IDEAS home Printed from https://ideas.repec.org/a/eee/renene/v135y2019icp936-944.html
   My bibliography  Save this article

Innovative screening approach for the identification of triacylglycerol accumulating oleaginous strains

Author

Listed:
  • Patel, Alok
  • Pruthi, Vikas
  • Pruthi, Parul A.

Abstract

Currently, triacylglycerides (TAG) accumulation in the form of lipid droplets (LDs) in oleaginous microorganisms is of immense importance due to their ability to get transesterified into value-added products in the form of biodiesel. Hence, in order to search for oleaginous microorganisms having high lipid content among a wide range of samples from different niches, there is a compulsive need to develop simple, reliable and rapid methods for screening of TAG accumulating strains. Conventional methods require multistep processes for the isolation, cultivation, extraction and estimation of lipids to identify oleagenic strains. To overcome these challenges, we are proposing an easy, live cell imaging technique for the estimation of lipids via visualization of TAG accumulation in probable strains at the single cell level that gives real-time monitoring of intracellular lipid accumulation in yeasts. In this screening technique, only 100 μl of specific neutral lipid accumulating medium was used to grow the isolated culture in the microtiter plate. The harvested cells were stained with LipidTOX™ Green and visualized by a LED based digital inverted fluorescence microscope. Among 446 yeast colonies screened, maximum lipid producing yeast strains Rhodosporidium kratochvilovae HIMPA1 and Rhodotorula minuta, having supersized lipid body of 5.05 ± 0.87 μm and 4.46 ± 0.61 μm, respectively, were identified as potential candidates for biodiesel production. To the best of our knowledge, this is the first report of using LipidTOX™ Green for the staining of lipid droplets present in yeast cells as per the literature.

Suggested Citation

  • Patel, Alok & Pruthi, Vikas & Pruthi, Parul A., 2019. "Innovative screening approach for the identification of triacylglycerol accumulating oleaginous strains," Renewable Energy, Elsevier, vol. 135(C), pages 936-944.
    • Handle: RePEc:eee:renene:v:135:y:2019:i:c:p:936-944
    DOI: 10.1016/j.renene.2018.12.078
    as

