IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v230y2024ics0960148124008619.html
   My bibliography  Save this article

Production of high levels of 2,3-butanediol from renewable crude glycerol by Klebsiella pneumoniae GEM167 using metabolic engineering and control of oxygen transfer efficiency

Author

Listed:
  • Jo, Min-Ho
  • Ju, Jung-Hyun
  • Heo, Sun-Yeon
  • Son, Chang-Bum
  • Jeong, Ki Jun
  • Oh, Baek-Rock

Abstract

Numerous industries use 2,3-butanediol as a bulk chemical. This study aimed to develop a technique for the commercial production of 2,3-butanediol from glycerol using K. pneumoniae GEM167 strain with enhanced oxidative pathways. We developed K. pneumoniae GEM167ΔadhEΔldhA, a strain that has decreased production of competitive metabolites (ethanol and lactate) and increased production of 2,3-butanediol. We investigated the correlation between the oxygen transfer coefficient and 2,3-butanediol production by modifying the agitation speed and aeration rate. K. pneumoniae GEM167ΔadhEΔldhA provided a high titer (102.9 ± 3.1 g/L) and productivity (2.14 ± 0.06 g/L/h) of 2,3-butanediol with no detectable 1,3-propaendiol production under specific culture conditions (37 °C, 700 rpm, 2.0 vvm, and pH 6.0). To the best of our knowledge, the productivity of 2,3-butanediol from glycerol in this study is the highest yet reported. And the substitution with crude glycerol enhanced the titer (105.3 ± 2.8 g/L) and productivity (2.19 ± 0.06 g/L/h) of 2,3-butanediol.

Suggested Citation

  • Jo, Min-Ho & Ju, Jung-Hyun & Heo, Sun-Yeon & Son, Chang-Bum & Jeong, Ki Jun & Oh, Baek-Rock, 2024. "Production of high levels of 2,3-butanediol from renewable crude glycerol by Klebsiella pneumoniae GEM167 using metabolic engineering and control of oxygen transfer efficiency," Renewable Energy, Elsevier, vol. 230(C).
    • Handle: RePEc:eee:renene:v:230:y:2024:i:c:s0960148124008619
    DOI: 10.1016/j.renene.2024.120793
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148124008619
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2024.120793?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:230:y:2024:i:c:s0960148124008619. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    We have no bibliographic references for this item. You can help adding them by using this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.