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The biogenesis and function of nucleosome arrays

Author

Listed:
  • Ashish Kumar Singh

    (Ludwig-Maximilians-Universität München)

  • Tamás Schauer

    (Ludwig-Maximilians-Universität München)

  • Lena Pfaller

    (Ludwig-Maximilians-Universität München
    Novartis Institutes for BioMedical Research)

  • Tobias Straub

    (Ludwig-Maximilians-Universität München)

  • Felix Mueller-Planitz

    (Ludwig-Maximilians-Universität München
    Technische Universität Dresden)

Abstract

Numerous chromatin remodeling enzymes position nucleosomes in eukaryotic cells. Aside from these factors, transcription, DNA sequence, and statistical positioning of nucleosomes also shape the nucleosome landscape. The precise contributions of these processes remain unclear due to their functional redundancy in vivo. By incisive genome engineering, we radically decreased their redundancy in Saccharomyces cerevisiae. The transcriptional machinery strongly disrupts evenly spaced nucleosomes. Proper nucleosome density and DNA sequence are critical for their biogenesis. The INO80 remodeling complex helps space nucleosomes in vivo and positions the first nucleosome over genes in an H2A.Z-independent fashion. INO80 requires its Arp8 subunit but unexpectedly not the Nhp10 module for spacing. Cells with irregularly spaced nucleosomes suffer from genotoxic stress including DNA damage, recombination and transpositions. We derive a model of the biogenesis of the nucleosome landscape and suggest that it evolved not only to regulate but also to protect the genome.

Suggested Citation

  • Ashish Kumar Singh & Tamás Schauer & Lena Pfaller & Tobias Straub & Felix Mueller-Planitz, 2021. "The biogenesis and function of nucleosome arrays," Nature Communications, Nature, vol. 12(1), pages 1-15, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-27285-6
    DOI: 10.1038/s41467-021-27285-6
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    References listed on IDEAS

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