IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v17y2020i14p5120-d384978.html
   My bibliography  Save this article

Evaluation of Changes in Gut Microbiota in Patients with Crohn’s Disease after Anti-Tnfα Treatment: Prospective Multicenter Observational Study

Author

Listed:
  • Laura Sanchis-Artero

    (Inflammatory Bowel Disease Unit. Department of Digestive Diseases, Hospital of Sagunto, Av. Ramón y Cajal s/n, 46520 Sagunto, Valencia, Spain)

  • Juan Francisco Martínez-Blanch

    (Genomics Laboratory. ADM-Lifesequencing. Parque Científico Universidad de Valencia. Catedrático Agustín Escardino Benlloch, 9. Edificio 2, 46980 Paterna, Valencia, Spain)

  • Sergio Manresa-Vera

    (Genomics Laboratory. ADM-Lifesequencing. Parque Científico Universidad de Valencia. Catedrático Agustín Escardino Benlloch, 9. Edificio 2, 46980 Paterna, Valencia, Spain)

  • Ernesto Cortés-Castell

    (Department of Pharmacology, Pediatrics and Organic Chemistry Miguel Hernández University, Carretera de Valencia—Alicante S/N, 03550 San Juan de Alicante, Alicante, Spain)

  • Josefa Rodriguez-Morales

    (Inflammatory Bowel Disease Unit. Department of Digestive Diseases, Hospital of Sagunto, Av. Ramón y Cajal s/n, 46520 Sagunto, Valencia, Spain)

  • Xavier Cortés-Rizo

    (Inflammatory Bowel Disease Unit. Department of Digestive Diseases, Hospital of Sagunto, Av. Ramón y Cajal s/n, 46520 Sagunto, Valencia, Spain)

Abstract

Background: Crohn’s disease is believed to result from the interaction between genetic susceptibility, environmental factors and gut microbiota, leading to an aberrant immune response. The objectives of this study are to evaluate the qualitative and quantitative changes in the microbiota of patients with Crohn’s disease after six months of anti-tumor-necrosis factor (anti-TNFα) (infliximab or adalimumab) treatment and to determine whether these changes lead to the recovery of normal microbiota when compared to a control group of healthy subjects. In addition, we will evaluate the potential role of the Faecalibacterium prausnitzii/Escherichia coli and Faecalibacterium prausnitzii/Clostridium coccoides ratios as indicators of therapeutic response to anti-TNFα drugs. Methods/Design: This prospective multicenter observational study will comprise a total of 88 subjects: 44 patients with Crohn’s disease scheduled to start anti-TNFα treatment as described in the drug specifications to control the disease and 44 healthy individuals who share the same lifestyle and eating habits. The presence of inflammatory activity will be determined by the Harvey-Bradshaw index, analytical parameters in blood, including C-reactive protein, and fecal calprotectin levels at commencement of the study, at three months and at six months, allowing the classification of patients into responders and non-responders. Microbiota composition and the quantitative relationship between Faecalibacterium prausnitzii and Escherichia coli and between Faecalibacterium prausnitzii and Clostridium coccoides group as indicators of dysbiosis will be studied at inclusion and six months after initiation of treatment using ultra sequencing with Illumina technology and comparative bioinformatics analysis for the former relationship, and digital droplet PCR using stool samples for the latter. Upon inclusion, patients will complete a survey of dietary intake for the three days prior to stool collection, which will be repeated six months later in a second collection to minimize dietary bias. Discussion: In this study, massive sequencing, a reliable new tool, will be applied to identify early biomarkers of response to anti-TNF treatment in patients with Crohn’s disease to improve clinical management of these patients, reduce morbidity rates and improve efficiency.

