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Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report

Author

Listed:
  • M. Blahutkova

    (Veterinary Research Institute, Brno, Czech Republic)

  • P. Fictum

    (University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • M. Skoric

    (University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • B. Bezdekova

    (University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • P. Jahn

    (University of Veterinary and Pharmaceutical Sciences Brno, Czech Republic)

  • P. Kriz

    (Veterinary Research Institute, Brno, Czech Republic)

  • V. Mrlik

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Slana

    (Veterinary Research Institute, Brno, Czech Republic)

  • M. Kaevska

    (Veterinary Research Institute, Brno, Czech Republic)

  • I. Pavlik

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

This report describes new possibilities for intravital and post mortem diagnosis of avian mycobacteriosis in horses using the quantitative real time PCR (qPCR) method. Using this method, Mycobacterium avium subsp. hominissuis was diagnosed in two sibling Fjord horses. In the first horse, M. a. hominissuis was detected by qPCR in numbers of 2.89 × 105 and 1.47 × 104 cells per 1 g of intestinal content and mesenteric lymph nodes, respectively; in the second horse, faeces and mesenteric lymph node samples showed numbers of 6.31 × 105 and 3.36 × 106 cells per 1 g of tissue, respectively. Another aim of this study was to comprehensively describe clinical and pathological findings in both animals.

Suggested Citation

  • M. Blahutkova & P. Fictum & M. Skoric & B. Bezdekova & P. Jahn & P. Kriz & V. Mrlik & I. Slana & M. Kaevska & I. Pavlik, 2011. "Mycobacterium avium subsp. hominissuis infection in two sibling Fjord horses diagnosed using quantitative real time PCR: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 56(6), pages 294-301.
    • Handle: RePEc:caa:jnlvet:v:56:y:2011:i:6:id:1544-vetmed
    DOI: 10.17221/1544-VETMED
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    References listed on IDEAS

    as
    1. M. Moravkova & I. Trcka & J. Lamka & I. Pavlik, 2008. "A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(8), pages 445-451.
    2. M. Pate & M. Moravkova & B. Krt & I. Pavlik & M. Ocepek, 2009. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 270-279.
    3. I. Pavlik & P. Jahn & L. Dvorska & M. Bartos & L. Novotny & R. Halouzka, 2004. "Mycobacterial infections in horses: a review of the literature," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 49(11), pages 427-440.
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