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Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity

Author

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  • Sigita Vaičiulytė

    (Department of Botany and Genetics, Faculty of Natural Sciences, Vilnius University, M.K.Ciurlionio 21/27, Vilnius LT-03101, Lithuania
    Department of Chemical and Biological Engineering, Division of Industrial Biotechnology, Chalmers University of Technology, Kemivägen 10, Gothenburg SE-412 96, Sweden)

  • Giulia Padovani

    (Institute of Ecosystem Study, National Research Council (CNR), Section of Florence, Polo Scientifico, Via Madonna del Piano n. 10, Sesto Fiorentino (FI) IT-50019, Italy)

  • Jolanta Kostkevičienė

    (Department of Botany and Genetics, Faculty of Natural Sciences, Vilnius University, M.K.Ciurlionio 21/27, Vilnius LT-03101, Lithuania)

  • Pietro Carlozzi

    (Institute of Ecosystem Study, National Research Council (CNR), Section of Florence, Polo Scientifico, Via Madonna del Piano n. 10, Sesto Fiorentino (FI) IT-50019, Italy)

Abstract

The aim of this study was to induce lipid accumulation in Chlorella cells by creating stressful growth conditions. Chlorella vulgaris CCALA 896 was grown under various batch growth modes in basal and modified BG-11 and Kolkwitz culture broths, using a continuous light regimen of 150 µE/m 2 /s, at 30 °C. In order to perform the experiments, two indoor photobioreactor shapes were used: a cylindrical glass photobioreactor (C G PBR) with a working volume of 350 mL, and a flat glass photobioreactor (F G PBR) with a working volume of 550 mL. Stress-eliciting conditions, such as nitrogen and phosphorous starvation, were imposed in order to induce lipid accumulation. The results demonstrated that more than 56% of the lipids can be accumulated in Chlorella biomass grown under two-phase batch growth conditions. The highest biomass productivity of 0.30 g/L/d was obtained at the highest nominal dilution rate (0.167 day −1 ) during a semi-continuous regimen, using a modified Kolkwitz medium. During the pH-stress cycles, the amount of lipids did not increase significantly and a flocculation of Chlorella cells was noted.

Suggested Citation

  • Sigita Vaičiulytė & Giulia Padovani & Jolanta Kostkevičienė & Pietro Carlozzi, 2014. "Batch Growth of Chlorella Vulgaris CCALA 896 versus Semi-Continuous Regimen for Enhancing Oil-Rich Biomass Productivity," Energies, MDPI, vol. 7(6), pages 1-18, June.
    • Handle: RePEc:gam:jeners:v:7:y:2014:i:6:p:3840-3857:d:37271
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    References listed on IDEAS

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    1. Mata, Teresa M. & Martins, António A. & Caetano, Nidia. S., 2010. "Microalgae for biodiesel production and other applications: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 14(1), pages 217-232, January.
    2. Demirbas, Ayhan, 2007. "Importance of biodiesel as transportation fuel," Energy Policy, Elsevier, vol. 35(9), pages 4661-4670, September.
    3. Kalpesh K. Sharma & Holger Schuhmann & Peer M. Schenk, 2012. "High Lipid Induction in Microalgae for Biodiesel Production," Energies, MDPI, vol. 5(5), pages 1-22, May.
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    2. Peter, Angela Paul & Koyande, Apurav Krishna & Chew, Kit Wayne & Ho, Shih-Hsin & Chen, Wei-Hsin & Chang, Jo-Shu & Krishnamoorthy, Rambabu & Banat, Fawzi & Show, Pau Loke, 2022. "Continuous cultivation of microalgae in photobioreactors as a source of renewable energy: Current status and future challenges," Renewable and Sustainable Energy Reviews, Elsevier, vol. 154(C).
    3. Okoro, Victor & Azimov, Ulugbek & Munoz, Jose & Hernandez, Hector H. & Phan, Anh N., 2019. "Microalgae cultivation and harvesting: Growth performance and use of flocculants - A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 115(C).
    4. Chen, Hui & Wang, Jie & Zheng, Yanli & Zhan, Jiao & He, Chenliu & Wang, Qiang, 2018. "Algal biofuel production coupled bioremediation of biomass power plant wastes based on Chlorella sp. C2 cultivation," Applied Energy, Elsevier, vol. 211(C), pages 296-305.

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