IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v15y2023i21p15247-d1266781.html
   My bibliography  Save this article

Enrichment of Microbial Consortium with Hydrogenotrophic Methanogens for Biological Biogas Upgrade to Biomethane in a Bubble Reactor under Mesophilic Conditions

Author

Listed:
  • Apostolos Spyridonidis

    (Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, GR-67132 Xanthi, Greece)

  • Ioanna A. Vasiliadou

    (Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, GR-67132 Xanthi, Greece
    Department of Chemical Engineering, University of Western Macedonia, GR-50100 Kozani, Greece)

  • Panagiota Stathopoulou

    (Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, G. Seferi 2, GR-30131 Agrinio, Greece)

  • Athanasios Tsiamis

    (Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, G. Seferi 2, GR-30131 Agrinio, Greece)

  • George Tsiamis

    (Laboratory of Systems Microbiology and Applied Genomics, Department of Sustainable Agriculture, University of Patras, G. Seferi 2, GR-30131 Agrinio, Greece)

  • Katerina Stamatelatou

    (Department of Environmental Engineering, Democritus University of Thrace, Vas. Sofias 12, GR-67132 Xanthi, Greece)

Abstract

The biological upgrading of biogas to simulate natural gas properties contributes to the sustainable establishment of biogas technology. It is an alternative technology to the conventional physicochemical methods applied in biomethane plants and has been studied mainly in thermophilic conditions. Developing an enriched culture for converting the CO 2 of biogas to CH 4 in mesophilic conditions was the subject of the present study, which could facilitate the biological process and establish it in the mesophilic range of temperature. The enrichment took place via successive dilutions in a bubble bioreactor operated in fed-batch mode. The methane percentage was recorded at 95.5 ± 1.2% until the end of the experiment. The methane production rate was 0.28–0.30 L L −1 d −1 following the low hydrogen loading rate (1.2 ± 0.1 L L −1 d −1 ) applied to avoid acetate accumulation. Hydrogenotrophic methanogens, Methanobrevibacter sp., were identified at a proportion of 97.9% among the Archaea and 60% of the total population of the enriched culture. Moreover, homoacetogens ( Sporomusa sp.) and acetate oxidizers ( Proteiniphilum sp.) were also detected, indicating that a possible metabolic pathway for CH 4 production from CO 2 is via homoacetogenesis and syntrophic acetate oxidation, which kept the acetate concentration at a level of 143 ± 13 mg L −1 . It was found that adding NaHCO 3 was adequate to sustain the pH at 8.25.

