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INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers

Author

Listed:
  • Sandipan Brahma

    (The University of Texas MD Anderson Cancer Center
    Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center
    Graduate Program in Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine)

  • Maheshi I. Udugama

    (Graduate Program in Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine
    Present address: Department of Biochemistry and Molecular Biology, Monash University, Clayton, Victoria 3800, Australia)

  • Jongseong Kim

    (The Pennsylvania State University
    Present address: Yonsei-IBS Institute, Yonsei University, Seoul 03722, Republic of Korea)

  • Arjan Hada

    (The University of Texas MD Anderson Cancer Center
    Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center
    Graduate Program in Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine)

  • Saurabh K. Bhardwaj

    (The University of Texas MD Anderson Cancer Center
    Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center
    Graduate Program in Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine
    Present address: Division of Hematology, Children’s Hospital of Philadelphia, Philadelphia, Pennsylvania 19104, USA and Perelman School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA)

  • Solomon G. Hailu

    (The University of Texas MD Anderson Cancer Center
    Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center
    Graduate Program in Molecular Biology, Microbiology and Biochemistry, Southern Illinois University School of Medicine)

  • Tae-Hee Lee

    (The Pennsylvania State University)

  • Blaine Bartholomew

    (The University of Texas MD Anderson Cancer Center
    Center for Cancer Epigenetics, The University of Texas MD Anderson Cancer Center)

Abstract

ATP-dependent chromatin remodellers modulate nucleosome dynamics by mobilizing or disassembling nucleosomes, as well as altering nucleosome composition. These chromatin remodellers generally function by translocating along nucleosomal DNA at the H3–H4 interface of nucleosomes. Here we show that, unlike other remodellers, INO80 translocates along DNA at the H2A–H2B interface of nucleosomes and persistently displaces DNA from the surface of H2A–H2B. DNA translocation and DNA torsional strain created near the entry site of nucleosomes by INO80 promotes both the mobilization of nucleosomes and the selective exchange of H2A.Z–H2B dimers out of nucleosomes and replacement by H2A–H2B dimers without any additional histone chaperones. We find that INO80 translocates and mobilizes H2A.Z-containing nucleosomes more efficiently than those containing H2A, partially accounting for the preference of INO80 to replace H2A.Z with H2A. Our data suggest that INO80 has a mechanism for dimer exchange that is distinct from other chromatin remodellers including its paralogue SWR1.

Suggested Citation

  • Sandipan Brahma & Maheshi I. Udugama & Jongseong Kim & Arjan Hada & Saurabh K. Bhardwaj & Solomon G. Hailu & Tae-Hee Lee & Blaine Bartholomew, 2017. "INO80 exchanges H2A.Z for H2A by translocating on DNA proximal to histone dimers," Nature Communications, Nature, vol. 8(1), pages 1-12, August.
    • Handle: RePEc:nat:natcom:v:8:y:2017:i:1:d:10.1038_ncomms15616
    DOI: 10.1038/ncomms15616
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    Cited by:

    1. Ashish Kumar Singh & Tamás Schauer & Lena Pfaller & Tobias Straub & Felix Mueller-Planitz, 2021. "The biogenesis and function of nucleosome arrays," Nature Communications, Nature, vol. 12(1), pages 1-15, December.

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