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Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle

Author

Listed:
  • Philipp Keller

    (Institute of Microbiology, Department of Biology, ETH Zurich)

  • Elad Noor

    (Institute of Molecular Systems Biology, Department of Biology, ETH Zurich)

  • Fabian Meyer

    (Institute of Microbiology, Department of Biology, ETH Zurich)

  • Michael A. Reiter

    (Institute of Microbiology, Department of Biology, ETH Zurich)

  • Stanislav Anastassov

    (Institute of Microbiology, Department of Biology, ETH Zurich)

  • Patrick Kiefer

    (Institute of Microbiology, Department of Biology, ETH Zurich)

  • Julia A. Vorholt

    (Institute of Microbiology, Department of Biology, ETH Zurich)

Abstract

Methanol is a biotechnologically promising substitute for food and feed substrates since it can be produced renewably from electricity, water and CO2. Although progress has been made towards establishing Escherichia coli as a platform organism for methanol conversion via the energy efficient ribulose monophosphate (RuMP) cycle, engineering strains that rely solely on methanol as a carbon source remains challenging. Here, we apply flux balance analysis to comprehensively identify methanol-dependent strains with high potential for adaptive laboratory evolution. We further investigate two out of 1200 candidate strains, one with a deletion of fructose-1,6-bisphosphatase (fbp) and another with triosephosphate isomerase (tpiA) deleted. In contrast to previous reported methanol-dependent strains, both feature a complete RuMP cycle and incorporate methanol to a high degree, with up to 31 and 99% fractional incorporation into RuMP cycle metabolites. These strains represent ideal starting points for evolution towards a fully methylotrophic lifestyle.

Suggested Citation

  • Philipp Keller & Elad Noor & Fabian Meyer & Michael A. Reiter & Stanislav Anastassov & Patrick Kiefer & Julia A. Vorholt, 2020. "Methanol-dependent Escherichia coli strains with a complete ribulose monophosphate cycle," Nature Communications, Nature, vol. 11(1), pages 1-10, December.
    • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-19235-5
    DOI: 10.1038/s41467-020-19235-5
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    Cited by:

    1. Enrico Orsi & Pablo Ivan Nikel & Lars Keld Nielsen & Stefano Donati, 2023. "Synergistic investigation of natural and synthetic C1-trophic microorganisms to foster a circular carbon economy," Nature Communications, Nature, vol. 14(1), pages 1-9, December.
    2. Philipp Keller & Michael A. Reiter & Patrick Kiefer & Thomas Gassler & Lucas Hemmerle & Philipp Christen & Elad Noor & Julia A. Vorholt, 2022. "Generation of an Escherichia coli strain growing on methanol via the ribulose monophosphate cycle," Nature Communications, Nature, vol. 13(1), pages 1-13, December.

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