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DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development

Author

Listed:
  • Li He

    (Chinese Academy of Sciences)

  • Huan Huang

    (Chinese Academy of Sciences)

  • Mariem Bradai

    (Chinese Academy of Sciences)

  • Cheng Zhao

    (Chinese Academy of Sciences
    Karolinska Institutet)

  • Yin You

    (Chinese Academy of Sciences
    University of the Chinese Academy of Sciences)

  • Jun Ma

    (Chinese Academy of Sciences)

  • Lun Zhao

    (Chinese Academy of Sciences)

  • Rosa Lozano-Durán

    (Chinese Academy of Sciences
    Eberhard Karls University)

  • Jian-Kang Zhu

    (Chinese Academy of Sciences
    Southern University of Science and Technology)

Abstract

A contribution of DNA methylation to defense against invading nucleic acids and maintenance of genome integrity is uncontested; however, our understanding of the extent of involvement of this epigenetic mark in genome-wide gene regulation and plant developmental control is incomplete. Here, we knock out all five known DNA methyltransferases in Arabidopsis, generating DNA methylation-free plants. This quintuple mutant exhibits a suite of developmental defects, unequivocally demonstrating that DNA methylation is essential for multiple aspects of plant development. We show that CG methylation and non-CG methylation are required for a plethora of biological processes, including pavement cell shape, endoreduplication, cell death, flowering, trichome morphology, vasculature and meristem development, and root cell fate determination. Moreover, we find that DNA methylation has a strong dose-dependent effect on gene expression and repression of transposable elements. Taken together, our results demonstrate that DNA methylation is dispensable for Arabidopsis survival but essential for the proper regulation of multiple biological processes.

Suggested Citation

  • Li He & Huan Huang & Mariem Bradai & Cheng Zhao & Yin You & Jun Ma & Lun Zhao & Rosa Lozano-Durán & Jian-Kang Zhu, 2022. "DNA methylation-free Arabidopsis reveals crucial roles of DNA methylation in regulating gene expression and development," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    • Handle: RePEc:nat:natcom:v:13:y:2022:i:1:d:10.1038_s41467-022-28940-2
    DOI: 10.1038/s41467-022-28940-2
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    References listed on IDEAS

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    4. Kangxi Du & Jiabing Wu & Jiachen Wang & Wenhao Xie & Liufan Yin & Xiang Li & Chao Li & Aiwu Dong, 2024. "The chromatin remodeling factor OsINO80 promotes H3K27me3 and H3K9me2 deposition and maintains TE silencing in rice," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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