IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v16y2019i12p2205-d242049.html
   My bibliography  Save this article

Characterization and Phylogenetic Analysis of Campylobacter Species Isolated from Paediatric Stool and Water Samples in the Northwest Province, South Africa

Author

Listed:
  • Martina O. Chukwu

    (Department of Life and Consumer science, College of Agriculture and Environmental Sciences, University of South Africa, Corner Christiaan De wet and Pioneer Avenue, 1724 Florida park Roodepoort, Gauteng 1709, South Africa)

  • Akebe Luther King Abia

    (Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa)

  • Eunice Ubomba-Jaswa

    (Department of Biotechnology, University of Johannesburg, 37 Nind Street, Doornfontein, Gauteng 2094, South Africa
    Water Research Commission, Lynnwood Bridge Office Park, Bloukrans Building, 4 Daventry Street, Lynnwood Manor, Pretoria 0081, South Africa)

  • Lawrence Obi

    (Sefako Makgatho Health Science University, Molotlegi Street, Ga-Rankuwa, Pretoria, Gauteng, P.O Box 60, Medunsa 0204, South Africa)

  • John Barr Dewar

    (Department of Life and Consumer science, College of Agriculture and Environmental Sciences, University of South Africa, Corner Christiaan De wet and Pioneer Avenue, 1724 Florida park Roodepoort, Gauteng 1709, South Africa)

Abstract

Antibiotic-resistant Campylobacter could adversely affect treatment outcomes, especially in children. We investigated the antibiotic susceptibility profiles, virulence potentials and genetic relatedness of Campylobacter spp. from paediatric and water samples in the North West Province, South Africa. Overall, 237 human and 20 water isolates were identified using culture and real-time polymerase chain reaction (PCR). The antibiotic susceptibility profiles were determined using the disk diffusion method. Gradient strips were used to determine the minimum inhibitory concentration of each antibiotic. Antibiotic resistance ( gryA , tetO and 23S rRNA 2075G and 2074C) and virulence ( cadF and ciaB ) genes were also investigated using PCR. A phylogenetic tree to ascertain the clonality between water and clinical isolates was constructed using MEGA 7. Overall, 95% (water) and 64.7% (human) of the isolates were resistant to at least one antibiotic tested. The highest resistance was against clarithromycin (95%) for water and ampicillin (60.7%) for human isolates. The 23S rRNA 2075G/2074C mutation was the most expressed resistance gene. Phylogenetic reconstruction revealed eight intermixed clades within water and human Campylobacter isolates. This study suggests the possible circulation of potentially pathogenic antibiotic-resistant Campylobacter in the Northwest Province, South Africa with drinking water being a possible vector for disease transmission in this area.

