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Use of natural substances for boar semen decontamination

Author

Listed:
  • J. Mazurova

    (Faculty of Chemical-Technology, University of Pardubice, Czech Republic)

  • R. Kukla

    (Faculty of Chemical-Technology, University of Pardubice, Czech Republic)

  • M. Rozkot

    (Institute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech Republic)

  • A. Lustykova

    (Institute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech Republic)

  • E. Slehova

    (Faculty of Chemical-Technology, University of Pardubice, Czech Republic)

  • R. Sleha

    (Faculty of Chemical-Technology, University of Pardubice, Czech Republic
    Faculty of Military Health Sciences, University of Defence, Hradec Kralove)

  • J. Lipensky

    (Institute of Animal Science, Prague-Uhrineves, Workplace Kostelec nad Orlici, Czech Republic)

  • L. Opletal

    (Faculty of Pharmacy, Hradec Kralove, Charles University, Prague, Czech Republic)

Abstract

The aim of this study was to investigate the antibacterial activity and toxicity for sperm cells of the natural substances gallic acid, methyl gallate, ethyl gallate, propyl gallate, octyl gallate, thymol, carvacrol and eugenol. The antibacterial activity of these natural substances and selected combinations of them against bacterial strains isolated from boar ejaculates was determined using the microdilution and macrodilution method in Mueller-Hinton broth. The most effective natural substances against Gram-negative and Gram-positive bacteria included in our study were thymol and carvacrol with minimum inhibitory concentration (MIC) values in the range of 300-600 µg/ml. Gallic acid exhibited the best antibacterial activity against Pseudomonas aeruginosa strains (MIC values of 300-2400 µg/ml), whereas the ranges of MIC values against Escherichia coli, Enterococcus faecalis and Staphylococcus sp. strains were higher. Octyl gallate exhibited stronger antibacterial activity against staphylococci and enterococci (MIC values of 18.8-75 µg/ml) than against Escherichia coli and Pseudomonas aeruginosa strains with MIC values in the ranges of 300-600 µg/mland 1200-2400 µg/ml, respectively. Thymol combined with carvacrol was the most effective combination against enterococci (MIC values of 75-300 : 150 thymol : carvacrol) and Pseudomonas aeruginosa (MIC values of 75-300 : 300 thymol : carvacrol), bacteria which are known to be frequently resistant to antimicrobials. Similar results were determined for the combination of carvacrol and eugenol against staphylococci and enterococci. The results of the combinations revealed more of an additive rather than a synergistic effect. Thymol and carvacrol were the most effective natural substances against the bacteria included in this study, with a low toxicity for sperm cells compared to other substances, suggesting their possible use for boar semen decontamination.

Suggested Citation

  • J. Mazurova & R. Kukla & M. Rozkot & A. Lustykova & E. Slehova & R. Sleha & J. Lipensky & L. Opletal, 2015. "Use of natural substances for boar semen decontamination," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 60(5), pages 235-247.
    • Handle: RePEc:caa:jnlvet:v:60:y:2015:i:5:id:8175-vetmed
    DOI: 10.17221/8175-VETMED
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    References listed on IDEAS

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    1. H. Vondruskova & R. Slamova & M. Trckova & Z. Zraly & I. Pavlik, 2010. "Alternatives to antibiotic growth promoters in prevention of diarrhoea in weaned piglets: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(5), pages 199-224.
    2. F. Sisak & H. Havlickova & H. Hradecka & I. Rychlik & I. Kolackova & R. Karpiskova, 2006. "Antibiotic resistance of Salmonella spp. isolates from pigs in the Czech Republic," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 303-310.
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