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Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3

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  • Rahman, Md. Shafiqur
  • Xu, Chunbao (Charles)
  • Ma, Kesen
  • Guo, Haipeng
  • Qin, Wensheng

Abstract

The booming of biodiesel industry all over the world has led to generate a large amount (10% v/v) of crude glycerol, created an oversupply problem. Herein, we compared the product concentrations of major metabolic products attained from pure and crude glycerol biotransformation process using an adapted mutant strain Klebsiella variicola SW3. Real-time qPCR and glycerol dehydrogenase (GDH) enzyme activity assay revealed that the overexpression of GDH gene resulted in an increased GDH enzyme activity, led to a markedly boosted 2,3-butanediol (2,3-BD) production. Based on these results, the SW3 strain obtained from wild type strain Klebsiella variicola SRP3 displayed a 1.39-fold increased 2,3-BD production of 82.5 g/L from 59.3 g/L, yielding 0.62 g/g using pure glycerol. However, in a batch culture, a final 33.5 g/L of 2,3-BD was accumulated within 96 h from 50 g/L glycerol. Moreover, the strain SW3 withstanding high concentration (200 g/L) of crude glycerol displayed 64.9 and 29.25 g/L 2,3-BD in fed-batch and batch cultures respectively. Therefore, this bioconversion of crude glycerol to 2,3-BD –a value-added green product with potential industrial applications as a liquid fuel or fuel additive would represent a remarkable alternative to add value to the biodiesel production helping biodiesel industries development.

Suggested Citation

  • Rahman, Md. Shafiqur & Xu, Chunbao (Charles) & Ma, Kesen & Guo, Haipeng & Qin, Wensheng, 2017. "Utilization of by-product glycerol from bio-diesel plants as feedstock for 2,3-butanediol accumulation and biosynthesis genes response of Klebsiella variicola SW3," Renewable Energy, Elsevier, vol. 114(PB), pages 1272-1280.
  • Handle: RePEc:eee:renene:v:114:y:2017:i:pb:p:1272-1280
    DOI: 10.1016/j.renene.2017.07.080
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    References listed on IDEAS

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    1. Rossi, Daniele Misturini & de Souza, Elisangela Aquino & Flôres, Simone Hickmann & Ayub, Marco Antônio Záchia, 2013. "Conversion of residual glycerol from biodiesel synthesis into 1,3-propanediol by a new strain of Klebsiella pneumoniae," Renewable Energy, Elsevier, vol. 55(C), pages 404-409.
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    Cited by:

    1. Laura, Mitrea & Monica, Trif & Dan-Cristian, Vodnar, 2020. "The effect of crude glycerol impurities on 1,3-propanediol biosynthesis by Klebsiella pneumoniae DSMZ 2026," Renewable Energy, Elsevier, vol. 153(C), pages 1418-1427.
    2. Rodrigues, Caroline Varella & Rios Alcaraz, Francisco Abraham & Nespeca, Maurílio Gustavo & Rodrigues, Aline Varella & Motteran, Fabrício & Tallarico Adorno, Maria Angela & Varesche, Maria Bernadete A, 2020. "Biohydrogen production in an integrated biosystem using crude glycerol from waste cooking oils," Renewable Energy, Elsevier, vol. 162(C), pages 701-711.
    3. Vávra, Aleš & Hájek, Martin & Kocián, David, 2021. "The influence of vegetable oils composition on separation of transesterification products, especially quality of glycerol," Renewable Energy, Elsevier, vol. 176(C), pages 262-268.

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    1. Laura, Mitrea & Monica, Trif & Dan-Cristian, Vodnar, 2020. "The effect of crude glycerol impurities on 1,3-propanediol biosynthesis by Klebsiella pneumoniae DSMZ 2026," Renewable Energy, Elsevier, vol. 153(C), pages 1418-1427.

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