IDEAS home Printed from https://ideas.repec.org/a/nat/natcom/v12y2021i1d10.1038_s41467-021-26381-x.html
   My bibliography  Save this article

A three-tiered colloidosomal microreactor for continuous flow catalysis

Author

Listed:
  • Hua Wu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Xuanlin Du

    (Chinese Academy of Sciences)

  • Xiaohui Meng

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Dong Qiu

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

  • Yan Qiao

    (Chinese Academy of Sciences
    University of Chinese Academy of Sciences)

Abstract

Integrative colloidosomes with hierarchical structure and advanced function may serve as biomimetic microreactors to carry out catalytic reactions by compartmentalizing biological species within semipermeable membranes. Despite of recent progress in colloidosome design, integration of biological and inorganic components into tiered structures to tackle the remaining challenges of biocatalysis is highly demanded. Here, we report a rational design of three-tiered colloidosomes via the Pickering emulsion process. The microreactor consists of crosslinked amphiphilic silica-polymer hybrid nanoparticles as the semipermeable shell, an enzyme-incorporated catalytic sub-layer, and a partially-silicified adsorptive lumen. By leveraging confinement and enrichment effect, we demonstrate the acceleration of lipase-catalyzed ester hydrolysis within the microcompartment of organic-inorganic hybrid colloidosomes. The catalytic colloidosomes are further assembled into a closely packed column for enzymatic reactions in a continuous flow format with enhanced reaction rates. The three-tiered colloidosomes provide a reliable platform to integrate functional building blocks into a biomimetic compartmentalized microreactor with spatially controlled organization and high-performance functions.

Suggested Citation

  • Hua Wu & Xuanlin Du & Xiaohui Meng & Dong Qiu & Yan Qiao, 2021. "A three-tiered colloidosomal microreactor for continuous flow catalysis," Nature Communications, Nature, vol. 12(1), pages 1-8, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26381-x
    DOI: 10.1038/s41467-021-26381-x
    as

    Download full text from publisher

    File URL: https://www.nature.com/articles/s41467-021-26381-x
    File Function: Abstract
    Download Restriction: no
    File URL: https://libkey.io/10.1038/s41467-021-26381-x?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    References listed on IDEAS

    as
    1. Roberta Lentini & Silvia Perez Santero & Fabio Chizzolini & Dario Cecchi & Jason Fontana & Marta Marchioretto & Cristina Del Bianco & Jessica L. Terrell & Amy C. Spencer & Laura Martini & Michele Forl, 2014. "Integrating artificial with natural cells to translate chemical messages that direct E. coli behaviour," Nature Communications, Nature, vol. 5(1), pages 1-6, September.
    2. Sheref S. Mansy & Jason P. Schrum & Mathangi Krishnamurthy & Sylvia Tobé & Douglas A. Treco & Jack W. Szostak, 2008. "Template-directed synthesis of a genetic polymer in a model protocell," Nature, Nature, vol. 454(7200), pages 122-125, July.
    3. Pierangelo Gobbo & Liangfei Tian & B. V. V. S Pavan Kumar & Samuel Turvey & Mattia Cattelan & Avinash J. Patil & Mauro Carraro & Marcella Bonchio & Stephen Mann, 2020. "Catalytic processing in ruthenium-based polyoxometalate coacervate protocells," Nature Communications, Nature, vol. 11(1), pages 1-9, December.
    4. Kensuke Kurihara & Yusaku Okura & Muneyuki Matsuo & Taro Toyota & Kentaro Suzuki & Tadashi Sugawara, 2015. "A recursive vesicle-based model protocell with a primitive model cell cycle," Nature Communications, Nature, vol. 6(1), pages 1-7, December.
    5. Xin Huang & Mei Li & David C. Green & David S. Williams & Avinash J. Patil & Stephen Mann, 2013. "Interfacial assembly of protein–polymer nano-conjugates into stimulus-responsive biomimetic protocells," Nature Communications, Nature, vol. 4(1), pages 1-9, October.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Songyang Liu & Yanwen Zhang & Xiaoxiao He & Mei Li & Jin Huang & Xiaohai Yang & Kemin Wang & Stephen Mann & Jianbo Liu, 2022. "Signal processing and generation of bioactive nitric oxide in a model prototissue," Nature Communications, Nature, vol. 13(1), pages 1-12, December.
    2. Omer Adir & Mia R. Albalak & Ravit Abel & Lucien E. Weiss & Gal Chen & Amit Gruber & Oskar Staufer & Yaniv Kurman & Ido Kaminer & Jeny Shklover & Janna Shainsky-Roitman & Ilia Platzman & Lior Gepstein, 2022. "Synthetic cells with self-activating optogenetic proteins communicate with natural cells," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    3. Adrian Zambrano & Giorgio Fracasso & Mengfei Gao & Martina Ugrinic & Dishi Wang & Dietmar Appelhans & Andrew deMello & T-Y. Dora Tang, 2022. "Programmable synthetic cell networks regulated by tuneable reaction rates," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    4. Shang Dai & Zhenming Xie & Binqiang Wang & Rui Ye & Xinwen Ou & Chen Wang & Ning Yu & Cheng Huang & Jie Zhao & Chunhui Cai & Furong Zhang & Damiano Buratto & Taimoor Khan & Yan Qiao & Yuejin Hua & Ruh, 2023. "An inorganic mineral-based protocell with prebiotic radiation fitness," Nature Communications, Nature, vol. 14(1), pages 1-14, December.
    5. Shoupeng Cao & Tsvetomir Ivanov & Julian Heuer & Calum T. J. Ferguson & Katharina Landfester & Lucas Caire da Silva, 2024. "Dipeptide coacervates as artificial membraneless organelles for bioorthogonal catalysis," Nature Communications, Nature, vol. 15(1), pages 1-14, December.
    6. Tony Z. Jia & Yutetsu Kuruma, 2019. "Recent Advances in Origins of Life Research by Biophysicists in Japan," Challenges, MDPI, vol. 10(1), pages 1-21, April.

    More about this item

    Statistics

    Access and download statistics

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26381-x. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Sonal Shukla or Springer Nature Abstracting and Indexing (email available below). General contact details of provider: http://www.nature.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.