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Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine

Author

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  • Ningning Li

    (College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China)

  • Bingjing Hu

    (College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China)

  • Anming Wang

    (College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China)

  • Huimin Li

    (College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China)

  • Youcheng Yin

    (Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou 311121, China)

  • Tianyu Mao

    (College of Materials, Chemistry and Chemical Engineering, Hangzhou Normal University, Hangzhou 311121, China)

  • Tian Xie

    (Holistic Integrative Pharmacy Institutes, College of Medicine, Hangzhou Normal University, Hangzhou 311121, China)

Abstract

To develop an environmentally friendly biocatalyst for the efficient synthesis of organofluorine compounds, we prepared the enzyme@fluoridated hydroxyapatite nanoflowers (FHAp-NFs) using fluorinase expressed in Escherichia coli Rosetta (DE3) as the biomineralization framework. The obtained fluorinase@FHAp-NFs were characterized by scanning electron microscope (SEM), X-ray diffraction (XRD), and FT-IR spectrum and used in the enzymatic synthesis of 5′-fluorodeoxy adenosin with S-adenosyl-L-methionine and fluoride as substrate. At an optimum pH of 7.5, fluorinase confined in the hybrid nanoflowers presents an approximately 2-fold higher synthetic activity than free fluorinase. Additionally, after heating at 30 °C for 8 h, the FHAp-NFs retained approximately 80.0% of the initial activity. However, free enzyme could remain only 48.2% of its initial activity. The results indicate that the fluoride and hybrid nanoflowers efficiently enhance the catalytic activity and thermal stability of fluorinase in the synthesis of 5′-fluorodeoxy adenosine, which gives a green method for producing the fluorinated organic compounds.

Suggested Citation

  • Ningning Li & Bingjing Hu & Anming Wang & Huimin Li & Youcheng Yin & Tianyu Mao & Tian Xie, 2020. "Facile Bioinspired Preparation of Fluorinase@Fluoridated Hydroxyapatite Nanoflowers for the Biosynthesis of 5′-Fluorodeoxy Adenosine," Sustainability, MDPI, vol. 12(1), pages 1-15, January.
    • Handle: RePEc:gam:jsusta:v:12:y:2020:i:1:p:431-:d:305668
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    References listed on IDEAS

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    3. Takeru Furuya & Adam S. Kamlet & Tobias Ritter, 2011. "Catalysis for fluorination and trifluoromethylation," Nature, Nature, vol. 473(7348), pages 470-477, May.
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