Suggested Citation

  • Laura Sanchis-Artero & Juan Francisco Martínez-Blanch & Sergio Manresa-Vera & Ernesto Cortés-Castell & Josefa Rodriguez-Morales & Xavier Cortés-Rizo, 2020. "Evaluation of Changes in Gut Microbiota in Patients with Crohn’s Disease after Anti-Tnfα Treatment: Prospective Multicenter Observational Study," IJERPH, MDPI, vol. 17(14), pages 1-14, July.
    • Handle: RePEc:gam:jijerp:v:17:y:2020:i:14:p:5120-:d:384978
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/17/14/5120/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/17/14/5120/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Junjie Qin & Ruiqiang Li & Jeroen Raes & Manimozhiyan Arumugam & Kristoffer Solvsten Burgdorf & Chaysavanh Manichanh & Trine Nielsen & Nicolas Pons & Florence Levenez & Takuji Yamada & Daniel R. Mende, 2010. "A human gut microbial gene catalogue established by metagenomic sequencing," Nature, Nature, vol. 464(7285), pages 59-65, March.
    2. Manimozhiyan Arumugam & Jeroen Raes & Eric Pelletier & Denis Le Paslier & Takuji Yamada & Daniel R. Mende & Gabriel R. Fernandes & Julien Tap & Thomas Bruls & Jean-Michel Batto & Marcelo Bertalan & Na, 2011. "Enterotypes of the human gut microbiome," Nature, Nature, vol. 473(7346), pages 174-180, May.
    3. Ruth E. Ley & Peter J. Turnbaugh & Samuel Klein & Jeffrey I. Gordon, 2006. "Human gut microbes associated with obesity," Nature, Nature, vol. 444(7122), pages 1022-1023, December.
    4. Manimozhiyan Arumugam & Jeroen Raes & Eric Pelletier & Denis Le Paslier & Takuji Yamada & Daniel R. Mende & Gabriel R. Fernandes & Julien Tap & Thomas Bruls & Jean-Michel Batto & Marcelo Bertalan & Na, 2011. "Erratum: Enterotypes of the human gut microbiome," Nature, Nature, vol. 474(7353), pages 666-666, June.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Diana Cardona & Pablo Roman, 2022. "New Perspectives in Health: Gut Microbiota," IJERPH, MDPI, vol. 19(10), pages 1-3, May.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Emidio Scarpellini & Emanuele Rinninella & Martina Basilico & Esther Colomier & Carlo Rasetti & Tiziana Larussa & Pierangelo Santori & Ludovico Abenavoli, 2021. "From Pre- and Probiotics to Post-Biotics: A Narrative Review," IJERPH, MDPI, vol. 19(1), pages 1-14, December.
    2. Elio L Herzog & Melania Wäfler & Irene Keller & Sebastian Wolf & Martin S Zinkernagel & Denise C Zysset-Burri, 2021. "The importance of age in compositional and functional profiling of the human intestinal microbiome," PLOS ONE, Public Library of Science, vol. 16(10), pages 1-13, October.
    3. Feng Tong & Teng Wang & Na L. Gao & Ziying Liu & Kuiqing Cui & Yiqian Duan & Sicheng Wu & Yuhong Luo & Zhipeng Li & Chengjian Yang & Yixue Xu & Bo Lin & Liguo Yang & Alfredo Pauciullo & Deshun Shi & G, 2022. "The microbiome of the buffalo digestive tract," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    4. Georges P. Schmartz & Jacqueline Rehner & Madline P. Gund & Verena Keller & Leidy-Alejandra G. Molano & Stefan Rupf & Matthias Hannig & Tim Berger & Elias Flockerzi & Berthold Seitz & Sara Fleser & Sa, 2024. "Decoding the diagnostic and therapeutic potential of microbiota using pan-body pan-disease microbiomics," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    5. Doris Vandeputte & Lindsey Commer & Raul Y. Tito & Gunter Kathagen & João Sabino & Séverine Vermeire & Karoline Faust & Jeroen Raes, 2021. "Temporal variability in quantitative human gut microbiome profiles and implications for clinical research," Nature Communications, Nature, vol. 12(1), pages 1-13, December.
    6. Antonella Gagliardi & Valentina Totino & Fatima Cacciotti & Valerio Iebba & Bruna Neroni & Giulia Bonfiglio & Maria Trancassini & Claudio Passariello & Fabrizio Pantanella & Serena Schippa, 2018. "Rebuilding the Gut Microbiota Ecosystem," IJERPH, MDPI, vol. 15(8), pages 1-24, August.