Suggested Citation

  • Apostolos Spyridonidis & Ioanna A. Vasiliadou & Panagiota Stathopoulou & Athanasios Tsiamis & George Tsiamis & Katerina Stamatelatou, 2023. "Enrichment of Microbial Consortium with Hydrogenotrophic Methanogens for Biological Biogas Upgrade to Biomethane in a Bubble Reactor under Mesophilic Conditions," Sustainability, MDPI, vol. 15(21), pages 1-19, October.
    • Handle: RePEc:gam:jsusta:v:15:y:2023:i:21:p:15247-:d:1266781
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/15/21/15247/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/15/21/15247/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Taifouris, Manuel & Martín, Mariano, 2023. "Towards energy security by promoting circular economy: A holistic approach," Applied Energy, Elsevier, vol. 333(C).
    2. Rachbauer, Lydia & Voitl, Gregor & Bochmann, Günther & Fuchs, Werner, 2016. "Biological biogas upgrading capacity of a hydrogenotrophic community in a trickle-bed reactor," Applied Energy, Elsevier, vol. 180(C), pages 483-490.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Khan, Muhammad Usman & Lee, Jonathan Tian En & Bashir, Muhammad Aamir & Dissanayake, Pavani Dulanja & Ok, Yong Sik & Tong, Yen Wah & Shariati, Mohammad Ali & Wu, Sarah & Ahring, Birgitte Kiaer, 2021. "Current status of biogas upgrading for direct biomethane use: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 149(C).
    2. Martin Thema & Tobias Weidlich & Manuel Hörl & Annett Bellack & Friedemann Mörs & Florian Hackl & Matthias Kohlmayer & Jasmin Gleich & Carsten Stabenau & Thomas Trabold & Michael Neubert & Felix Ortlo, 2019. "Biological CO 2 -Methanation: An Approach to Standardization," Energies, MDPI, vol. 12(9), pages 1-32, May.
    3. Zheng, Lei & Cheng, Shikun & Han, Yanzhao & Wang, Min & Xiang, Yue & Guo, Jiali & Cai, Di & Mang, Heinz-Peter & Dong, Taili & Li, Zifu & Yan, Zhengxu & Men, Yu, 2020. "Bio-natural gas industry in China: Current status and development," Renewable and Sustainable Energy Reviews, Elsevier, vol. 128(C).
    4. Voelklein, M.A. & Rusmanis, Davis & Murphy, J.D., 2019. "Biological methanation: Strategies for in-situ and ex-situ upgrading in anaerobic digestion," Applied Energy, Elsevier, vol. 235(C), pages 1061-1071.
    5. Grimalt-Alemany, Antonio & Asimakopoulos, Konstantinos & Skiadas, Ioannis V. & Gavala, Hariklia N., 2020. "Modeling of syngas biomethanation and catabolic route control in mesophilic and thermophilic mixed microbial consortia," Applied Energy, Elsevier, vol. 262(C).
    6. Rocío González-Sánchez & Sara Alonso-Muñoz & María Sonia Medina-Salgado, 2023. "Circularity in waste management: a research proposal to achieve the 2030 Agenda," Operations Management Research, Springer, vol. 16(3), pages 1520-1540, September.
    7. Park, Jun-Gyu & Kwon, Hye-Jeong & Cheon, A-In & Jun, Hang-Bae, 2021. "Jet-nozzle based improvement of dissolved H2 concentration for efficient in-situ biogas upgrading in an up-flow anaerobic sludge blanket (UASB) reactor," Renewable Energy, Elsevier, vol. 168(C), pages 270-279.
    8. Witte, Julia & Calbry-Muzyka, Adelaide & Wieseler, Tanja & Hottinger, Peter & Biollaz, Serge M.A. & Schildhauer, Tilman J., 2019. "Demonstrating direct methanation of real biogas in a fluidised bed reactor," Applied Energy, Elsevier, vol. 240(C), pages 359-371.
    9. Wu, Benteng & Lin, Richen & Kang, Xihui & Deng, Chen & Dobson, Alan D.W. & Murphy, Jerry D., 2021. "Improved robustness of ex-situ biological methanation for electro-fuel production through the addition of graphene," Renewable and Sustainable Energy Reviews, Elsevier, vol. 152(C).
    10. Andreas Lemmer & Timo Ullrich, 2018. "Effect of Different Operating Temperatures on the Biological Hydrogen Methanation in Trickle Bed Reactors," Energies, MDPI, vol. 11(6), pages 1-11, May.
    11. Strübing, Dietmar & Moeller, Andreas B. & Mößnang, Bettina & Lebuhn, Michael & Drewes, Jörg E. & Koch, Konrad, 2018. "Anaerobic thermophilic trickle bed reactor as a promising technology for flexible and demand-oriented H2/CO2 biomethanation," Applied Energy, Elsevier, vol. 232(C), pages 543-554.
    12. Kirchbacher, F. & Miltner, M. & Wukovits, W. & Harasek, M., 2019. "Economic assessment of membrane-based power-to-gas processes for the European biogas market," Renewable and Sustainable Energy Reviews, Elsevier, vol. 112(C), pages 338-352.
    13. Yun, Yeo-Myeong & Sung, Shihwu & Kang, Seoktae & Kim, Mi-Sun & Kim, Dong-Hoon, 2017. "Enrichment of hydrogenotrophic methanogens by means of gas recycle and its application in biogas upgrading," Energy, Elsevier, vol. 135(C), pages 294-302.
    14. Savvas, Savvas & Donnelly, Joanne & Patterson, Tim & Chong, Zyh S. & Esteves, Sandra R., 2017. "Biological methanation of CO2 in a novel biofilm plug-flow reactor: A high rate and low parasitic energy process," Applied Energy, Elsevier, vol. 202(C), pages 238-247.
    15. Wantz, Eliot & Benizri, David & Dietrich, Nicolas & Hébrard, Gilles, 2022. "Rate-based modeling approach for High Pressure Water Scrubbing with unsteady gas flowrate and multicomponent absorption applied to biogas upgrading," Applied Energy, Elsevier, vol. 312(C).
    16. Eduardo Sánchez Nocete & Javier Pérez Rodríguez, 2022. "A Simple Methodology for Estimating the Potential Biomethane Production in a Region: Application in a Case Study," Sustainability, MDPI, vol. 14(23), pages 1-18, November.
    17. Tuğçe Dağlıoğlu & Tuba Ceren Öğüt & Guven Ozdemir & Nuri Azbar, 2021. "Comparative analysis of the effect of cell immobilization on the hydrogenothrophic biomethanation of CO2," Greenhouse Gases: Science and Technology, Blackwell Publishing, vol. 11(3), pages 493-505, June.
    18. Lee, Eun Seo & Park, Seon Yeong & Kim, Chang Gyun, 2023. "Feasibility test anaerobically enhancing methane yield under the injection of hydrogen and carbon dioxide," Renewable Energy, Elsevier, vol. 212(C), pages 761-768.
    19. Jensen, M.B. & Ottosen, L.D.M. & Kofoed, M.V.W., 2021. "H2 gas-liquid mass transfer: A key element in biological Power-to-Gas methanation," Renewable and Sustainable Energy Reviews, Elsevier, vol. 147(C).
    20. Burkhardt, Marko & Jordan, Isabel & Heinrich, Sabrina & Behrens, Johannes & Ziesche, André & Busch, Günter, 2019. "Long term and demand-oriented biocatalytic synthesis of highly concentrated methane in a trickle bed reactor," Applied Energy, Elsevier, vol. 240(C), pages 818-826.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:15:y:2023:i:21:p:15247-:d:1266781. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.