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Growth and Production of Lipids in Raphidocelis subcapitata Immobilized in Sodium Alginate Beads

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  • Amel Benasla

    (Station Expérimentale des Procédés Pilotes en Environnement, École de Technologie Supérieure, Université du Québec, 1100, rue Notre-Dame Ouest Local A-1500, Montréal, QC H3C 1K3, Canada)

  • Robert Hausler

    (Station Expérimentale des Procédés Pilotes en Environnement, École de Technologie Supérieure, Université du Québec, 1100, rue Notre-Dame Ouest Local A-1500, Montréal, QC H3C 1K3, Canada)

Abstract

The growth and production of lipids in the green microalga Raphidocelis subcapitata immobilized in alginate gel are studied. The beads are made from alginate (2% w / v ) and CaCl 2 (1% w / v ). The dry weight, the concentration of cells, and the lipid content are determined after dissolution of the beads in a sodium phosphate buffer. The results show that variations in biomass do not reflect variations in the number of cells in R. subcapitata . Cells divide more rapidly (G c = 3.45 ± 0.3 days) than biomass is produced (G m = 4.1 ± 0.4 days) during the exponential growth phase. Therefore, the average mass of the immobilized cells decreases until it reaches its minimum at the end of the exponential phase. Thus, during the stationary phase, cell division ceases while biomass production continues, and the average mass of the immobilized cells increases. In the present study, it is shown that this increase is due to the accumulation of lipids following the depletion of nitrates and phosphates in the culture medium. A lipid content of 24.7 ± 2.5% (dcw) and a lipid productivity of LP = 29.8 ± 3.0 mg/L/day are recorded at the end of culture. These results suggest that immobilized R. subcapitata has promising potential for biodiesel production.

Suggested Citation

  • Amel Benasla & Robert Hausler, 2020. "Growth and Production of Lipids in Raphidocelis subcapitata Immobilized in Sodium Alginate Beads," Energies, MDPI, vol. 13(2), pages 1-10, January.
    • Handle: RePEc:gam:jeners:v:13:y:2020:i:2:p:506-:d:311152
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    References listed on IDEAS

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    1. J. Pires & A. Gonçalves & F. Martins & M. Alvim-Ferraz & M. Simões, 2014. "Effect of light supply on CO 2 capture from atmosphere by Chlorella vulgaris and Pseudokirchneriella subcapitata," Mitigation and Adaptation Strategies for Global Change, Springer, vol. 19(7), pages 1109-1117, October.
    2. Khan, Shakeel A. & Rashmi & Hussain, Mir Z. & Prasad, S. & Banerjee, U.C., 2009. "Prospects of biodiesel production from microalgae in India," Renewable and Sustainable Energy Reviews, Elsevier, vol. 13(9), pages 2361-2372, December.
    3. Martin Olofsson & Teresa Lamela & Emmelie Nilsson & Jean Pascal Bergé & Victória Del Pino & Pauliina Uronen & Catherine Legrand, 2012. "Seasonal Variation of Lipids and Fatty Acids of the Microalgae Nannochloropsis oculata Grown in Outdoor Large-Scale Photobioreactors," Energies, MDPI, vol. 5(5), pages 1-16, May.
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