IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v67y2022i11id103-2022-cjas.html
   My bibliography  Save this article

Differentially expressed genes in the longissimus dorsi muscle between the Chinese indigenous Ningxiang pig and Large White breed using RNA sequencing

Author

Listed:
  • Fang Wang

    (Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China)

  • Linfeng Lei

    (Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China
    Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, P.R. China)

  • Zhaobin Wang

    (Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, P.R. China)

  • Yulong Yin

    (Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China)

  • Huansheng Yang

    (Hunan International Joint Laboratory of Animal Intestinal Ecology and Health, Laboratory of Animal Nutrition and Human Health, College of Life Sciences, Hunan Normal University, Changsha, P.R. China)

  • Zhe Yang

    (Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China)

  • Jiashun Chen

    (Animal Nutritional Genome and Germplasm Innovation Research Center, College of Animal Science and Technology, Hunan Agricultural University, Changsha, P.R. China)

Abstract

High intramuscular fat content of pigs improves pork quality and increasing intramuscular fat deposition is a long-term goal in the husbandry of pigs reared for meat production. There are significant phenotypic differences between the Ningxiang (NX) pigs (an indigenous Chinese breed) and Large White (LW) pigs (a western, lean-type breed). The present work aimed to gain insight into the longissimus dorsi muscle transcriptome between the two pig breeds. We investigated the molecular basis of these differences by comparing their transcriptome profiles. RNA-seq technology was used to identify the differentially expressed genes (DEGs) in the longissimus dorsi muscle of the NX and LW pigs. We obtained 692 million clean reads using transcriptome sequencing of muscle samples from the two pig breeds. A total of 885 DEGs were identified, including 469 upregulated and 416 downregulated genes in the NX pigs compared with the LW pigs. Using KEGG pathway analysis, it was found that the significant DEGs were mainly enriched in metabolism-related pathways, such as lipid metabolism and biosynthesis, and glucose metabolism or biosynthesis. Quantitative real-time PCR confirmed the differential expression of eight selected DEGs in both pig breeds. qPCR results showed that the RNA-seq results were reliable. Several DEGs were candidate functional genes related to the lipid metabolism, including CD36, LIPE, MCAT, LPIN1, ANGPTL4, PPARD, SCD, INSR, MOGAT, IGF1, AKT2 and JAK2. Our results provide a comprehensive basis for the investigation of the differences in transcriptional regulation of the muscles between divergent phenotypes.

Suggested Citation

  • Fang Wang & Linfeng Lei & Zhaobin Wang & Yulong Yin & Huansheng Yang & Zhe Yang & Jiashun Chen, 2022. "Differentially expressed genes in the longissimus dorsi muscle between the Chinese indigenous Ningxiang pig and Large White breed using RNA sequencing," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 67(11), pages 442-453.
    • Handle: RePEc:caa:jnlcjs:v:67:y:2022:i:11:id:103-2022-cjas
    DOI: 10.17221/103/2022-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/103/2022-CJAS.html
    Download Restriction: free of charge
    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/103/2022-CJAS.pdf
    Download Restriction: free of charge
    File URL: https://libkey.io/10.17221/103/2022-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Alan R. Saltiel & C. Ronald Kahn, 2001. "Insulin signalling and the regulation of glucose and lipid metabolism," Nature, Nature, vol. 414(6865), pages 799-806, December.
    Full references (including those not matched with items on IDEAS)

