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A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants

Author

Listed:
  • Xing-Qi Huang

    (Purdue University
    Purdue University)

  • Renqiuguo Li

    (Purdue University)

  • Jianxin Fu

    (Purdue University
    Zhejiang Agriculture & Forestry University)

  • Natalia Dudareva

    (Purdue University
    Purdue University
    Purdue University)

Abstract

Benzaldehyde, the simplest aromatic aldehyde, is one of the most wide-spread volatiles that serves as a pollinator attractant, flavor, and antifungal compound. However, the enzyme responsible for its formation in plants remains unknown. Using a combination of in vivo stable isotope labeling, classical biochemical, proteomics and genetic approaches, we show that in petunia benzaldehyde is synthesized via the β-oxidative pathway in peroxisomes by a heterodimeric enzyme consisting of α and β subunits, which belong to the NAD(P)-binding Rossmann-fold superfamily. Both subunits are alone catalytically inactive but, when mixed in equal amounts, form an active enzyme, which exhibits strict substrate specificity towards benzoyl-CoA and uses NADPH as a cofactor. Alpha subunits can form functional heterodimers with phylogenetically distant β subunits, but not all β subunits partner with α subunits, at least in Arabidopsis. Analysis of spatial, developmental and rhythmic expression of genes encoding α and β subunits revealed that expression of the gene for the α subunit likely plays a key role in regulating benzaldehyde biosynthesis.

Suggested Citation

  • Xing-Qi Huang & Renqiuguo Li & Jianxin Fu & Natalia Dudareva, 2022. "A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants," Nature Communications, Nature, vol. 13(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28978-2
    DOI: 10.1038/s41467-022-28978-2
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    References listed on IDEAS

    as
    1. Yanran Li & Christina D. Smolke, 2016. "Engineering biosynthesis of the anticancer alkaloid noscapine in yeast," Nature Communications, Nature, vol. 7(1), pages 1-14, November.
    2. Yichun Qian & Joseph H. Lynch & Longyun Guo & David Rhodes & John A. Morgan & Natalia Dudareva, 2019. "Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
    3. Heejin Yoo & Joshua R. Widhalm & Yichun Qian & Hiroshi Maeda & Bruce R. Cooper & Amber S. Jannasch & Itay Gonda & Efraim Lewinsohn & David Rhodes & Natalia Dudareva, 2013. "An alternative pathway contributes to phenylalanine biosynthesis in plants via a cytosolic tyrosine:phenylpyruvate aminotransferase," Nature Communications, Nature, vol. 4(1), pages 1-11, December.
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