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Characterization and Phylogenetic Analysis of Campylobacter Species Isolated from Paediatric Stool and Water Samples in the Northwest Province, South Africa

Author

Listed:
  • Martina O. Chukwu

    (Department of Life and Consumer science, College of Agriculture and Environmental Sciences, University of South Africa, Corner Christiaan De wet and Pioneer Avenue, 1724 Florida park Roodepoort, Gauteng 1709, South Africa)

  • Akebe Luther King Abia

    (Antimicrobial Research Unit, College of Health Sciences, University of KwaZulu-Natal, Private Bag X54001, Durban 4000, South Africa)

  • Eunice Ubomba-Jaswa

    (Department of Biotechnology, University of Johannesburg, 37 Nind Street, Doornfontein, Gauteng 2094, South Africa
    Water Research Commission, Lynnwood Bridge Office Park, Bloukrans Building, 4 Daventry Street, Lynnwood Manor, Pretoria 0081, South Africa)

  • Lawrence Obi

    (Sefako Makgatho Health Science University, Molotlegi Street, Ga-Rankuwa, Pretoria, Gauteng, P.O Box 60, Medunsa 0204, South Africa)

  • John Barr Dewar

    (Department of Life and Consumer science, College of Agriculture and Environmental Sciences, University of South Africa, Corner Christiaan De wet and Pioneer Avenue, 1724 Florida park Roodepoort, Gauteng 1709, South Africa)

Abstract

Antibiotic-resistant Campylobacter could adversely affect treatment outcomes, especially in children. We investigated the antibiotic susceptibility profiles, virulence potentials and genetic relatedness of Campylobacter spp. from paediatric and water samples in the North West Province, South Africa. Overall, 237 human and 20 water isolates were identified using culture and real-time polymerase chain reaction (PCR). The antibiotic susceptibility profiles were determined using the disk diffusion method. Gradient strips were used to determine the minimum inhibitory concentration of each antibiotic. Antibiotic resistance ( gryA , tetO and 23S rRNA 2075G and 2074C) and virulence ( cadF and ciaB ) genes were also investigated using PCR. A phylogenetic tree to ascertain the clonality between water and clinical isolates was constructed using MEGA 7. Overall, 95% (water) and 64.7% (human) of the isolates were resistant to at least one antibiotic tested. The highest resistance was against clarithromycin (95%) for water and ampicillin (60.7%) for human isolates. The 23S rRNA 2075G/2074C mutation was the most expressed resistance gene. Phylogenetic reconstruction revealed eight intermixed clades within water and human Campylobacter isolates. This study suggests the possible circulation of potentially pathogenic antibiotic-resistant Campylobacter in the Northwest Province, South Africa with drinking water being a possible vector for disease transmission in this area.

Suggested Citation

  • Martina O. Chukwu & Akebe Luther King Abia & Eunice Ubomba-Jaswa & Lawrence Obi & John Barr Dewar, 2019. "Characterization and Phylogenetic Analysis of Campylobacter Species Isolated from Paediatric Stool and Water Samples in the Northwest Province, South Africa," IJERPH, MDPI, vol. 16(12), pages 1-22, June.
    • Handle: RePEc:gam:jijerp:v:16:y:2019:i:12:p:2205-:d:242049
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    References listed on IDEAS

    as
    1. Agnieszka Chlebicz & Katarzyna Śliżewska, 2018. "Campylobacteriosis, Salmonellosis, Yersiniosis, and Listeriosis as Zoonotic Foodborne Diseases: A Review," IJERPH, MDPI, vol. 15(5), pages 1-28, April.
    2. J. Parkhill & B. W. Wren & K. Mungall & J. M. Ketley & C. Churcher & D. Basham & T. Chillingworth & R. M. Davies & T. Feltwell & S. Holroyd & K. Jagels & A. V. Karlyshev & S. Moule & M. J. Pallen & C., 2000. "The genome sequence of the food-borne pathogen Campylobacter jejuni reveals hypervariable sequences," Nature, Nature, vol. 403(6770), pages 665-668, February.
    3. Akebe Luther King Abia & Lisa Schaefer & Eunice Ubomba-Jaswa & Wouter Le Roux, 2017. "Abundance of Pathogenic Escherichia coli Virulence-Associated Genes in Well and Borehole Water Used for Domestic Purposes in a Peri-Urban Community of South Africa," IJERPH, MDPI, vol. 14(3), pages 1-11, March.
    4. Anthony C. Otigbu & Anna M. Clarke & Justine Fri & Emmanuel O. Akanbi & Henry A. Njom, 2018. "Antibiotic Sensitivity Profiling and Virulence Potential of Campylobacter jejuni Isolates from Estuarine Water in the Eastern Cape Province, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-15, May.
    5. Mokaba Shirley Malema & Akebe Luther King Abia & Roman Tandlich & Bonga Zuma & Jean-Marc Mwenge Kahinda & Eunice Ubomba-Jaswa, 2018. "Antibiotic-Resistant Pathogenic Escherichia Coli Isolated from Rooftop Rainwater-Harvesting Tanks in the Eastern Cape, South Africa," IJERPH, MDPI, vol. 15(5), pages 1-14, May.
    6. María Ugarte-Ruiz & Diego Florez-Cuadrado & Trudy M. Wassenaar & María Concepción Porrero & Lucas Domínguez, 2015. "Method Comparison for Enhanced Recovery, Isolation and Qualitative Detection of C. jejuni and C. coli from Wastewater Effluent Samples," IJERPH, MDPI, vol. 12(3), pages 1-16, March.
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