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First Known Report of mcr -Harboring Enterobacteriaceae in the Dominican Republic

Author

Listed:
  • Angela Perdomo

    (School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79409, USA
    These authors contributed equally to this work.)

  • Hattie E. Webb

    (Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA
    These authors contributed equally to this work.)

  • Marie Bugarel

    (Division of Research and Development Resources, BioMérieux, 69795 Lyon, France)

  • Cindy R. Friedman

    (Division of Global Migration and Quarantine, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA)

  • Louise K. Francois Watkins

    (Division of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30329, USA)

  • Guy H. Loneragan

    (School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79409, USA)

  • Alexandra Calle

    (School of Veterinary Medicine, Texas Tech University, Amarillo, TX 79409, USA)

Abstract

Colistin is a last-resort antibiotic used to treat infections caused by multidrug-resistant Gram-negative bacteria. People with a history of travel to the Dominican Republic have become sick with pathogenic bacteria carrying the mobile colistin resistance gene, mcr -1, during and after traveling. This investigation aimed to identify mcr genes in Enterobacteriaceae isolated from food animal sources in the Dominican Republic. Three hundred and eleven samples were tested, from which 1354 bacterial isolates were obtained. Real-time PCR tests showed that 70.7% (220 out of 311) of the samples and 3.2% (44 out of 1354) of the isolates tested positive for the mcr gene. All RT-PCR presumptive mcr -positive isolates (n = 44) and a subset (n = 133) of RT-PCR presumptive mcr -negative isolates were subjected to whole-genome sequencing. WGS analysis showed that 39 isolates carried the mcr gene, with 37 confirmed as positive through RT-PCR and two as negative. Further, all of the mcr -positive genomes were identified as Escherichia coli and all contained a IncX4 plasmid replicon. Resistant determinants for other antibiotics important for human health were found in almost all isolates carrying mcr genes.

Suggested Citation

  • Angela Perdomo & Hattie E. Webb & Marie Bugarel & Cindy R. Friedman & Louise K. Francois Watkins & Guy H. Loneragan & Alexandra Calle, 2023. "First Known Report of mcr -Harboring Enterobacteriaceae in the Dominican Republic," IJERPH, MDPI, vol. 20(6), pages 1-14, March.
    • Handle: RePEc:gam:jijerp:v:20:y:2023:i:6:p:5123-:d:1097106
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    References listed on IDEAS

    as
    1. Kevin Schoenmakers, 2020. "How China is getting its farmers to kick their antibiotics habit," Nature, Nature, vol. 586(7830), pages 60-62, October.
    2. S M Thumbi & M Kariuki Njenga & Thomas L Marsh & Susan Noh & Elkanah Otiang & Peninah Munyua & Linus Ochieng & Eric Ogola & Jonathan Yoder & Allan Audi & Joel M Montgomery & Godfrey Bigogo & Robert F , 2015. "Linking Human Health and Livestock Health: A “One-Health” Platform for Integrated Analysis of Human Health, Livestock Health, and Economic Welfare in Livestock Dependent Communities," PLOS ONE, Public Library of Science, vol. 10(3), pages 1-18, March.
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