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Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants

Author

Listed:
  • Yichun Qian

    (Purdue University)

  • Joseph H. Lynch

    (Purdue University)

  • Longyun Guo

    (Purdue University)

  • David Rhodes

    (Purdue University)

  • John A. Morgan

    (Purdue University
    Purdue University)

  • Natalia Dudareva

    (Purdue University
    Purdue University
    Purdue University)

Abstract

In addition to being a vital component of proteins, phenylalanine is also a precursor of numerous aromatic primary and secondary metabolites with broad physiological functions. In plants phenylalanine is synthesized predominantly via the arogenate pathway in plastids. Here, we describe the structure, molecular players and subcellular localization of a microbial-like phenylpyruvate pathway for phenylalanine biosynthesis in plants. Using a reverse genetic approach and metabolic flux analysis, we provide evidence that the cytosolic chorismate mutase is responsible for directing carbon flux towards cytosolic phenylalanine production via the phenylpyruvate pathway. We also show that an alternative transcription start site of a known plastidial enzyme produces a functional cytosolic prephenate dehydratase that catalyzes the conversion of prephenate to phenylpyruvate, the intermediate step between chorismate mutase and phenylpyruvate aminotransferase. Thus, our results complete elucidation of phenylalanine biosynthesis via phenylpyruvate in plants, showing that this pathway splits from the known plastidial arogenate pathway at chorismate, instead of prephenate as previously thought, and the complete pathway is localized in the cytosol.

Suggested Citation

  • Yichun Qian & Joseph H. Lynch & Longyun Guo & David Rhodes & John A. Morgan & Natalia Dudareva, 2019. "Completion of the cytosolic post-chorismate phenylalanine biosynthetic pathway in plants," Nature Communications, Nature, vol. 10(1), pages 1-15, December.
  • Handle: RePEc:nat:natcom:v:10:y:2019:i:1:d:10.1038_s41467-018-07969-2
    DOI: 10.1038/s41467-018-07969-2
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    Cited by:

    1. Jorge El-Azaz & Bethany Moore & Yuri Takeda-Kimura & Ryo Yokoyama & Micha Wijesingha Ahchige & Xuan Chen & Matthew Schneider & Hiroshi A. Maeda, 2023. "Coordinated regulation of the entry and exit steps of aromatic amino acid biosynthesis supports the dual lignin pathway in grasses," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    2. Xing-Qi Huang & Renqiuguo Li & Jianxin Fu & Natalia Dudareva, 2022. "A peroxisomal heterodimeric enzyme is involved in benzaldehyde synthesis in plants," Nature Communications, Nature, vol. 13(1), pages 1-15, December.

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