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Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies

Author

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  • Li, Wen-Chao
  • Zhu, Jia-Qing
  • Zhao, Xiong
  • Qin, Lei
  • Xu, Tao
  • Zhou, Xiao
  • Li, Xia
  • Li, Bing-Zhi
  • Yuan, Ying-Jin

Abstract

Xylose utilization of engineered yeast is vulnerable to inhibitors generated during pretreatment of lignocellulose. In this study, adaptive evolution was applied to enhance the tolerance of xylose-fermenting strain. Compared to the parental strain, the ethanol yield was increased by 60% and 80% for the adapted strain (E7-403) when xylose was used as the sole carbon resource with 20% and 50% inhibitor cocktails, respectively. E7-403 removed furfural more effectively than parental strain (E7) in the fermentation with 100% inhibitor cocktails. In the fermentation with mixed sugar and high inhibitor concentration, glucose was depleted within 36 h for E7-403 while 6.1 g/L glucose was still left after 120 h for E7. Consequently, ethanol yield of E7-403 was 22.9% higher than that of E7. It was demonstrated that E7-403 strain exhibited an enhanced ability for regulating cellular reactive oxygen species, which alleviated the harmful effects of inhibitors. Meanwhile, E7-403 strain was further applied in co-culture and pre-fermentation process to improve xylose utilization.

Suggested Citation

  • Li, Wen-Chao & Zhu, Jia-Qing & Zhao, Xiong & Qin, Lei & Xu, Tao & Zhou, Xiao & Li, Xia & Li, Bing-Zhi & Yuan, Ying-Jin, 2019. "Improving co-fermentation of glucose and xylose by adaptive evolution of engineering xylose-fermenting Saccharomyces cerevisiae and different fermentation strategies," Renewable Energy, Elsevier, vol. 139(C), pages 1176-1183.
    • Handle: RePEc:eee:renene:v:139:y:2019:i:c:p:1176-1183
    DOI: 10.1016/j.renene.2019.03.028
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    References listed on IDEAS

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    1. Park, Yong Cheol & Kim, Jun Seok, 2012. "Comparison of various alkaline pretreatment methods of lignocellulosic biomass," Energy, Elsevier, vol. 47(1), pages 31-35.
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    2. Rosen, Yan & Mamane, Hadas & Gerchman, Yoram, 2021. "Immersed ozonation of agro-wastes as an effective pretreatment method in bioethanol production," Renewable Energy, Elsevier, vol. 174(C), pages 382-390.
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    4. Therasme, Obste & Volk, Timothy A. & Fortier, Marie-Odile & Kim, Youngwoon & Wood, Christopher D. & Ha, HakSoo & Ali, Atif & Brown, Tristan & Malmsheimer, Robert, 2022. "Carbon footprint of biofuels production from forest biomass using hot water extraction and biochemical conversion in the Northeast United States," Energy, Elsevier, vol. 241(C).
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    6. Qu, Chunyun & Dai, Kaiqun & Liu, Gongliang & Wang, Jufang, 2023. "Engineering Thermoanaerobacterium aotearoense SCUT27 with the deficiency of a hypothetic protein regulated by ArgR1864 for enhanced ethanol production from lignocellulosic hydrolysates," Renewable Energy, Elsevier, vol. 216(C).
    7. Tran, Phuong Hoang Nguyen & Jung, Je Hyeong & Ko, Ja Kyong & Gong, Gyeongtaek & Um, Youngsoon & Lee, Sun-Mi, 2023. "Co-production of ethanol and polyhydroxybutyrate from lignocellulosic biomass using an engineered Saccharomyces cerevisiae," Renewable Energy, Elsevier, vol. 212(C), pages 601-611.
    8. Shen, Guannan & Yuan, Xinchuan & Chen, Sitong & Liu, Shuangmei & Jin, Mingjie, 2022. "High titer cellulosic ethanol production from sugarcane bagasse via DLCA pretreatment and process development without washing/detoxifying pretreated biomass," Renewable Energy, Elsevier, vol. 186(C), pages 904-913.
    9. Li, Jun & Zhao, Renyong & Xu, Youjie & Wu, Xiaorong & Bean, Scott R. & Wang, Donghai, 2022. "Fuel ethanol production from starchy grain and other crops: An overview on feedstocks, affecting factors, and technical advances," Renewable Energy, Elsevier, vol. 188(C), pages 223-239.

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