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Julian Krauskopf & Theo M de Kok & Shelli J Schomaker & Mark Gosink & Deborah A Burt & Patricia Chandler & Roscoe L Warner & Kent J Johnson & Florian Caiment & Jos C Kleinjans & Jiri Aubrecht, 2017. "Serum microRNA signatures as "liquid biopsies" for interrogating hepatotoxic mechanisms and liver pathogenesis in human," PLOS ONE, Public Library of Science, vol. 12(5), pages 1-17, May.
    2. Cemal Erdem & Sean M. Gross & Laura M. Heiser & Marc R. Birtwistle, 2023. "MOBILE pipeline enables identification of context-specific networks and regulatory mechanisms," Nature Communications, Nature, vol. 14(1), pages 1-16, December.
    3. Sok Kuan Wong & Kok-Yong Chin & Farihah Hj Suhaimi & Fairus Ahmad & Soelaiman Ima-Nirwana, 2018. "The Effects of Vitamin E from Elaeis guineensis (Oil Palm) in a Rat Model of Bone Loss Due to Metabolic Syndrome," IJERPH, MDPI, vol. 15(9), pages 1-12, August.
    4. Yafei Yuan & Fang Kong & Hanwen Xu & Angqi Zhu & Nieng Yan & Chuangye Yan, 2022. "Cryo-EM structure of human glucose transporter GLUT4," Nature Communications, Nature, vol. 13(1), pages 1-8, December.
    5. Yan-Ping Zhang & Wen-Hong Zhang & Pan Zhang & Qi Li & Yue Sun & Jia-Wen Wang & Shaobing O. Zhang & Tao Cai & Cheng Zhan & Meng-Qiu Dong, 2022. "Intestine-specific removal of DAF-2 nearly doubles lifespan in Caenorhabditis elegans with little fitness cost," Nature Communications, Nature, vol. 13(1), pages 1-18, December.
    6. Eunyoung Kim & Jiamei Cui & Inhae Kang & Guiguo Zhang & Yunkyoung Lee, 2021. "Potential Antidiabetic Effects of Seaweed Extracts by Upregulating Glucose Utilization and Alleviating Inflammation in C2C12 Myotubes," IJERPH, MDPI, vol. 18(3), pages 1-13, February.
    7. Hui Xia & Charlotte Scholtes & Catherine R. Dufour & Carlo Ouellet & Majid Ghahremani & Vincent Giguère, 2022. "Insulin action and resistance are dependent on a GSK3β-FBXW7-ERRα transcriptional axis," Nature Communications, Nature, vol. 13(1), pages 1-19, December.
    8. Gunnar Cedersund & Jacob Roll & Erik Ulfhielm & Anna Danielsson & Henrik Tidefelt & Peter Strålfors, 2008. "Model-Based Hypothesis Testing of Key Mechanisms in Initial Phase of Insulin Signaling," PLOS Computational Biology, Public Library of Science, vol. 4(6), pages 1-10, June.
    9. Nicholas S. Kirk & Qi Chen & Yingzhe Ginger Wu & Anastasia L. Asante & Haitao Hu & Juan F. Espinosa & Francisco Martínez-Olid & Mai B. Margetts & Faiz A. Mohammed & Vladislav V. Kiselyov & David G. Ba, 2022. "Activation of the human insulin receptor by non-insulin-related peptides," Nature Communications, Nature, vol. 13(1), pages 1-13, December.
    10. Mohd Zamri Bin Haji Ismail & Matt D Hodges & Michael Boylan & Rajesh Achall & Alan Shirras & Susan J Broughton, 2015. "The Drosophila Insulin Receptor Independently Modulates Lifespan and Locomotor Senescence," PLOS ONE, Public Library of Science, vol. 10(5), pages 1-21, May.
    11. Kaoru Ohashi & Hisako Komada & Shinsuke Uda & Hiroyuki Kubota & Toshinao Iwaki & Hiroki Fukuzawa & Yasunori Komori & Masashi Fujii & Yu Toyoshima & Kazuhiko Sakaguchi & Wataru Ogawa & Shinya Kuroda, 2015. "Glucose Homeostatic Law: Insulin Clearance Predicts the Progression of Glucose Intolerance in Humans," PLOS ONE, Public Library of Science, vol. 10(12), pages 1-19, December.
    12. Lei Ying & Luyao Wang & Kaiwen Guo & Yushu Hou & Na Li & Shuyi Wang & Xingfeng Liu & Qijin Zhao & Jie Zhou & Longwei Zhao & Jianlou Niu & Chuchu Chen & Lintao Song & Shaocong Hou & Lijuan Kong & Xiaok, 2021. "Paracrine FGFs target skeletal muscle to exert potent anti-hyperglycemic effects," Nature Communications, Nature, vol. 12(1), pages 1-14, December.
    13. Katarina Baralić & Aleksandra Buha Djordjevic & Katarina Živančević & Evica Antonijević & Milena Anđelković & Dragana Javorac & Marijana Ćurčić & Zorica Bulat & Biljana Antonijević & Danijela Đukić-Ćo, 2020. "Toxic Effects of the Mixture of Phthalates and Bisphenol A—Subacute Oral Toxicity Study in Wistar Rats," IJERPH, MDPI, vol. 17(3), pages 1-24, January.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:67:y:2022:i:11:id:103-2022-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.