    Download full text from publisher

    File URL: http://www.sciencedirect.com/science/article/pii/S0960148118315258
    Download Restriction: Full text for ScienceDirect subscribers only
    File URL: https://libkey.io/10.1016/j.renene.2018.12.078?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Zhang, Zhi-Hui & Balasubramanian, Rajasekhar, 2016. "Investigation of particulate emission characteristics of a diesel engine fueled with higher alcohols/biodiesel blends," Applied Energy, Elsevier, vol. 163(C), pages 71-80.
    2. Patel, Alok & Arora, Neha & Sartaj, Km & Pruthi, Vikas & Pruthi, Parul A., 2016. "Sustainable biodiesel production from oleaginous yeasts utilizing hydrolysates of various non-edible lignocellulosic biomasses," Renewable and Sustainable Energy Reviews, Elsevier, vol. 62(C), pages 836-855.
    3. Meng, Xin & Yang, Jianming & Xu, Xin & Zhang, Lei & Nie, Qingjuan & Xian, Mo, 2009. "Biodiesel production from oleaginous microorganisms," Renewable Energy, Elsevier, vol. 34(1), pages 1-5.
    4. Patel, Alok & Arora, Neha & Mehtani, Juhi & Pruthi, Vikas & Pruthi, Parul A., 2017. "Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 77(C), pages 604-616.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. Mauricio Ramirez-Castrillon & Victoria P. Jaramillo-Garcia & Helio Lopes Barros & João A. Pêgas Henriques & Valter Stefani & Patricia Valente, 2020. "Dataset of Nile Red Fluorescence Readings with Different Yeast Strains, Solvents, and Incubation Times," Data, MDPI, vol. 5(3), pages 1-6, September.
    2. Alok Patel & Liwen Mu & Yijun Shi & Ulrika Rova & Paul Christakopoulos & Leonidas Matsakas, 2021. "Single-Cell Oils from Oleaginous Microorganisms as Green Bio-Lubricants: Studies on Their Tribological Performance," Energies, MDPI, vol. 14(20), pages 1-17, October.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Siwina, Siraprapha & Leesing, Ratanaporn, 2021. "Bioconversion of durian (Durio zibethinus Murr.) peel hydrolysate into biodiesel by newly isolated oleaginous yeast Rhodotorula mucilaginosa KKUSY14," Renewable Energy, Elsevier, vol. 163(C), pages 237-245.
    2. Patel, Alok & Arora, Neha & Mehtani, Juhi & Pruthi, Vikas & Pruthi, Parul A., 2017. "Assessment of fuel properties on the basis of fatty acid profiles of oleaginous yeast for potential biodiesel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 77(C), pages 604-616.
    3. Caporusso, Antonio & De Bari, Isabella & Liuzzi, Federico & Albergo, Roberto & Valerio, Vito & Viola, Egidio & Pietrafesa, Rocchina & Siesto, Gabriella & Capece, Angela, 2023. "Optimized conversion of wheat straw into single cell oils by Yarrowia lipolytica and Lipomyces tetrasporus and synthesis of advanced biofuels," Renewable Energy, Elsevier, vol. 202(C), pages 184-195.
    4. Leesing, Ratanaporn & Siwina, Siraprapha & Ngernyen, Yuvarat & Fiala, Khanittha, 2022. "Innovative approach for co-production of single cell oil (SCO), novel carbon-based solid acid catalyst and SCO-based biodiesel from fallen Dipterocarpus alatus leaves," Renewable Energy, Elsevier, vol. 185(C), pages 47-60.
    5. Zhao, Man & Wang, Yanan & Zhou, Wenting & Zhou, Wei & Gong, Zhiwei, 2023. "Co-valorization of crude glycerol and low-cost substrates via oleaginous yeasts to micro-biodiesel: Status and outlook," Renewable and Sustainable Energy Reviews, Elsevier, vol. 180(C).
    6. Leesing, Ratanaporn & Somdee, Theerasak & Siwina, Siraprapha & Ngernyen, Yuvarat & Fiala, Khanittha, 2022. "Production of 2G and 3G biodiesel, yeast oil, and sulfonated carbon catalyst from waste coconut meal: An integrated cascade biorefinery approach," Renewable Energy, Elsevier, vol. 199(C), pages 1093-1104.
    7. Chuengcharoenphanich, Nuttha & Watsuntorn, Wannapawn & Qi, Wei & Wang, Zhongming & Hu, Yunzi & Chulalaksananukul, Warawut, 2023. "The potential of biodiesel production from grasses in Thailand through consolidated bioprocessing using a cellulolytic oleaginous yeast, Cyberlindnera rhodanensis CU-CV7," Energy, Elsevier, vol. 263(PB).
