IDEAS home Printed from https://ideas.repec.org/a/gam/jsusta/v15y2022i1p505-d1017604.html
   My bibliography  Save this article

Enhanced Bioconversion of Methane to Biodiesel by Methylosarcina sp. LC-4

Author

Listed:
  • Nivedita Sana

    (Department of Civil Engineering, Indian Institute of Technology Madras, Chennai 600036, India)

  • Dali Naidu Arnepalli

    (Department of Civil Engineering, Indian Institute of Technology Madras, Chennai 600036, India)

  • Chandraraj Krishnan

    (Department of Biotechnology, Indian Institute of Technology Madras, Chennai 600036, India)

Abstract

The conversion of methane into liquid biofuels using methane-consuming bacteria, known as methanotrophs, contributes to sustainable development, as it mitigates the problem of climate change caused by greenhouse gases and aids in producing cleaner and renewable energy. In the present research, an efficient methanotroph, Methylosarcina sp. LC-4, was studied as a prospective organism for biodiesel production using methane. The methane uptake rate by the organism was enhanced 1.6 times and 2.35 times by supplementing LC-4 with micronutrients, such as copper and tungstate, respectively. This unique ability of the isolated organism enables the deployment of methanotrophs-based processes in various industrial applications. A Plackett–Burman statistical (PBD) design was used to quantify the role of the micronutrients and other media components present in the nitrate minimal salt media (NMS) in biomass and fatty acid methyl esters (FAME) yields. Nitrate, phosphate, and tungstate had a positive effect, whereas copper, magnesium, and salinity had a negative effect. The modified NMS media, formulated according to the results from the PBD analysis, increased the FAME yield (mg/L) by 85.7%, with the FAME content of 13 ± 1% ( w / w ) among the highest reported in methanotrophs. The obtained FAME consisted majorly (~90%) of C 14 –C 18 saturated and monounsaturated fatty acids, making it suitable for use as biodiesel.