    7. Diego E. Sastre & Nazneen Sultana & Marcos V. A. S. Navarro & Maros Huliciak & Jonathan Du & Javier O. Cifuente & Maria Flowers & Xu Liu & Pete Lollar & Beatriz Trastoy & Marcelo E. Guerin & Eric J. S, 2024. "Human gut microbes express functionally distinct endoglycosidases to metabolize the same N-glycan substrate," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    8. M. C. Rühlemann & C. Bang & J. F. Gogarten & B. M. Hermes & M. Groussin & S. Waschina & M. Poyet & M. Ulrich & C. Akoua-Koffi & T. Deschner & J. J. Muyembe-Tamfum & M. M. Robbins & M. Surbeck & R. M. , 2024. "Functional host-specific adaptation of the intestinal microbiome in hominids," Nature Communications, Nature, vol. 15(1), pages 1-17, December.
    9. Zhiguang Qiu & Li Yuan & Chun-Ang Lian & Bin Lin & Jie Chen & Rong Mu & Xuejiao Qiao & Liyu Zhang & Zheng Xu & Lu Fan & Yunzeng Zhang & Shanquan Wang & Junyi Li & Huiluo Cao & Bing Li & Baowei Chen & , 2024. "BASALT refines binning from metagenomic data and increases resolution of genome-resolved metagenomic analysis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    10. Oliver Aasmets & Kertu Liis Krigul & Kreete Lüll & Andres Metspalu & Elin Org, 2022. "Gut metagenome associations with extensive digital health data in a volunteer-based Estonian microbiome cohort," Nature Communications, Nature, vol. 13(1), pages 1-11, December.
    11. Julien Tap & Franck Lejzerowicz & Aurélie Cotillard & Matthieu Pichaud & Daniel McDonald & Se Jin Song & Rob Knight & Patrick Veiga & Muriel Derrien, 2023. "Global branches and local states of the human gut microbiome define associations with environmental and intrinsic factors," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    12. Kihyun Lee & Sebastien Raguideau & Kimmo Sirén & Francesco Asnicar & Fabio Cumbo & Falk Hildebrand & Nicola Segata & Chang-Jun Cha & Christopher Quince, 2023. "Population-level impacts of antibiotic usage on the human gut microbiome," Nature Communications, Nature, vol. 14(1), pages 1-19, December.
    13. Roger S. Fujioka & Helena M. Solo-Gabriele & Muruleedhara N. Byappanahalli & Marek Kirs, 2015. "U.S. Recreational Water Quality Criteria: A Vision for the Future," IJERPH, MDPI, vol. 12(7), pages 1-25, July.
    14. Mendler, Isabella-Hilda & Drossel, Barbara & Hütt, Marc-Thorsten, 2024. "Microbiome abundance patterns as attractors and the implications for the inference of microbial interaction networks," Physica A: Statistical Mechanics and its Applications, Elsevier, vol. 639(C).
    15. Gertrude Ecklu-Mensah & Candice Choo-Kang & Maria Gjerstad Maseng & Sonya Donato & Pascal Bovet & Bharathi Viswanathan & Kweku Bedu-Addo & Jacob Plange-Rhule & Prince Oti Boateng & Terrence E. Forrest, 2023. "Gut microbiota and fecal short chain fatty acids differ with adiposity and country of origin: the METS-microbiome study," Nature Communications, Nature, vol. 14(1), pages 1-17, December.
    16. Zachary D. Wallen & Ayse Demirkan & Guy Twa & Gwendolyn Cohen & Marissa N. Dean & David G. Standaert & Timothy R. Sampson & Haydeh Payami, 2022. "Metagenomics of Parkinson’s disease implicates the gut microbiome in multiple disease mechanisms," Nature Communications, Nature, vol. 13(1), pages 1-20, December.
    17. Stefanie Widder & Lisa A. Carmody & Kristopher Opron & Linda M. Kalikin & Lindsay J. Caverly & John J. LiPuma, 2024. "Microbial community organization designates distinct pulmonary exacerbation types and predicts treatment outcome in cystic fibrosis," Nature Communications, Nature, vol. 15(1), pages 1-13, December.
    18. Courtney M. Thomas & Elie Desmond-Le Quéméner & Simonetta Gribaldo & Guillaume Borrel, 2022. "Factors shaping the abundance and diversity of the gut archaeome across the animal kingdom," Nature Communications, Nature, vol. 13(1), pages 1-16, December.
    19. Aarthi Ravikrishnan & Indrik Wijaya & Eileen Png & Kern Rei Chng & Eliza Xin Pei Ho & Amanda Hui Qi Ng & Ahmad Nazri Mohamed Naim & Jean-Sebastien Gounot & Shou Ping Guan & Jasinda Lee Hanqing & Lihua, 2024. "Gut metagenomes of Asian octogenarians reveal metabolic potential expansion and distinct microbial species associated with aging phenotypes," Nature Communications, Nature, vol. 15(1), pages 1-15, December.
    20. Zahraa Al Bander & Marloes Dekker Nitert & Aya Mousa & Negar Naderpoor, 2020. "The Gut Microbiota and Inflammation: An Overview," IJERPH, MDPI, vol. 17(20), pages 1-21, October.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:17:y:2020:i:14:p:5120-:d:384978. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.