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Different Mutations Providing Target Site Resistance to ALS- and ACCase-Inhibiting Herbicides in Echinochloa spp. from Rice Fields

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  • Ignacio Amaro-Blanco

    (Plant Protection Department, Extremadura Scientific and Technological Research Center (CICYTEX), Ctra. AV, km 372, Guadajira, 06187 Badajoz, Spain
    This work is part of the PhD thesis of the first author.)

  • Yolanda Romano

    (Plant Protection Department, Extremadura Scientific and Technological Research Center (CICYTEX), Ctra. AV, km 372, Guadajira, 06187 Badajoz, Spain)

  • Jose Antonio Palmerin

    (Plant Health Service, Government of Extremadura, Ctra. Miajadas, km 2,5, Don Benito, 06400 Badajoz, Spain)

  • Raquel Gordo

    (Plant Protection Department, Extremadura Scientific and Technological Research Center (CICYTEX), Ctra. AV, km 372, Guadajira, 06187 Badajoz, Spain)

  • Candelario Palma-Bautista

    (Department of Agricultural Chemistry and Soil Science, University of Cordoba, Ctra. N-IV, km 396, 14071 Cordoba, Spain)

  • Rafael De Prado

    (Department of Agricultural Chemistry and Soil Science, University of Cordoba, Ctra. N-IV, km 396, 14071 Cordoba, Spain)

  • María Dolores Osuna

    (Plant Protection Department, Extremadura Scientific and Technological Research Center (CICYTEX), Ctra. AV, km 372, Guadajira, 06187 Badajoz, Spain)

Abstract

Echinochloa spp. is one of the most invasive weeds in rice fields worldwide. Acetolactate synthase (ALS) and acetyl-CoA carboxylase (ACCase) inhibiting herbicides are two of the most widely used rice herbicides. However, overuse has led to the resistance evolution of Echinochloa spp. to penoxsulam (ALS-inhibitor) and cyhalofop-methyl (ACCase-inhibitor). In this work, 137 different Echinochloa spp. populations were collected in different rice fields in Extremadura (western Spain) where lack of control was detected. Target-site based resistance (by sequencing ALS and ACCase gene) and characterization of Echinochloa species at the molecular level (based on PCR-RFLP analyses) were carried out in those populations. Most of the populations studied (111 of 137) belong to the E. oryzicola / E. oryzoides group. Three-point mutations were identified in ALS genes: Pro197Ser, Pro197Thr, and Ser653Asn, the first being the most frequent substitution in resistant plants. In the ACCase gene, the Ile1781Leu substitution was found. In both ALS and ACCase sequencing, evidence of heterozygosity was also observed. To assess whether cross-resistance patterns differed between mutations, two populations belonging to the E. oryzicola / E. oryzoides group had its most frequent mutations (Pro197Ser, population ech3-14 and Ile1781Leu, population ech114-10) chosen to be carried out in a dose-response assay. It was confirmed that Pro197Ser conferred resistance to triazolopyrimidine, imidazolinone, sulfonylurea, and pyrimidinyl benzoate families. On the other hand, the Ile1781Leu change gave resistance to aryloxyphenoxypropionate and cyclohexanedione families. Of the authorized herbicides in rice in Spain, more that 80% belong to these families. It is therefore important that farmers carry out an integrated control system that combines both chemical and non-chemical tools.

Suggested Citation

  • Ignacio Amaro-Blanco & Yolanda Romano & Jose Antonio Palmerin & Raquel Gordo & Candelario Palma-Bautista & Rafael De Prado & María Dolores Osuna, 2021. "Different Mutations Providing Target Site Resistance to ALS- and ACCase-Inhibiting Herbicides in Echinochloa spp. from Rice Fields," Agriculture, MDPI, vol. 11(5), pages 1-12, April.
    • Handle: RePEc:gam:jagris:v:11:y:2021:i:5:p:382-:d:541924
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    References listed on IDEAS

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    1. Christian Ritz & Florent Baty & Jens C Streibig & Daniel Gerhard, 2015. "Dose-Response Analysis Using R," PLOS ONE, Public Library of Science, vol. 10(12), pages 1-13, December.
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    Cited by:

    1. Luis Avila & Nilda Roma-Burgos, 2023. "Herbicide Physiology and Environmental Fate," Agriculture, MDPI, vol. 13(6), pages 1-2, May.

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