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Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols

Author

Listed:
  • L. Růžek

    (Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • M. Růžková

    (Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic
    Central Institute for Supervising and Testing in Agriculture, Brno, Czech Republic)

  • K. Voříšek

    (Faculty of Agrobiology, Food and Natural Resources, Czech University of Life Sciences, Prague, Czech Republic)

  • J. Kubát

    (Crop Research Institute, Prague, Czech Republic)

  • M. Friedlová

    (Crop Research Institute, Prague, Czech Republic)

  • O. Mikanová

    (Crop Research Institute, Prague, Czech Republic)

Abstract

Cambisols, Luvisols and Stagnosols are main soil taxonomical units covering 78% of the total area of the Czech Republic. In the period 2001-2008 soil samples from 13 arable and 2 grassed localities were collected and tested. Microbial biomass carbon (MBC) was determined using microwave irradiation method (MW), chloroform fumigation extraction method (FE) and re-hydration technique (RHD). Soil samples were moistened, according to the content of organic carbon (Corg), by deionized water addition (%Corg × 0.19 ml/g DM - dry mass of soil) immediately before MBC determination. Microwave sterilization (800 J/g DM = 600 W, 2 × 67 s, 100 g DM (10 soil samples) and microwave soil extracts digestion (800 J/ml = 250 W, 77 s, 24 ml) give the lowest values of MBC (204 ± 67 mg/kg DM; 100%) in comparison with FE (236 ± 57 mg/kg DM; 116%) and RHD (478 ± 138 mg/kg DM; 235%), respectively. High significant correlation (r = 0.9713) was found between TC (total carbon; 1.36 ± 0.29%) and TN (total nitrogen; 0.15 ± 0.03%) determined by CNS analyzer. Furthermore, high significant correlations were found between MBC-MW and MBC-RHD (r = 0.8965) as well as MBC-FE and DHA (dehydrogenase activity; r = 0.8094), respectively. DHA in studied soils reached 147 ± 68 mg of triphenylformazan/kg DM/24 h. Corg formed 96% of TC and total Kjeldahl nitrogen 97% of TN, respectively. According our results MW is fully acceptable for MBC determination.

Suggested Citation

  • L. Růžek & M. Růžková & K. Voříšek & J. Kubát & M. Friedlová & O. Mikanová, 2009. "Chemical and microbiological characterization of Cambisols, Luvisols and Stagnosols," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 55(6), pages 231-237.
    • Handle: RePEc:caa:jnlpse:v:55:y:2009:i:6:id:35-2009-pse
    DOI: 10.17221/35/2009-PSE
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    References listed on IDEAS

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    1. O. Mikanová & M. Javůrek & M. Vach & A. Markupová, 2006. "The influence of tillage on selected biological parameters," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 52(6), pages 271-274.
    2. O. Mikanová & M. Friedlová & T. Šimon, 2009. "The influence of fertilisation and crop rotation on soil microbial characteristics in the long-term field experiment," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 55(1), pages 11-16.
    3. T. Šimon, 2005. "Aliphatic compounds, organic C and N and microbial biomass and its activity in long-term field experiment," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 51(6), pages 276-282.
    4. J. Haberle & P. Svoboda & I. Raimanová, 2008. "The effect of post-anthesis water supply on grain nitrogen concentration and grain nitrogen Šeld of winter wheat," Plant, Soil and Environment, Czech Academy of Agricultural Sciences, vol. 54(7), pages 304-312.
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