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Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol using immobilized cells of Klebsiella pneumoniae BLh-1

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  • Aquino de Souza, Elisangela
  • Rossi, Daniele Misturini
  • Záchia Ayub, Marco Antônio

Abstract

The bioconversion of residual glycerol obtained from biodiesel synthesis into 1,3-propanediol (1,3-PD) was carried out using Ca-alginate immobilized-cells cultures of Klebsiella pneumoniae BLh-1. The immobilization parameters bead size and cell concentration were determined for the best possible productivity and were found to be 3.4 mm and 100 mg mL−1, respectively. In 12 h-batch cultivations, immobilized cultures showed a 1,3-PD productivity of 1.85 g L−1 h−1, up from 1.22 g L−1 h−1 obtained in 16 h-batch cultivations using free-suspended cells. The possibility of reuse of the biocatalysts was tested in 5-cycles repeated batch cultures, with entrapped cells keeping their operational stability and biological activity, with 1,3-PD productivities as high as 4.48 g L−1 h−1. Other important metabolites such as 2,3-butanediol and ethanol were also produced (10.11 g L−1 and 3.71 g L−1, respectively) during the repeated batches. These results show the feasibility of using and further developing immobilized-cell systems of K. pneumoniae for the efficient bioprocessing of residual glycerol.

Suggested Citation

  • Aquino de Souza, Elisangela & Rossi, Daniele Misturini & Záchia Ayub, Marco Antônio, 2014. "Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol using immobilized cells of Klebsiella pneumoniae BLh-1," Renewable Energy, Elsevier, vol. 72(C), pages 253-257.
  • Handle: RePEc:eee:renene:v:72:y:2014:i:c:p:253-257
    DOI: 10.1016/j.renene.2014.07.030
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    References listed on IDEAS

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    1. Rossi, Daniele Misturini & da Costa, Janaína Berne & de Souza, Elisangela Aquino & Peralba, Maria do Carmo Ruaro & Ayub, Marco Antônio Záchia, 2012. "Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia," Renewable Energy, Elsevier, vol. 39(1), pages 223-227.
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    Cited by:

    1. Vivek, Narisetty & Christopher, Meera & Kumar, M. Kiran & Castro, Eulogio & Binod, Parameswaran & Pandey, Ashok, 2018. "Pentose rich acid pretreated liquor as co-substrate for 1,3-propanediol production," Renewable Energy, Elsevier, vol. 129(PB), pages 794-799.
    2. Tawakalt Ayodele & Kudirat Alarape & Ibrahim Adebayo Bello & Abodunrin Tijani & Liadi Musiliu & Ademola Hammed, 2024. "Microbial Protein Production Using Lignocellulosic Biomass (Switchgrass) and Klebsiella oxytoca M5A1 —A Nitrogen Fixer," Sustainability, MDPI, vol. 16(13), pages 1-14, June.

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