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Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae

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  • Rossi, Daniele Misturini
  • de Souza, Elisangela Aquino
  • Flôres, Simone Hickmann
  • Ayub, Marco Antônio Záchia

Abstract

A stepwise strategy was devised to optimize the bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol (1,3-PD) by a new strain Klebsiella pneumoniae. This strain is unique in its ability to convert glycerol simultaneously into 1,3-PD. The influence of glycerol concentration, pH, temperature, nitrogen and mineral sources were first investigated using the Plackett–Burman (P–B) statistical design to screen the variables that significantly affect the production of 1,3-PD. Seven variables were statistically significant at 95% significance and a 27-3 fractional factorial design (FFD) was applied to further refine the optimization of culture conditions. Results showed that the ideal medium composition and culture conditions for the syntheses of 1,3-PD are: glycerol 65 g/L, yeast extract 5 g/L, peptone 5 g/L, (NH4)2SO4 7 g/L, K2HPO4 7 g/L, and temperature of 37 °C. Experiments in batch bioreactors under controlled pH produced up to 23.80 g/L of 1,3-PD and 12.30 g/L of ethanol, while in fed-batch cultivations a three-fold increase of 1,3-PD production (36.86 g/L) was obtained compared with the results of the FFD design.

Suggested Citation

  • Rossi, Daniele Misturini & de Souza, Elisangela Aquino & Flôres, Simone Hickmann & Ayub, Marco Antônio Záchia, 2013. "Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae," Renewable Energy, Elsevier, vol. 55(C), pages 404-409.
    • Handle: RePEc:eee:renene:v:55:y:2013:i:c:p:404-409
    DOI: 10.1016/j.renene.2012.12.048
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    1. Rossi, Daniele Misturini & da Costa, Janaína Berne & de Souza, Elisangela Aquino & Peralba, Maria do Carmo Ruaro & Ayub, Marco Antônio Záchia, 2012. "Bioconversion of residual glycerol from biodiesel synthesis into 1,3-propanediol and ethanol by isolated bacteria from environmental consortia," Renewable Energy, Elsevier, vol. 39(1), pages 223-227.
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    Cited by:

    1. Rahman, Md. Shafiqur & Xu, Chunbao (Charles) & Ma, Kesen & Guo, Haipeng & Qin, Wensheng, 2017. "Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3," Renewable Energy, Elsevier, vol. 114(PB), pages 1272-1280.
    2. Laura, Mitrea & Monica, Trif & Dan-Cristian, Vodnar, 2020. "The effect of crude glycerol impurities on 1,3-propanediol biosynthesis by Klebsiella pneumoniae DSMZ 2026," Renewable Energy, Elsevier, vol. 153(C), pages 1418-1427.

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