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Size and surface charge characterization of nanoparticles with a salt gradient

Author

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  • Martin K. Rasmussen

    (Technical University of Denmark)

  • Jonas N. Pedersen

    (Technical University of Denmark)

  • Rodolphe Marie

    (Technical University of Denmark)

Abstract

Exosomes are nanometer-sized lipid vesicles present in liquid biopsies and used as biomarkers for several diseases including cancer, Alzheimer’s, and central nervous system diseases. Purification and subsequent size and surface characterization are essential to exosome-based diagnostics. Sample purification is, however, time consuming and potentially damaging, and no current method gives the size and zeta potential from a single measurement. Here, we concentrate exosomes from a dilute solution and measure their size and zeta potential in a one-step measurement with a salt gradient in a capillary channel. The salt gradient causes oppositely directed particle and fluid transport that trap particles. Within minutes, the particle concentration increases more than two orders of magnitude. A fit to the spatial distribution of a single or an ensemble of exosomes returns both their size and surface charge. Our method is applicable for other types of nanoparticles. The capillary is fabricated in a low-cost polymer device.

Suggested Citation

  • Martin K. Rasmussen & Jonas N. Pedersen & Rodolphe Marie, 2020. "Size and surface charge characterization of nanoparticles with a salt gradient," Nature Communications, Nature, vol. 11(1), pages 1-8, December.
  • Handle: RePEc:nat:natcom:v:11:y:2020:i:1:d:10.1038_s41467-020-15889-3
    DOI: 10.1038/s41467-020-15889-3
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    Cited by:

    1. Viet Sang Doan & Ibraheem Alshareedah & Anurag Singh & Priya R. Banerjee & Sangwoo Shin, 2024. "Diffusiophoresis promotes phase separation and transport of biomolecular condensates," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    2. Suin Shim & Bernardo Gouveia & Beatrice Ramm & Venecia A. Valdez & Sabine Petry & Howard A. Stone, 2024. "Motorless transport of microtubules along tubulin, RanGTP, and salt gradients," Nature Communications, Nature, vol. 15(1), pages 1-12, December.
    3. Dolachai Boniface & Sergi G. Leyva & Ignacio Pagonabarraga & Pietro Tierno, 2024. "Clustering induces switching between phoretic and osmotic propulsion in active colloidal rafts," Nature Communications, Nature, vol. 15(1), pages 1-11, December.

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