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Production of high levels of 2,3-butanediol from renewable crude glycerol by Klebsiella pneumoniae GEM167 using metabolic engineering and control of oxygen transfer efficiency

Author

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  • Jo, Min-Ho
  • Ju, Jung-Hyun
  • Heo, Sun-Yeon
  • Son, Chang-Bum
  • Jeong, Ki Jun
  • Oh, Baek-Rock

Abstract

Numerous industries use 2,3-butanediol as a bulk chemical. This study aimed to develop a technique for the commercial production of 2,3-butanediol from glycerol using K. pneumoniae GEM167 strain with enhanced oxidative pathways. We developed K. pneumoniae GEM167ΔadhEΔldhA, a strain that has decreased production of competitive metabolites (ethanol and lactate) and increased production of 2,3-butanediol. We investigated the correlation between the oxygen transfer coefficient and 2,3-butanediol production by modifying the agitation speed and aeration rate. K. pneumoniae GEM167ΔadhEΔldhA provided a high titer (102.9 ± 3.1 g/L) and productivity (2.14 ± 0.06 g/L/h) of 2,3-butanediol with no detectable 1,3-propaendiol production under specific culture conditions (37 °C, 700 rpm, 2.0 vvm, and pH 6.0). To the best of our knowledge, the productivity of 2,3-butanediol from glycerol in this study is the highest yet reported. And the substitution with crude glycerol enhanced the titer (105.3 ± 2.8 g/L) and productivity (2.19 ± 0.06 g/L/h) of 2,3-butanediol.

Suggested Citation

  • Jo, Min-Ho & Ju, Jung-Hyun & Heo, Sun-Yeon & Son, Chang-Bum & Jeong, Ki Jun & Oh, Baek-Rock, 2024. "Production of high levels of 2,3-butanediol from renewable crude glycerol by Klebsiella pneumoniae GEM167 using metabolic engineering and control of oxygen transfer efficiency," Renewable Energy, Elsevier, vol. 230(C).
    • Handle: RePEc:eee:renene:v:230:y:2024:i:c:s0960148124008619
    DOI: 10.1016/j.renene.2024.120793
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    References listed on IDEAS

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    1. Cheng Li & Keaton L. Lesnik & Hong Liu, 2013. "Microbial Conversion of Waste Glycerol from Biodiesel Production into Value-Added Products," Energies, MDPI, vol. 6(9), pages 1-30, September.
    2. Murugesan, A. & Umarani, C. & Subramanian, R. & Nedunchezhian, N., 2009. "Bio-diesel as an alternative fuel for diesel engines--A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 13(3), pages 653-662, April.
    3. Tayari, Sara & Abedi, Reza & Rahi, Abbas, 2020. "Comparative assessment of engine performance and emissions fueled with three different biodiesel generations," Renewable Energy, Elsevier, vol. 147(P1), pages 1058-1069.
    4. Jo, Min-Ho & Heo, Sun-Yeon & Ju, Jung-Hyun & Jeong, Ki Jun & Oh, Baek-Rock, 2024. "Efficient production of isobutanol from glycerol in Klebsiella pneumoniae: Regulation of acetohydroxyacid synthase, a rate-limiting enzyme in isobutanol biosynthesis," Renewable Energy, Elsevier, vol. 223(C).
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