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Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches

Author

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  • Meiqing Zhu

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Lijun Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yu Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Jie Zhou

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Jie Ding

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Wei Li

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yue Xin

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Shisuo Fan

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Zhen Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yi Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

Abstract

The herbicide atrazine is widely used across the globe, which is a great concern. To investigate its potential toxicity in the human body, human serum albumin (HSA) was selected as a model protein. The interaction between atrazine and HSA was investigated using steady-state fluorescence spectroscopy, synchronous fluorescence spectroscopy, UV-Vis spectroscopy, three-dimensional (3D) fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The intrinsic fluorescence of HSA was quenched by the atrazine through a static quenching mechanism. Fluorescence spectra at two excitation wavelengths (280 and 295 nm) showed that the fluorescence quenched in HSA was mainly contributed to by tryptophan residues. In addition, the atrazine bound to HSA, which induced changes in the conformation and secondary structure of HSA and caused an energy transfer. Thermodynamic parameters revealed that this binding is spontaneous. Moreover, electrostatic interactions play a major role in the combination of atrazine and HSA. One atrazine molecule can only bind to one HSA molecule to form a complex, and the atrazine molecule is bound at site II (subdomain IIIA) of HSA. This study furthers the understanding of the potential effects posed by atrazine on humans at the molecular level.

Suggested Citation

  • Meiqing Zhu & Lijun Wang & Yu Wang & Jie Zhou & Jie Ding & Wei Li & Yue Xin & Shisuo Fan & Zhen Wang & Yi Wang, 2018. "Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches," IJERPH, MDPI, vol. 15(1), pages 1-16, January.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:1:p:116-:d:126525
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    References listed on IDEAS

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    1. Dong Xu & Qingyun Wang & Tao Yang & Jianzhong Cao & Qinlu Lin & Zhiqin Yuan & Le Li, 2016. "Polyethyleneimine Capped Silver Nanoclusters as Efficient Antibacterial Agents," IJERPH, MDPI, vol. 13(3), pages 1-11, March.
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    4. Ruihong Yang & Jianzhong ZHU & Yingliu Li & Hui Zhang, 2016. "A Study on the Preparation of Regular Multiple Micro-Electrolysis Filler and the Application in Pretreatment of Oil Refinery Wastewater," IJERPH, MDPI, vol. 13(5), pages 1-15, April.
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    Cited by:

    1. Hans-Peter Hutter & Hanns Moshammer, 2018. "Pesticides Are an Occupational and Public Health Issue," IJERPH, MDPI, vol. 15(8), pages 1-3, August.

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