IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjf/v29y2011i6id444-2010-cjfs.html
   My bibliography  Save this article

Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages

Author

Listed:
  • Petr Králík

    (Veterinary Research Institute, Brno, Czech Republic)

  • Hana Přikrylová Vondrušková

    (Veterinary Research Institute, Brno, Czech Republic)

  • Iva Slaná

    (Veterinary Research Institute, Brno, Czech Republic)

  • Monika Morávková

    (Veterinary Research Institute, Brno, Czech Republic)

  • Ivo Pavlík

    (Veterinary Research Institute, Brno, Czech Republic)

Abstract

We assessed the survival of Mycobacterium avium subsp. hominissuis (MAH) in artificially contaminated homemade smoked sausages prepared from pork meat according to traditional recipes, the effect of storage of such sausages at -20°C for three months on MAH viability and to compare assessment of MAH viability/presence by culture and qPCR. Three isolates of MAH were inoculated into the sausage mixture at concentrations of 106 CFU per gram of meat and cold smoked at 40°C for 12 h or hot smoked at 70°C for 6 hours. MAH survived the cold smoking procedure without any significant decrease in viable MAH CFU counts; no viable MAH were detected in the hot smoked sausages. The storage of sausages at -20°C caused a decrease in viable MAH counts of about 1 to 3 log10. Absolute MAH counts determined by qPCR were not significantly reduced by the storage or smoking. The presence of viable MAH in sausages after the cold smoking should be considered as a risk for immunodeficient individuals and children.

Suggested Citation

  • Petr Králík & Hana Přikrylová Vondrušková & Iva Slaná & Monika Morávková & Ivo Pavlík, 2011. "Survival of Mycobacterium avium subsp. hominissuis in homemade smoked pork sausages," Czech Journal of Food Sciences, Czech Academy of Agricultural Sciences, vol. 29(6), pages 647-653.
    • Handle: RePEc:caa:jnlcjf:v:29:y:2011:i:6:id:444-2010-cjfs
    DOI: 10.17221/444/2010-CJFS
    as

    Download full text from publisher

    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/444/2010-CJFS.html
    Download Restriction: free of charge
    File URL: http://cjfs.agriculturejournals.cz/doi/10.17221/444/2010-CJFS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/444/2010-CJFS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. M. Kaevska & I. Slana & P. Kralik & I. Pavlik, 2010. "Examination of Mycobacterium avium subsp. avium distribution in naturally infected hens by culture and triplex quantitative real time PCR," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 55(7), pages 325-330.
    2. I. Pavlik & L. Matlova & L. Dvorska & J.E. Shitaye & I. Parmova, 2005. "Mycobacterial infections in cattle and pigs caused by Mycobacterium aviumcomplex members and atypical mycobacteria in theCzech Republicduring 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 281-290.
    3. I. Trcka & J. Lamka & R. Suchy & M. Kopecna & V. Beran & M. Moravkova & A. Horvathova & M. Bartos & I. Parmova & I. Pavlik, 2006. "Mycobacterial infections in European wild boar (Sus scrofa) in the Czech Republic during the years 2002 to 2005," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 51(5), pages 320-332.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. M. Pate & M. Moravkova & B. Krt & I. Pavlik & M. Ocepek, 2009. "Genotyping of Mycobacterium avium subsp. avium isolates from domestic animals in Slovenia by IS901 RFLP," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 54(6), pages 270-279.
    2. I. Pavlik & I. Trcka & I. Parmova & J. Svobodova & I. Melicharek & G. Nagy & Z. Cvetnic & M. Ocepek & M. Pate & M. Lipiec, 2005. "Detection of bovine and human tuberculosis in cattle and other animals in six Central European countries during the years 2000-2004," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 50(7), pages 291-299.
    3. M. Moravkova & I. Trcka & J. Lamka & I. Pavlik, 2008. "A mixed infection of Mycobacterium avium subsp. paratuberculosis and M. a. hominissuis in one red deer (Cervus elaphus) studied by IS900 BstEII and IS1245 PvuII RFLP analyses: a case report," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 53(8), pages 445-451.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjf:v:29:y:2011:i:6:id:444-2010-cjfs. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.