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Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages

Author

Listed:
  • Petr Králík

    (Veterinary Research Institute, Brno, Czech Republic)

  • Hana Přikrylová Vondrušková

    (Veterinary Research Institute, Brno, Czech Republic)

  • Iva Slaná

    (Veterinary Research Institute, Brno, Czech Republic)

  • Monika Morávková

    (Veterinary Research Institute, Brno, Czech Republic)

  • Ivo Pavlík

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

We assessed the survival of Mycobacterium avium subsp. hominissuis (MAH) in artificially contaminated homemade smoked sausages prepared from pork meat according to traditional recipes, the effect of storage of such sausages at -20°C for three months on MAH viability and to compare assessment of MAH viability/presence by culture and qPCR. Three isolates of MAH were inoculated into the sausage mixture at concentrations of 106 CFU per gram of meat and cold smoked at 40°C for 12 h or hot smoked at 70°C for 6 hours. MAH survived the cold smoking procedure without any significant decrease in viable MAH CFU counts; no viable MAH were detected in the hot smoked sausages. The storage of sausages at -20°C caused a decrease in viable MAH counts of about 1 to 3 log10. Absolute MAH counts determined by qPCR were not significantly reduced by the storage or smoking. The presence of viable MAH in sausages after the cold smoking should be considered as a risk for immunodeficient individuals and children.

Suggested Citation

  • Petr Králík & Hana Přikrylová Vondrušková & Iva Slaná & Monika Morávková & Ivo Pavlík, 2011. "Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(6), pages 647-653.
    • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:6:id:444-2010-cjfs
    DOI: 10.17221/444/2010-CJFS
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    References listed on IDEAS

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    1. M. Kaevska & I. Slana & P. Kralik & I. Pavlik, 2010. "Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(7), pages 325-330.
    2. I. Trcka & J. Lamka & R. Suchy & M. Kopecna & V. Beran & M. Moravkova & A. Horvathova & M. Bartos & I. Parmova & I. Pavlik, 2006. "Mycobacterial infections in European wild boar (Sus scrofa) in the Czech Republic during the years 2002 to 2005," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 320-332.
    3. I. Pavlik & L. Matlova & L. Dvorska & J.E. Shitaye & I. Parmova, 2005. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 281-290.
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