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Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics

Author

Listed:
  • Jonathan Schubert

    (Julius-Maximilians-University Würzburg)

  • Andrea Schulze

    (Julius-Maximilians-University Würzburg
    Proteros Biostructures)

  • Chrisostomos Prodromou

    (University of Sussex, Falmer)

  • Hannes Neuweiler

    (Julius-Maximilians-University Würzburg)

Abstract

Many proteins are molecular machines, whose function is dependent on multiple conformational changes that are initiated and tightly controlled through biochemical stimuli. Their mechanistic understanding calls for spectroscopy that can probe simultaneously such structural coordinates. Here we present two-colour fluorescence microscopy in combination with photoinduced electron transfer (PET) probes as a method that simultaneously detects two structural coordinates in single protein molecules, one colour per coordinate. This contrasts with the commonly applied resonance energy transfer (FRET) technique that requires two colours per coordinate. We demonstrate the technique by directly and simultaneously observing three critical structural changes within the Hsp90 molecular chaperone machinery. Our results reveal synchronicity of conformational motions at remote sites during ATPase-driven closure of the Hsp90 molecular clamp, providing evidence for a cooperativity mechanism in the chaperone’s catalytic cycle. Single-molecule PET fluorescence microscopy opens up avenues in the multi-dimensional exploration of protein dynamics and allosteric mechanisms.

Suggested Citation

  • Jonathan Schubert & Andrea Schulze & Chrisostomos Prodromou & Hannes Neuweiler, 2021. "Two-colour single-molecule photoinduced electron transfer fluorescence imaging microscopy of chaperone dynamics," Nature Communications, Nature, vol. 12(1), pages 1-12, December.
  • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27286-5
    DOI: 10.1038/s41467-021-27286-5
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    References listed on IDEAS

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    1. Faustine Henot & Elisa Rioual & Adrien Favier & Pavel Macek & Elodie Crublet & Pierre Josso & Bernhard Brutscher & Matthias Frech & Pierre Gans & Claire Loison & Jerome Boisbouvier, 2022. "Visualizing the transiently populated closed-state of human HSP90 ATP binding domain," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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