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Analysis of the Toll-Like Receptor 2-2 (TLR2-2) and TLR4 mRNA Expression in the Intestinal Mucosal Immunity of Broilers Fed on Diets Supplemented with Nickel Chloride

Author

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  • Bangyuan Wu

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Hengmin Cui

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Xi Peng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Jing Fang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Zhicai Zuo

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Junliang Deng

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

  • Jianying Huang

    (Key Laboratory of Animal Diseases and Environmental Hazards of Sichuan Province, College of Veterinary Medicine, Sichuan Agricultural University, Ya'an 625014, China)

Abstract

Toll-like receptor (TLRs) are important innate immune receptors, and TLR2 and TLR4 play an important role in intestinal mucosal innate immunity. It has been found that nickel (Ni) can affect the immune system in broilers. The purpose of this study was to analyze changes in TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosal immunity system of broilers induced by dietary nickel chloride (NiCl 2 ) using quantitative real-time polymerase chain reaction (qRT-PCR) assays. Two hundred and forty one-day-old avian broilers were divided into four groups and fed on a corn-soybean basal diet as control diet or the same basal diet supplemented with 300, 600 and 900 mg/kg of NiCl 2 for 42 days. Results showed that the TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and the cecal tonsil were lower ( p < 0.05 or p < 0.01) in the 300, 600 and 900 mg/kg groups than those in the control group. It was concluded that dietary NiCl 2 in excess of 300 mg/kg could reduce TLR2-2 and TLR4 mRNA expression levels in the intestinal mucosa and cecal tonsil in broilers, implying that the innate immunity in intestinal mucosal immune system could be impaired by pathways involving injured surface epithelium cells or/and the inhibition of the TLR signal transduction.

Suggested Citation

  • Bangyuan Wu & Hengmin Cui & Xi Peng & Jing Fang & Zhicai Zuo & Junliang Deng & Jianying Huang, 2014. "Analysis of the Toll-Like Receptor 2-2 (TLR2-2) and TLR4 mRNA Expression in the Intestinal Mucosal Immunity of Broilers Fed on Diets Supplemented with Nickel Chloride," IJERPH, MDPI, vol. 11(1), pages 1-14, January.
    • Handle: RePEc:gam:jijerp:v:11:y:2014:i:1:p:657-670:d:31842
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    References listed on IDEAS

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    1. Jacques Banchereau & Ralph M. Steinman, 1998. "Dendritic cells and the control of immunity," Nature, Nature, vol. 392(6673), pages 245-252, March.
    2. Richard J. Ulevitch, 1999. "Toll gates for pathogen selection," Nature, Nature, vol. 401(6755), pages 755-756, October.
    3. Richard J. Ulevitch, 1999. "Toll gates for pathogen selection," Nature, Nature, vol. 402(6763), pages 5-5, December.
    4. Bangyuan Wu & Hengmin Cui & Xi Peng & Jing Fang & Zhicai Zuo & Junliang Deng & Jianying Huang, 2013. "Dietary Nickel Chloride Induces Oxidative Intestinal Damage in Broilers," IJERPH, MDPI, vol. 10(6), pages 1-11, May.
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