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Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C

Author

Listed:
  • Shinya Watanabe

    (Program in Molecular Medicine, University of Massachusetts Medical School)

  • Dongyan Tan

    (Harvard Medical School
    Howard Hughes Medical Institute, Harvard Medical School)

  • Mahadevan Lakshminarasimhan

    (Stowers Institute for Medical Research)

  • Michael P. Washburn

    (Stowers Institute for Medical Research
    University of Kansas Medical Center)

  • Eun-Jin Erica Hong

    (Harvard Medical School
    Present address: ImmunoGen, Inc., 830 Winter Street, Waltham, Massachusetts 02451, USA)

  • Thomas Walz

    (Harvard Medical School
    Howard Hughes Medical Institute, Harvard Medical School)

  • Craig L. Peterson

    (Program in Molecular Medicine, University of Massachusetts Medical School)

Abstract

INO80-C and SWR-C are conserved members of a subfamily of ATP-dependent chromatin remodelling enzymes that function in transcription and genome-maintenance pathways. A crucial role for these enzymes is to control chromosomal distribution of the H2A.Z histone variant. Here we use electron microscopy (EM) and two-dimensional class averaging to demonstrate that these remodelling enzymes have similar overall architectures. Each enzyme is characterized by a dynamic ‘tail’ domain and a compact ‘head’ that contains Rvb1/Rvb2 subunits organized as hexameric rings. EM class averages and mass spectrometry support the existence of single heterohexameric rings in both SWR-C and INO80-C. EM studies define the position of the Arp8/Arp4/Act1 module within INO80-C, and we find that this module enhances nucleosome-binding affinity but is largely dispensable for remodelling activities. In contrast, the Ies6/Arp5 module is essential for INO80-C remodelling, and furthermore this module controls conformational changes that may couple nucleosome binding to remodelling.

Suggested Citation

  • Shinya Watanabe & Dongyan Tan & Mahadevan Lakshminarasimhan & Michael P. Washburn & Eun-Jin Erica Hong & Thomas Walz & Craig L. Peterson, 2015. "Structural analyses of the chromatin remodelling enzymes INO80-C and SWR-C," Nature Communications, Nature, vol. 6(1), pages 1-10, November.
    • Handle: RePEc:nat:natcom:v:6:y:2015:i:1:d:10.1038_ncomms8108
    DOI: 10.1038/ncomms8108
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    Cited by:

    1. Ashish Kumar Singh & Tamás Schauer & Lena Pfaller & Tobias Straub & Felix Mueller-Planitz, 2021. "The biogenesis and function of nucleosome arrays," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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