    8. Ko, Ja Kyong & Lee, Jae Hoon & Jung, Je Hyeong & Lee, Sun-Mi, 2020. "Recent advances and future directions in plant and yeast engineering to improve lignocellulosic biofuel production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 134(C).
    9. Rishibha Dixit & Surendra Singh & Manoj Kumar Enamala & Alok Patel, 2022. "Effect of Various Growth Medium on the Physiology and De Novo Lipogenesis of a Freshwater Microalga Scenedesmus rotundus -MG910488 under Autotrophic Condition," Clean Technol., MDPI, vol. 4(3), pages 1-19, August.
    10. Zhang, Xiaolei & Yan, Song & Tyagi, Rajeshwar D. & Surampalli, RaoY. & Valéro, Jose R., 2014. "Wastewater sludge as raw material for microbial oils production," Applied Energy, Elsevier, vol. 135(C), pages 192-201.
    11. Sánchez-Bayo, Alejandra & López-Chicharro, Daniel & Morales, Victoria & Espada, Juan José & Puyol, Daniel & Martínez, Fernando & Astals, Sergi & Vicente, Gemma & Bautista, Luis Fernando & Rodríguez, R, 2020. "Biodiesel and biogas production from Isochrysis galbana using dry and wet lipid extraction: A biorefinery approach," Renewable Energy, Elsevier, vol. 146(C), pages 188-195.
    12. Qiang Li & Rasool Kamal & Qian Wang & Xue Yu & Zongbao Kent Zhao, 2020. "Lipid Production from Amino Acid Wastes by the Oleaginous Yeast Rhodosporidium toruloides," Energies, MDPI, vol. 13(7), pages 1-9, April.
    13. Dar, Rouf Ahmad & Tsui, To-Hung & Zhang, Le & Tong, Yen Wah & Sharon, Sigal & Shoseyov, Oded & Liu, Ronghou, 2024. "Fermentation of organic wastes through oleaginous microorganisms for lipid production - Challenges and opportunities," Renewable and Sustainable Energy Reviews, Elsevier, vol. 195(C).
    14. Zhang, X.L. & Yan, S. & Tyagi, R.D. & Surampalli, R.Y., 2013. "Biodiesel production from heterotrophic microalgae through transesterification and nanotechnology application in the production," Renewable and Sustainable Energy Reviews, Elsevier, vol. 26(C), pages 216-223.
    15. Suiuay, Chokchai & Laloon, Kittipong & Katekaew, Somporn & Senawong, Kritsadang & Noisuwan, Phakamat & Sudajan, Somposh, 2020. "Effect of gasoline-like fuel obtained from hard-resin of Yang (Dipterocarpus alatus) on single cylinder gasoline engine performance and exhaust emissions," Renewable Energy, Elsevier, vol. 153(C), pages 634-645.
    16. Marco Castellini & Stefano Ubertini & Diego Barletta & Ilaria Baffo & Pietro Buzzini & Marco Barbanera, 2021. "Techno-Economic Analysis of Biodiesel Production from Microbial Oil Using Cardoon Stalks as Carbon Source," Energies, MDPI, vol. 14(5), pages 1-21, March.
    17. Ullah, Zahoor & Bustam, Mohamad Azmi & Man, Zakaria, 2015. "Biodiesel production from waste cooking oil by acidic ionic liquid as a catalyst," Renewable Energy, Elsevier, vol. 77(C), pages 521-526.
    18. EL-Seesy, Ahmed I. & Hassan, Hamdy, 2019. "Investigation of the effect of adding graphene oxide, graphene nanoplatelet, and multiwalled carbon nanotube additives with n-butanol-Jatropha methyl ester on a diesel engine performance," Renewable Energy, Elsevier, vol. 132(C), pages 558-574.
    19. Nouri, Hoda & Moghimi, Hamid & Nikbakht Rad, Mahzad & Ostovar, Marjan & Farazandeh Mehr, Shima Sadat & Ghanaatian, Fateme & Talebi, Ahmad Farhad, 2019. "Enhanced growth and lipid production in oleaginous fungus, Sarocladium kiliense ADH17: Study on fatty acid profiling and prediction of biodiesel properties," Renewable Energy, Elsevier, vol. 135(C), pages 10-20.
    20. Guldhe, Abhishek & Singh, Poonam & Kumari, Sheena & Rawat, Ismail & Permaul, Kugen & Bux, Faizal, 2016. "Biodiesel synthesis from microalgae using immobilized Aspergillus niger whole cell lipase biocatalyst," Renewable Energy, Elsevier, vol. 85(C), pages 1002-1010.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:eee:renene:v:135:y:2019:i:c:p:936-944. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Catherine Liu (email available below). General contact details of provider: http://www.journals.elsevier.com/renewable-energy .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.