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High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes

Author

Listed:
  • Hyun Ji An

    (University of Seoul)

  • Yun Kim

    (Hanbat National University)

  • Soojeong Chang

    (University of Seoul)

  • Hakchun Kim

    (University of Seoul)

  • Jihwan Song

    (Hanbat National University)

  • Hyunsung Park

    (University of Seoul)

  • Inhee Choi

    (University of Seoul)

Abstract

Histones are closely related to the state of chromatin, and epigenetic modification of their tail results in regulation in cells. Therefore, developing various analytical tools to map the changes in position and distribution of histone modifications is helpful in studying underlying mechanisms. Herein, we propose a high-spatial and colourimetric imaging method using plasmonic nanoparticles as probes to visualize heterochromatin histone markers in a single nucleus. We visualized the reorganization between repressive histone markers, H3K9me3 and H3K27me3, caused by oncogene-induced senescence based on the scattering colours and spectral shift of plasmonic nanoprobes to longer wavelengths using their distance-dependent coupling effect. The measured scattering profiles were correlated with the computation results simulating the scattering spectra according to the arrangements and distances among the plasmonic nanoprobes. The plasmonic nanoprobe-based high-spatial hyperspectral imaging provides an advanced way to study the dynamics of histone modifications for predicting the progression of diseases or senescence.

Suggested Citation

  • Hyun Ji An & Yun Kim & Soojeong Chang & Hakchun Kim & Jihwan Song & Hyunsung Park & Inhee Choi, 2021. "High-spatial and colourimetric imaging of histone modifications in single senescent cells using plasmonic nanoprobes," Nature Communications, Nature, vol. 12(1), pages 1-11, December.
    • Handle: RePEc:nat:natcom:v:12:y:2021:i:1:d:10.1038_s41467-021-26224-9
    DOI: 10.1038/s41467-021-26224-9
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    References listed on IDEAS

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    1. Jesse R. Dixon & Siddarth Selvaraj & Feng Yue & Audrey Kim & Yan Li & Yin Shen & Ming Hu & Jun S. Liu & Bing Ren, 2012. "Topological domains in mammalian genomes identified by analysis of chromatin interactions," Nature, Nature, vol. 485(7398), pages 376-380, May.
    2. Ulrike C. Lange & Stéphanie Siebert & Mark Wossidlo & Thomas Weiss & Céline Ziegler-Birling & Jörn Walter & Maria-Elena Torres-Padilla & Sylvain Daujat & Robert Schneider, 2013. "Dissecting the role of H3K64me3 in mouse pericentromeric heterochromatin," Nature Communications, Nature, vol. 4(1), pages 1-10, October.
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