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Nutrient limitation as a strategy for increasing starch accumulation in microalgae

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  • Dragone, Giuliano
  • Fernandes, Bruno D.
  • Abreu, Ana P.
  • Vicente, António A.
  • Teixeira, José A.

Abstract

Increasing microalgal starch content by nutrient limitation has been regarded as an affordable approach for the production of third generation bioethanol. This work evaluated starch accumulation in Chlorella vulgaris P12 under different initial concentrations of nitrogen (0–2.2gureaL−1) and iron (0–0.08gFeNa-EDTAL−1) sources, using a central composite design (CCD) for two factors. The obtained model: Starch content (%)=8.220−16.133X1+13.850X12, relating starch accumulation in microalgae with the coded level for initial urea concentration in the growth medium (X1) presented a good concordance between the predicted and experimental values (R2=0.94). Since accumulation of starch occurred at nitrogen depletion conditions under which the cell growth was much slower than that observed during nitrogen supplemented cultivations, a two-stage cultivation process for high starch accumulation (>40%) and cell growth of C. vulgaris was proposed: a first cultivation stage using nitrogen- and iron-supplemented medium (initial urea and FeNa-EDTA concentrations of 1.1 and 0.08gL−1, respectively), followed by a second cultivation stage in a nitrogen- and iron-free medium. The high starch content obtained suggests C. vulgaris P12 as a very promising feedstock for bioethanol production.

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  • Dragone, Giuliano & Fernandes, Bruno D. & Abreu, Ana P. & Vicente, António A. & Teixeira, José A., 2011. "Nutrient limitation as a strategy for increasing starch accumulation in microalgae," Applied Energy, Elsevier, vol. 88(10), pages 3331-3335.
    • Handle: RePEc:eee:appene:v:88:y:2011:i:10:p:3331-3335
    DOI: 10.1016/j.apenergy.2011.03.012
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    1. Hirano, Atsushi & Ueda, Ryohei & Hirayama, Shin & Ogushi, Yasuyuki, 1997. "CO2 fixation and ethanol production with microalgal photosynthesis and intracellular anaerobic fermentation," Energy, Elsevier, vol. 22(2), pages 137-142.
    2. Amaro, Helena M. & Guedes, A. Catarina & Malcata, F. Xavier, 2011. "Advances and perspectives in using microalgae to produce biodiesel," Applied Energy, Elsevier, vol. 88(10), pages 3402-3410.
    3. Huang, GuanHua & Chen, Feng & Wei, Dong & Zhang, XueWu & Chen, Gu, 2010. "Biodiesel production by microalgal biotechnology," Applied Energy, Elsevier, vol. 87(1), pages 38-46, January.
    4. William G. Sunda & Susan A. Huntsman, 1997. "Interrelated influence of iron, light and cell size on marine phytoplankton growth," Nature, Nature, vol. 390(6658), pages 389-392, November.
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    2. Safi, Carl & Zebib, Bachar & Merah, Othmane & Pontalier, Pierre-Yves & Vaca-Garcia, Carlos, 2014. "Morphology, composition, production, processing and applications of Chlorella vulgaris: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 35(C), pages 265-278.
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    11. Cheah, Wai Yan & Ling, Tau Chuan & Show, Pau Loke & Juan, Joon Ching & Chang, Jo-Shu & Lee, Duu-Jong, 2016. "Cultivation in wastewaters for energy: A microalgae platform," Applied Energy, Elsevier, vol. 179(C), pages 609-625.
    12. Aniza, Ria & Chen, Wei-Hsin & Lin, Yu-Ying & Tran, Khanh-Quang & Chang, Jo-Shu & Lam, Su Shiung & Park, Young-Kwon & Kwon, Eilhann E. & Tabatabaei, Meisam, 2021. "Independent parallel pyrolysis kinetics of extracted proteins and lipids as well as model carbohydrates in microalgae," Applied Energy, Elsevier, vol. 300(C).
    13. Gonzalez del Campo, A. & Lobato, J. & Cañizares, P. & Rodrigo, M.A. & Fernandez Morales, F.J., 2013. "Short-term effects of temperature and COD in a microbial fuel cell," Applied Energy, Elsevier, vol. 101(C), pages 213-217.
    14. Pang, Na & Gu, Xiangyu & Chen, Shulin & Kirchhoff, Helmut & Lei, Hanwu & Roje, Sanja, 2019. "Exploiting mixotrophy for improving productivities of biomass and co-products of microalgae," Renewable and Sustainable Energy Reviews, Elsevier, vol. 112(C), pages 450-460.
    15. Choi, Hong Il & Sung, Young Joon & Hong, Min Eui & Han, Jonghee & Min, Byoung Koun & Sim, Sang Jun, 2022. "Reconsidering the potential of direct microalgal biomass utilization as end-products: A review," Renewable and Sustainable Energy Reviews, Elsevier, vol. 155(C).
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    17. Samiee-Zafarghandi, Roudabeh & Karimi-Sabet, Javad & Abdoli, Mohammad Ali & Karbassi, Abdolreza, 2018. "Increasing microalgal carbohydrate content for hydrothermal gasification purposes," Renewable Energy, Elsevier, vol. 116(PA), pages 710-719.
    18. Juan Félix González & Teresa Belén Cuello & Antonio José Calderón & Manuel Calderón & Jerónimo González & Diego Carmona, 2021. "Cultivation of Autochthonous Microalgae for Biomass Feedstock: Growth Curves and Biomass Characterization for Their Use in Biorefinery Products," Energies, MDPI, vol. 14(15), pages 1-21, July.
    19. Suali, Emma & Sarbatly, Rosalam, 2012. "Conversion of microalgae to biofuel," Renewable and Sustainable Energy Reviews, Elsevier, vol. 16(6), pages 4316-4342.

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