IDEAS home Printed from https://ideas.repec.org/a/gam/jijerp/v15y2018i1p116-d126525.html
   My bibliography  Save this article

Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches

Author

Listed:
  • Meiqing Zhu

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Lijun Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yu Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Jie Zhou

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Jie Ding

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Wei Li

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yue Xin

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Shisuo Fan

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Zhen Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

  • Yi Wang

    (Key Laboratory of Agri-Food Safety of Anhui Province, School of Resources and Environment, Anhui Agricultural University, Hefei 230036, China)

Abstract

The herbicide atrazine is widely used across the globe, which is a great concern. To investigate its potential toxicity in the human body, human serum albumin (HSA) was selected as a model protein. The interaction between atrazine and HSA was investigated using steady-state fluorescence spectroscopy, synchronous fluorescence spectroscopy, UV-Vis spectroscopy, three-dimensional (3D) fluorescence spectroscopy and circular dichroism (CD) spectroscopy. The intrinsic fluorescence of HSA was quenched by the atrazine through a static quenching mechanism. Fluorescence spectra at two excitation wavelengths (280 and 295 nm) showed that the fluorescence quenched in HSA was mainly contributed to by tryptophan residues. In addition, the atrazine bound to HSA, which induced changes in the conformation and secondary structure of HSA and caused an energy transfer. Thermodynamic parameters revealed that this binding is spontaneous. Moreover, electrostatic interactions play a major role in the combination of atrazine and HSA. One atrazine molecule can only bind to one HSA molecule to form a complex, and the atrazine molecule is bound at site II (subdomain IIIA) of HSA. This study furthers the understanding of the potential effects posed by atrazine on humans at the molecular level.

Suggested Citation

  • Meiqing Zhu & Lijun Wang & Yu Wang & Jie Zhou & Jie Ding & Wei Li & Yue Xin & Shisuo Fan & Zhen Wang & Yi Wang, 2018. "Biointeractions of Herbicide Atrazine with Human Serum Albumin: UV-Vis, Fluorescence and Circular Dichroism Approaches," IJERPH, MDPI, vol. 15(1), pages 1-16, January.
    • Handle: RePEc:gam:jijerp:v:15:y:2018:i:1:p:116-:d:126525
    as

    Download full text from publisher

    File URL: https://www.mdpi.com/1660-4601/15/1/116/pdf
    Download Restriction: no
    File URL: https://www.mdpi.com/1660-4601/15/1/116/
    Download Restriction: no
    ---><---

    References listed on IDEAS

    as
    1. Jihong Dong & Wenting Dai & Jiren Xu & Songnian Li, 2016. "Spectral Estimation Model Construction of Heavy Metals in Mining Reclamation Areas," IJERPH, MDPI, vol. 13(7), pages 1-18, June.
    2. Dong Xu & Qingyun Wang & Tao Yang & Jianzhong Cao & Qinlu Lin & Zhiqin Yuan & Le Li, 2016. "Polyethyleneimine Capped Silver Nanoclusters as Efficient Antibacterial Agents," IJERPH, MDPI, vol. 13(3), pages 1-11, March.
    3. Jan Kühnisch & Inka Goddon & Susanne Berger & Helga Senkel & Katharina Bücher & Thomas Oehme & Reinhard Hickel & Roswitha Heinrich-Weltzien, 2009. "Development, Methodology and Potential of the New Universal Visual Scoring System (UniViSS) for Caries Detection and Diagnosis," IJERPH, MDPI, vol. 6(9), pages 1-10, September.
    4. Xiaoxia Zheng & Wenji Zhao & Xing Yan & Tongtong Shu & Qiulin Xiong & Fantao Chen, 2015. "Pollution Characteristics and Health Risk Assessment of Airborne Heavy Metals Collected from Beijing Bus Stations," IJERPH, MDPI, vol. 12(8), pages 1-14, August.
    5. Ruihong Yang & Jianzhong ZHU & Yingliu Li & Hui Zhang, 2016. "A Study on the Preparation of Regular Multiple Micro-Electrolysis Filler and the Application in Pretreatment of Oil Refinery Wastewater," IJERPH, MDPI, vol. 13(5), pages 1-15, April.
    6. Liang Sun & Yan Li & Aimin Li, 2015. "Treatment of Actual Chemical Wastewater by a Heterogeneous Fenton Process Using Natural Pyrite," IJERPH, MDPI, vol. 12(11), pages 1-17, October.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Hans-Peter Hutter & Hanns Moshammer, 2018. "Pesticides Are an Occupational and Public Health Issue," IJERPH, MDPI, vol. 15(8), pages 1-3, August.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Bassam Tawabini & Mubarak Al-Enazi & Mansour A. Alghamdi & Ashraf Farahat & Ahsan M. Shemsi & Marwan Y. Al Sharif & Mamdouh I. Khoder, 2023. "Potentially Harmful Elements Associated with Dust of Mosques: Pollution Status, Sources, and Human Health Risks," IJERPH, MDPI, vol. 20(3), pages 1-30, February.
    2. Jiu Huang & Peng Wang & Chaorong Xu & Zhuangzhuang Zhu, 2018. "Fly Ash Modified Coalmine Solid Wastes for Stabilization of Trace Metals in Mining Damaged Land Reclamation: A Case Study in Xuzhou Coalmine Area," IJERPH, MDPI, vol. 15(10), pages 1-23, October.
    3. Qu, Baolin & Zhu, Hongqing & Tian, Rui & Hu, Lintao & Wang, Jingxin & Liao, Qi & Gao, Rongxiang & Wang, Haoran, 2023. "Investigation of the impact of pyrite content on the terahertz dielectric response of coals and rapid recognition with kernel-SVM," Energy, Elsevier, vol. 285(C).
    4. Jaeseok Heo & Yelim Jang & Michael Versoza & Gihwan Kim & Duckshin Park, 2021. "A New Method of Removing Fine Particulates Using an Electrostatic Force," IJERPH, MDPI, vol. 18(12), pages 1-10, June.
    5. Yanfeng Zhu & Jing Ma & Fu Chen & Ruilian Yu & Gongren Hu & Shaoliang Zhang, 2020. "Remediation of Soil Polluted with Cd in a Postmining Area Using Thiourea-Modified Biochar," IJERPH, MDPI, vol. 17(20), pages 1-13, October.
    6. Liang Sun & Dehao Hu & Ziyu Zhang & Xiaoyan Deng, 2019. "Oxidative Degradation of Methylene Blue via PDS-Based Advanced Oxidation Process Using Natural Pyrite," IJERPH, MDPI, vol. 16(23), pages 1-12, November.
    7. Dejun Wan & Changlin Zhan & Guanglin Yang & Xingqi Liu & Jinsong Yang, 2016. "Preliminary Assessment of Health Risks of Potentially Toxic Elements in Settled Dust over Beijing Urban Area," IJERPH, MDPI, vol. 13(5), pages 1-15, May.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:gam:jijerp:v:15:y:2018:i:1:p:116-:d:126525. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: MDPI Indexing Manager (email available below). General contact details of provider: https://www.mdpi.com .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.