostasis," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    11. Chang Qiao & Yunmin Zeng & Quan Meng & Xingye Chen & Haoyu Chen & Tao Jiang & Rongfei Wei & Jiabao Guo & Wenfeng Fu & Huaide Lu & Di Li & Yuwang Wang & Hui Qiao & Jiamin Wu & Dong Li & Qionghai Dai, 2024. "Zero-shot learning enables instant denoising and super-resolution in optical fluorescence microscopy," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    12. Song Gao & Shuaibin Wang & Zhiying Zhao & Chao Zhang & Zhicao Liu & Ping Ye & Zhifang Xu & Baozhu Yi & Kai Jiao & Gurudatta A. Naik & Shi Wei & Soroush Rais-Bahrami & Sejong Bae & Wei-Hsiung Yang & Gu, 2022. "TUBB4A interacts with MYH9 to protect the nucleus during cell migration and promotes prostate cancer via GSK3β/β-catenin signalling," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    13. Ana Teresa López-Jiménez & Serge Mostowy, 2021. "Emerging technologies and infection models in cellular microbiology," Nature Communications, Nature, vol. 12(1), pages 1-13, December.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-30431-3. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.