Suggested Citation

  • Martina O. Chukwu & Akebe Luther King Abia & Eunice Ubomba-Jaswa & Lawrence Obi & John Barr Dewar, 2019. "Characterization and Phylogenetic Analysis of Campylobacter Species Isolated from Paediatric Stool and Water Samples in the Northwest Province, South Africa," IJERPH, MDPI, vol. 16(12), pages 1-22, June.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:12:p:2205-:d:242049
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/16/12/2205/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/16/12/2205/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Agnieszka Chlebicz & Katarzyna Śliżewska, 2018. "Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review," IJERPH, MDPI, vol. 15(5), pages 1-28, April.
    2. Akebe Luther King Abia & Lisa Schaefer & Eunice Ubomba-Jaswa & Wouter Le Roux, 2017. "Abundance of Pathogenic Escherichia coli Virulence-Associated Genes in Well and Borehole Water Used for Domestic Purposes in a Peri-Urban Community of South Africa," IJERPH, MDPI, vol. 14(3), pages 1-11, March.
    3. Anthony C. Otigbu & Anna M. Clarke & Justine Fri & Emmanuel O. Akanbi & Henry A. Njom, 2018. "Antibiotic Sensitivity Profiling and Virulence Potential of Campylobacter jejuni Isolates from Estuarine Water in the Eastern Cape Province, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-15, May.
    4. Mokaba Shirley Malema & Akebe Luther King Abia & Roman Tandlich & Bonga Zuma & Jean-Marc Mwenge Kahinda & Eunice Ubomba-Jaswa, 2018. "Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-14, May.
    5. María Ugarte-Ruiz & Diego Florez-Cuadrado & Trudy M. Wassenaar & María Concepción Porrero & Lucas Domínguez, 2015. "Method Comparison for Enhanced Recovery, Isolation and Qualitative Detection of C. jejuni and C. coli from Wastewater Effluent Samples," IJERPH, MDPI, vol. 12(3), pages 1-16, March.
    6. J. Parkhill & B. W. Wren & K. Mungall & J. M. Ketley & C. Churcher & D. Basham & T. Chillingworth & R. M. Davies & T. Feltwell & S. Holroyd & K. Jagels & A. V. Karlyshev & S. Moule & M. J. Pallen & C., 2000. "The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences," Nature, Nature, vol. 403(6770), pages 665-668, February.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Adele Evans & Anthony J. Slate & I. Devine Akhidime & Joanna Verran & Peter J. Kelly & Kathryn A. Whitehead, 2021. "The Removal of Meat Exudate and Escherichia coli from Stainless Steel and Titanium Surfaces with Irregular and Regular Linear Topographies," IJERPH, MDPI, vol. 18(6), pages 1-16, March.
    2. Thandi Kapwata & Angela Mathee & Wouter Jacobus Le Roux & Caradee Yael Wright, 2018. "Diarrhoeal Disease in Relation to Possible Household Risk Factors in South African Villages," IJERPH, MDPI, vol. 15(8), pages 1-12, August.
    3. Constantine-Richard Stefanou & Anna Szosland-Fałtyn & Beata Bartodziejska, 2023. "Survey of Domestic Refrigerator Storage Temperatures in Poland for Use as a QMRA Tool for Exposure Assessment," IJERPH, MDPI, vol. 20(4), pages 1-9, February.
    4. Dorit Nitzan & Bindu Nishal Andreuzza & Deepanwita Chattopadhyay, 2023. "The Food Systems, One Health, and Resilience (FOR) Approach—Led by the FOR-Runners," Sustainability, MDPI, vol. 15(18), pages 1-13, September.
    5. Mohora Feida Malebatja & Mpata Mathildah Mokgatle, 2022. "Diarrhoea among Children Aged 5 Years and Microbial Drinking Water Quality Compliance: Trends Analysis Study in South Africa (2008–2018)," IJERPH, MDPI, vol. 20(1), pages 1-14, December.
    6. Nicol Strakova & Kristyna Korena & Tereza Gelbicova & Pavel Kulich & Renata Karpiskova, 2021. "A Rapid Culture Method for the Detection of Campylobacter from Water Environments," IJERPH, MDPI, vol. 18(11), pages 1-13, June.
    7. Marcin Pigłowski, 2019. "Pathogenic and Non-Pathogenic Microorganisms in the Rapid Alert System for Food and Feed," IJERPH, MDPI, vol. 16(3), pages 1-19, February.
    8. Kousar Banu Hoorzook & Anton Pieterse & Lee Heine & Tobias George Barnard & Nickey Janse van Rensburg, 2021. "Soul of the Jukskei River: The Extent of Bacterial Contamination in the Jukskei River in Gauteng Province, South Africa," IJERPH, MDPI, vol. 18(16), pages 1-19, August.
    9. Martin Ackermann & Lin Chao, 2006. "DNA Sequences Shaped by Selection for Stability," PLOS Genetics, Public Library of Science, vol. 2(2), pages 1-7, February.
    10. Christopher Ruis & Aaron Weimann & Gerry Tonkin-Hill & Arun Prasad Pandurangan & Marta Matuszewska & Gemma G. R. Murray & Roger C. Lévesque & Tom L. Blundell & R. Andres Floto & Julian Parkhill, 2023. "Mutational spectra are associated with bacterial niche," Nature Communications, Nature, vol. 14(1), pages 1-11, December.
    11. Tsolanku Sidney Maliehe & Nelisiwe Mavingo & Tlou Nelson Selepe & Peter Masoko & Frederick Mokibelo Mashao & Neville Nyamutswa, 2024. "Quantitative Assessment of Human Health Risks Associated with Heavy Metal and Bacterial Pollution in Groundwater from Mankweng in Limpopo Province, South Africa," IJERPH, MDPI, vol. 21(11), pages 1-19, November.
    12. Fabian König & Sarah L. Svensson & Cynthia M. Sharma, 2024. "Interplay of two small RNAs fine-tunes hierarchical flagella gene expression in Campylobacter jejuni," Nature Communications, Nature, vol. 15(1), pages 1-21, December.
    13. Ashok J. Tamhankar & Cecilia Stålsby Lundborg, 2019. "Antimicrobials and Antimicrobial Resistance in the Environment and Its Remediation: A Global One Health Perspective," IJERPH, MDPI, vol. 16(23), pages 1-7, November.
    14. Sabina Fijan & Dunja Šulc & Andrej Steyer, 2018. "Study of the In Vitro Antagonistic Activity of Various Single-Strain and Multi-Strain Probiotics against Escherichia coli," IJERPH, MDPI, vol. 15(7), pages 1-15, July.
    15. Conrado Carrascosa & Dele Raheem & Fernando Ramos & Ariana Saraiva & António Raposo, 2021. "Microbial Biofilms in the Food Industry—A Comprehensive Review," IJERPH, MDPI, vol. 18(4), pages 1-31, February.
    16. Mokaba Shirley Malema & Akebe Luther King Abia & Roman Tandlich & Bonga Zuma & Jean-Marc Mwenge Kahinda & Eunice Ubomba-Jaswa, 2018. "Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-14, May.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:16:y:2019:i:12:p:2205-:d:242049. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.