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Activation of the Nrf2/HO-1 Signaling Pathway Contributes to the Protective Effects of Sargassum serratifolium Extract against Oxidative Stress-Induced DNA Damage and Apoptosis in SW1353 Human Chondrocytes

Author

Listed:
  • Cheol Park

    (Department of Molecular Biology, College of Natural Sciences, Dongeui University, Busan 47340, Korea)

  • Su Hyun Hong

    (Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 47227, Korea
    Anti-Aging Research Center, Dongeui University, Busan 47340, Korea)

  • Soon Shik Shin

    (Department of Formula Sciences, Dongeui University College of Korean Medicine, Busan 47227, Korea)

  • Dae-Sung Lee

    (National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea)

  • Min Ho Han

    (National Marine Biodiversity Institute of Korea, Seocheon 33662, Korea)

  • Hee-Jae Cha

    (Department of Parasitology and Genetics, Kosin University College of Medicine, Busan 49267, Korea)

  • Suhkmann Kim

    (Department of Chemistry, College of Natural Sciences, Center for Proteome Biophysics and Chemistry Institute for Functional Materials, Pusan National University, Busan 46241, Korea)

  • Heui-Soo Kim

    (Department of Biological Sciences, College of Natural Sciences, Pusan National University, Busan 46241, Korea)

  • Gi-Young Kim

    (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea)

  • Eui Kyun Park

    (Department of Oral Pathology and Regenerative Medicine, School of Dentistry, Institute for Hard Tissue and Biotooth Regeneration, Kyungpook National University, Daegu 41940, Korea)

  • You-Jin Jeon

    (Department of Marine Life Sciences, Jeju National University, Jeju 63243, Korea)

  • Yung Hyun Choi

    (Department of Biochemistry, Dongeui University College of Korean Medicine, Busan 47227, Korea
    Anti-Aging Research Center, Dongeui University, Busan 47340, Korea)

Abstract

Oxidative stress in chondrocytes plays a critical role in the pathogenesis of osteoarthritis as an important cause of articular cartilage degradation. Sargassum serratifolium C. Agardh, a marine brown algae, is known to have potent antioxidant activity. Nevertheless, no study has been conducted yet on the protective efficacy against oxidative stress in chondrocytes. Therefore, the aim of the current study is to investigate the mechanism of the antioxidative effect of ethanol extract of S. serratifolium (EESS) on DNA damage and apoptosis induced by hydrogen peroxide (H 2 O 2 ) in SW1353 human chondrocytes. For this purpose, SW1353 cells exposed to H 2 O 2 in the presence or absence of EESS were applied to cell viability assay, comet assay, immunoblotting and flow cytometry analyses. Our results showed that EESS effectively attenuated H 2 O 2 -induced cytotoxicity and DNA damage associated with the inhibition of reactive oxygen species (ROS) accumulation. EESS also weakened the mitochondria membrane permeabilization by H 2 O 2 , and recovered H 2 O 2 -induced decreased expression of anti-apoptotic Bcl-2 and pro-caspase-3, and degradation of poly (ADP-ribose) polymerase. In addition, EESS increased not only expression, but also phosphorylation of nuclear factor-erythroid 2 related factor 2 (Nrf2), and promoted the expression of heme oxygenase-1 (HO-1), a critical target enzyme of Nrf2, but decreased the expression of kelch-like ECH-associated protein-1; however, the inhibition of HO-1 activity by zinc protoporphyrin abolished the antioxidant potential induced by EESS against H 2 O 2 -mediated oxidative stress. Therefore, the results of this study suggest that the antioxidant efficacy of EESS in chondrocytes is at least involved in the Nrf2/HO-1 signaling pathway-dependent mechanism.

Suggested Citation

  • Cheol Park & Su Hyun Hong & Soon Shik Shin & Dae-Sung Lee & Min Ho Han & Hee-Jae Cha & Suhkmann Kim & Heui-Soo Kim & Gi-Young Kim & Eui Kyun Park & You-Jin Jeon & Yung Hyun Choi, 2018. "Activation of the Nrf2/HO-1 Signaling Pathway Contributes to the Protective Effects of Sargassum serratifolium Extract against Oxidative Stress-Induced DNA Damage and Apoptosis in SW1353 Human Chondro," IJERPH, MDPI, vol. 15(6), pages 1-13, June.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:6:p:1173-:d:150658
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    References listed on IDEAS

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    1. Toren Finkel & Nikki J. Holbrook, 2000. "Oxidants, oxidative stress and the biology of ageing," Nature, Nature, vol. 408(6809), pages 239-247, November.
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    1. Cheol Park & Da Hye Kwon & Su Jung Hwang & Min Ho Han & Jin-Woo Jeong & Sang Hoon Hong & Hee-Jae Cha & Su-Hyun Hong & Gi-Young Kim & Hyo-Jong Lee & Suhkmann Kim & Heui-Soo Kim & Yung Hyun Choi, 2019. "Protective Effects of Nargenicin A1 against Tacrolimus-Induced Oxidative Stress in Hirame Natural Embryo Cells," IJERPH, MDPI, vol. 16(6), pages 1-13, March.

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