Suggested Citation

  • Nivedita Sana & Dali Naidu Arnepalli & Chandraraj Krishnan, 2022. "Enhanced Bioconversion of Methane to Biodiesel by Methylosarcina sp. LC-4," Sustainability, MDPI, vol. 15(1), pages 1-15, December.
    • Handle: RePEc:gam:jsusta:v:15:y:2022:i:1:p:505-:d:1017604
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/2071-1050/15/1/505/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/2071-1050/15/1/505/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. AlSayed, Ahmed & Fergala, Ahmed & Khattab, Saif & ElSharkawy, Adham & Eldyasti, Ahmed, 2018. "Optimization of methane bio-hydroxylation using waste activated sludge mixed culture of type I methanotrophs as biocatalyst," Applied Energy, Elsevier, vol. 211(C), pages 755-763.
    2. Karakurt, Izzet & Aydin, Gokhan & Aydiner, Kerim, 2012. "Sources and mitigation of methane emissions by sectors: A critical review," Renewable Energy, Elsevier, vol. 39(1), pages 40-48.
    3. Alex Zappi & Dhan Lord Fortela & William E. Holmes, 2020. "An Assessment of Methanotrophs Producing Industrial-Grade Lipids for Biofuels and Other Commercial Chemicals," Energies, MDPI, vol. 13(15), pages 1-17, July.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Kim, Dongin & Han, Jeehoon, 2020. "Techno-economic and climate impact analysis of carbon utilization process for methanol production from blast furnace gas over Cu/ZnO/Al2O3 catalyst," Energy, Elsevier, vol. 198(C).
    2. Kang, Mary & Mauzerall, Denise L. & Ma, Daniel Z. & Celia, Michael A., 2019. "Reducing methane emissions from abandoned oil and gas wells: Strategies and costs," Energy Policy, Elsevier, vol. 132(C), pages 594-601.
    3. Wang, Bin & Liu, Shuyang & Wang, Pengfei, 2022. "Microwave-assisted high-efficient gas production of depressurization-induced methane hydrate exploitation," Energy, Elsevier, vol. 247(C).
    4. Georgios Varvoutis & Athanasios Lampropoulos & Evridiki Mandela & Michalis Konsolakis & George E. Marnellos, 2022. "Recent Advances on CO 2 Mitigation Technologies: On the Role of Hydrogenation Route via Green H 2," Energies, MDPI, vol. 15(13), pages 1-38, June.
    5. Ahmadi, Ehsan & Yousefzadeh, Samira & Mokammel, Adel & Miri, Mohammad & Ansari, Mohsen & Arfaeinia, Hossein & Badi, Mojtaba Yegane & Ghaffari, Hamid Reza & Rezaei, Soheila & Mahvi, Amir Hossein, 2020. "Kinetic study and performance evaluation of an integrated two-phase fixed-film baffled bioreactor for bioenergy recovery from wastewater and bio-wasted sludge," Renewable and Sustainable Energy Reviews, Elsevier, vol. 121(C).
    6. Patel, Sanjay K.S. & Kondaveeti, Sanath & Otari, Sachin V. & Pagolu, Ravi T. & Jeong, Seong Hun & Kim, Sun Chang & Cho, Byung-Kwan & Kang, Yun Chan & Lee, Jung-Kul, 2018. "Repeated batch methanol production from a simulated biogas mixture using immobilized Methylocystis bryophila," Energy, Elsevier, vol. 145(C), pages 477-485.
    7. Fernández-Amador, Octavio & Francois, Joseph F. & Oberdabernig, Doris A. & Tomberger, Patrick, 2020. "The methane footprint of nations: Stylized facts from a global panel dataset," Ecological Economics, Elsevier, vol. 170(C).
    8. Jiang, Haipeng & Bi, Mingshu & Gao, Zehua & Zhang, Zongling & Gao, Wei, 2022. "Effect of turbulence intensity on flame propagation and extinction limits of methane/coal dust explosions," Energy, Elsevier, vol. 239(PC).
    9. Anisur, M.R. & Mahfuz, M.H. & Kibria, M.A. & Saidur, R. & Metselaar, I.H.S.C. & Mahlia, T.M.I., 2013. "Curbing global warming with phase change materials for energy storage," Renewable and Sustainable Energy Reviews, Elsevier, vol. 18(C), pages 23-30.
    10. Zhang, Bo & Chen, G.Q., 2014. "Methane emissions in China 2007," Renewable and Sustainable Energy Reviews, Elsevier, vol. 30(C), pages 886-902.
    11. Aydin, Gokhan, 2014. "Modeling of energy consumption based on economic and demographic factors: The case of Turkey with projections," Renewable and Sustainable Energy Reviews, Elsevier, vol. 35(C), pages 382-389.
    12. Shakila Aziz & Shahriar Ahmed Chowdhury, 2023. "Analysis of agricultural greenhouse gas emissions using the STIRPAT model: a case study of Bangladesh," Environment, Development and Sustainability: A Multidisciplinary Approach to the Theory and Practice of Sustainable Development, Springer, vol. 25(5), pages 3945-3965, May.
    13. Figaj, Rafał, 2021. "Performance assessment of a renewable micro-scale trigeneration system based on biomass steam cycle, wind turbine, photovoltaic field," Renewable Energy, Elsevier, vol. 177(C), pages 193-208.
    14. Nicoletta Brazzola & Jan Wohland & Anthony Patt, 2021. "Offsetting unabated agricultural emissions with CO2 removal to achieve ambitious climate targets," PLOS ONE, Public Library of Science, vol. 16(3), pages 1-19, March.
    15. Bai, Yang & Lin, Hai-Fei & Li, Shu-Gang & Long, Hang & Yan, Min & Li, Yong & Qin, Lei & Zhou, Bin, 2022. "Experimental study on kinetic characteristics of gas diffusion in coal under nitrogen injection," Energy, Elsevier, vol. 254(PA).
    16. Bo Lan & Peng-Fei Gao & You-Rong Li & Jia-Jia Yu & Peng-Cheng Li, 2022. "Numerical Simulation and Theoretical Analysis of Flow Resistance Characteristics in the Honeycomb Ceramic Conduit," Energies, MDPI, vol. 15(19), pages 1-14, October.
    17. Le Qi & Hai-Dong Niu & Peng Zhou & Rui-Jie Jia & Ming Gao, 2018. "Effects of Biochar on the Net Greenhouse Gas Emissions under Continuous Flooding and Water-Saving Irrigation Conditions in Paddy Soils," Sustainability, MDPI, vol. 10(5), pages 1-17, May.
    18. Koen De Vos & Charlotte Janssens & Liesbet Jacobs & Benjamin Campforts & Esther Boere & Marta Kozicka & David Leclère & Petr Havlík & Lisa-Marie Hemerijckx & Anton Van Rompaey & Miet Maertens & Gerard, 2024. "African food system and biodiversity mainly affected by urbanization via dietary shifts," Nature Sustainability, Nature, vol. 7(7), pages 869-878, July.
    19. Yusuf, Rafiu O. & Noor, Zainura Z. & Abba, Ahmad H. & Hassan, Mohd Ariffin Abu & Din, Mohd Fadhil Mohd, 2012. "Methane emission by sectors: A comprehensive review of emission sources and mitigation methods," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(7), pages 5059-5070.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jsusta:v:15:y:2022:i:1:p:505-:d:1017604. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.