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Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations

Author

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  • Krzysztof Poszytek

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Joanna Karczewska-Golec

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Anna Ciok

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Przemyslaw Decewicz

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Mikolaj Dziurzynski

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Adrian Gorecki

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Grazyna Jakusz

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Tomasz Krucon

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Pola Lomza

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Krzysztof Romaniuk

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Michal Styczynski

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Zhendong Yang

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Lukasz Drewniak

    (Laboratory of Environmental Pollution Analysis, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

  • Lukasz Dziewit

    (Department of Bacterial Genetics, Institute of Microbiology, Faculty of Biology, University of Warsaw, Miecznikowa 1, 02-096 Warsaw, Poland)

Abstract

Sewage sludge is an abundant source of microorganisms that are metabolically active against numerous contaminants, and thus possibly useful in environmental biotechnologies. However, amongst the sewage sludge isolates, pathogenic bacteria can potentially be found, and such isolates should therefore be carefully tested before their application. A novel bacterial strain, Ochrobactrum sp. POC9, was isolated from a sewage sludge sample collected from a wastewater treatment plant. The strain exhibited lipolytic, proteolytic, cellulolytic, and amylolytic activities, which supports its application in biodegradation of complex organic compounds. We demonstrated that bioaugmentation with this strain substantially improved the overall biogas production and methane content during anaerobic digestion of sewage sludge. The POC9 genome content analysis provided a deeper insight into the biotechnological potential of this bacterium and revealed that it is a metalotolerant and a biofilm-producing strain capable of utilizing various toxic compounds. The strain is resistant to rifampicin, chloramphenicol and β-lactams. The corresponding antibiotic resistance genes (including bla OCH and cmlA/floR ) were identified in the POC9 genome. Nevertheless, as only few genes in the POC9 genome might be linked to pathogenicity, and none of those genes is a critical virulence factor found in severe pathogens, the strain appears safe for application in environmental biotechnologies.

Suggested Citation

  • Krzysztof Poszytek & Joanna Karczewska-Golec & Anna Ciok & Przemyslaw Decewicz & Mikolaj Dziurzynski & Adrian Gorecki & Grazyna Jakusz & Tomasz Krucon & Pola Lomza & Krzysztof Romaniuk & Michal Styczy, 2018. "Genome-Guided Characterization of Ochrobactrum sp. POC9 Enhancing Sewage Sludge Utilization—Biotechnological Potential and Biosafety Considerations," IJERPH, MDPI, vol. 15(7), pages 1-17, July.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:7:p:1501-:d:158251
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    References listed on IDEAS

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    1. Werle, Sebastian & Wilk, Ryszard K., 2010. "A review of methods for the thermal utilization of sewage sludge: The Polish perspective," Renewable Energy, Elsevier, vol. 35(9), pages 1914-1919.
    2. Ariunbaatar, Javkhlan & Panico, Antonio & Esposito, Giovanni & Pirozzi, Francesco & Lens, Piet N.L., 2014. "Pretreatment methods to enhance anaerobic digestion of organic solid waste," Applied Energy, Elsevier, vol. 123(C), pages 143-156.
    3. Ruchita Dixit & Wasiullah & Deepti Malaviya & Kuppusamy Pandiyan & Udai B. Singh & Asha Sahu & Renu Shukla & Bhanu P. Singh & Jai P. Rai & Pawan Kumar Sharma & Harshad Lade & Diby Paul, 2015. "Bioremediation of Heavy Metals from Soil and Aquatic Environment: An Overview of Principles and Criteria of Fundamental Processes," Sustainability, MDPI, vol. 7(2), pages 1-24, February.
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    1. Amanda Pacholak & Wojciech Smułek & Agnieszka Zgoła-Grześkowiak & Ewa Kaczorek, 2019. "Nitrofurantoin—Microbial Degradation and Interactions with Environmental Bacterial Strains," IJERPH, MDPI, vol. 16(9), pages 1-15, April.
    2. Abdullah Kaviani Rad & Angelika Astaykina & Rostislav Streletskii & Yeganeh Afsharyzad & Hassan Etesami & Mehdi Zarei & Siva K. Balasundram, 2022. "An Overview of Antibiotic Resistance and Abiotic Stresses Affecting Antimicrobial Resistance in Agricultural Soils," IJERPH, MDPI, vol. 19(8), pages 1-27, April.
    3. Magdalena Lebiocka & Agnieszka Montusiewicz & Agnieszka Cydzik-Kwiatkowska, 2018. "Effect of Bioaugmentation on Biogas Yields and Kinetics in Anaerobic Digestion of Sewage Sludge," IJERPH, MDPI, vol. 15(8), pages 1-16, August.
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