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Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods

Author

Listed:
  • Kang Zhao

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
    China National Publications Import & Export (Group) Corporation, Beijing 100020, China)

  • Qinjian Di

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)

  • Xi Cao

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)

  • Meng Wang

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China)

  • Li Deng

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
    Amoy—BUCT Industrial Bio-technovation Institute, Amoy 361022, China)

  • Fang Wang

    (Beijing Bioprocess Key Laboratory, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing 100029, China
    State Key Laboratory of Chemical Resource Engineering, Beijing University of Chemical Technology, Beijing 100029, China)

Abstract

Lipase from Candida sp. 99–125 is widely employed to catalyzed transesterification and can be used for biodiesel production. In this study, the lipase was immobilized by combined adsorption and entrapment to catalyze biodiesel production from waste cooking oil (WCO) via transesterification, and investigating co-immobilizing agents as additives according to the enzyme activity. The addition of the mixed co-immobilizing agents has positive effects on the activities of the immobilized lipase. Three different immobilizing methods were compared by the conversion ratio of biodiesel and structured by Atom Force Microscopy (AFM) and Scanning Electron Microscopy (SEM), respectively. It was found that entrapment followed by adsorption was the best method. The effect of the co-immobilizing agent amount, lipase dosage, water content, and reuse ability of the immobilized lipase was investigated. By comparison with previous research, this immobilized lipase showed good reuse ability: the conversion ratio excesses 70% after 10 subsequent reactions, in particular, was better than Novozym435 and TLIM on waste cooking oil for one unit of lipase.

Suggested Citation

  • Kang Zhao & Qinjian Di & Xi Cao & Meng Wang & Li Deng & Fang Wang, 2016. "Production of Biodiesel Using Immobilized Lipase and the Characterization of Different Co-Immobilizing Agents and Immobilization Methods," Sustainability, MDPI, vol. 8(9), pages 1-11, August.
    • Handle: RePEc:gam:jsusta:v:8:y:2016:i:9:p:764-:d:76587
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    References listed on IDEAS

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    1. Gog, Adriana & Roman, Marius & Toşa, Monica & Paizs, Csaba & Irimie, Florin Dan, 2012. "Biodiesel production using enzymatic transesterification – Current state and perspectives," Renewable Energy, Elsevier, vol. 39(1), pages 10-16.
    2. Demirbas, Ayhan, 2009. "Political, economic and environmental impacts of biofuels: A review," Applied Energy, Elsevier, vol. 86(Supplemen), pages 108-117, November.
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    Cited by:

    1. Dash, Archana & Banerjee, Rintu, 2021. "Exploring indigenously produced celite-immobilized Rhizopus oryzae NRRL 3562-lipase for biodiesel production," Energy, Elsevier, vol. 222(C).
    2. Dacinia Crina Petrescu & Ruxandra Mălina Petrescu-Mag & Dorin Iosif Manciula & Ioan Alin Nistor & Veronica Ioana Ilieș, 2018. "Wastewater Reflections in Consumer Mind: Evidence from Sewage Services Consumer Behaviour," Sustainability, MDPI, vol. 11(1), pages 1-15, December.

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