IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v201y2022ip1p486-498.html
   My bibliography  Save this article

RNA-seq based transcriptomic analysis of the non-conventional yeast Spathaspora passalidarum during Melle-boinot cell recycle in xylose-glucose mixtures

Author

Listed:
  • Neitzel, Thiago
  • Lima, Cleilton Santos
  • Hafemann, Eduardo
  • Paixão, Douglas Antonio Alvaredo
  • Junior, Joaquim Martins
  • Persinoti, Gabriela Felix
  • dos Santos, Leandro Vieira
  • Ienczak, Jaciane Lutz

Abstract

The Melle-Boinot is a promising process for second-generation ethanol production by xylose-fermenting yeasts. However, the impact of this process on the physiology of the non-conventional yeast Spathaspora passalidarum during second-generation ethanol production remains unclear. Therefore, we performed a transcriptomic analysis of S. passalidarum to determine the differences and global responses of differentially expressed genes (DEGs) during five fed-batch fermentations with cell recycle. A cycle-to-cycle metabolic reprogramming was observed resulting in an increase in ethanol yield (32%), volumetric productivity (33%), and titer (33%); and an expressive decrease of 94% of xylitol production. A broad set of pathways operated synergically such as fatty acid metabolism, N-glycan biosynthesis, glyoxylate and dicarboxylate metabolism, oxidative phosphorylation, glutathione metabolism and sulfur metabolism, indicating those as important mechanisms for cell recycling adaptations due to increased ethanol concentration, long-term ethanol exposure and osmotic stress. Together these results suggest that cellular energy was redirected towards the production of cell wall components enabling S. passalidarum cells to thrive on consecutive recycles. Furthermore, these results are instrumental to guide genetic-engineering efforts of xylose-fermenting yeasts to improve the productiveness and feasibility of renewable energy production from lignocellulosic biomass and hemicellulosic hydrolysates.

Suggested Citation

  • Neitzel, Thiago & Lima, Cleilton Santos & Hafemann, Eduardo & Paixão, Douglas Antonio Alvaredo & Junior, Joaquim Martins & Persinoti, Gabriela Felix & dos Santos, Leandro Vieira & Ienczak, Jaciane Lut, 2022. "RNA-seq based transcriptomic analysis of the non-conventional yeast Spathaspora passalidarum during Melle-boinot cell recycle in xylose-glucose mixtures," Renewable Energy, Elsevier, vol. 201(P1), pages 486-498.
    • Handle: RePEc:eee:renene:v:201:y:2022:i:p1:p:486-498
    DOI: 10.1016/j.renene.2022.10.108
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S096014812201610X
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2022.10.108?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Neitzel, Thiago & Lima, Cleilton Santos & Biazi, Luiz Eduardo & Collograi, Karen Cristina & Carvalho da Costa, Aline & Vieira dos Santos, Leandro & Ienczak, Jaciane Lutz, 2020. "Impact of the Melle-Boinot process on the enhancement of second-generation ethanol production by Spathaspora passalidarum," Renewable Energy, Elsevier, vol. 160(C), pages 1206-1216.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Ashraf Elfasakhany, 2021. "State of Art of Using Biofuels in Spark Ignition Engines," Energies, MDPI, vol. 14(3), pages 1-26, February.
    2. Martínez-Jimenez, F.D. & Pereira, I.O. & Ribeiro, M.P.A. & Sargo, C.R. & dos Santos, A.A. & Zanella, E. & Stambuk, B.U. & Ienczak, J.L. & Morais, E.R. & Costa, A.C., 2022. "Integration of first- and second-generation ethanol production: Evaluation of a mathematical model to describe sucrose and xylose co-fermentation by recombinant Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 192(C), pages 326-339.
    3. Mariana S. T. Amândio & Jorge M. S. Rocha & Luísa S. Serafim & Ana M. R. B. Xavier, 2021. "Cellulosic Bioethanol from Industrial Eucalyptus globulus Bark Residues Using Kraft Pulping as a Pretreatment," Energies, MDPI, vol. 14(8), pages 1-18, April.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:201:y:2022:i:p1:p:486-498. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.