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Genome reprogramming during the first cell cycle in in vitro produced porcine embryos

Author

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  • I. Barnetová

    (Institute of Animal Science, Prague, Czech Republic
    Center for Cell Therapy and Tissue Repair, IAPG CAS, Liběchov, Czech Republic)

  • K. Okada

    (Nippon Veterinary and Life Science University, Musashino, Tokyo, Japan)

Abstract

Conflicting data still exist regarding the extent of paternal pronuclear DNA demethylation in one cell-stage mammalian embryos. Demethylation of paternal pronuclear DNA was observed in in vitro produced porcine zygotes, whereas in vitro produced embryos do not show any or only weak paternal genome demethylation. In our experiments, we have used and compared two in vitro techniques commonly used for in vitro embryo production (in vitro fertilization and intracytoplasmic sperm injection) and then we evaluated the extent of labelling in both these groups after 5-methylcytosine (5-MeC) or dimethyl H3/K9 labelling. We have found no differences in the methylation pattern between both those techniques used for the production of embryos. Moreover, we did not detect any demethylation of paternal DNA after 5-MeC labelling at all. Contrary to this, labelling with dimethyl H3/K9 antibodies showed differences in labelling intensity between maternal and paternal genomes in 42% of zygotes after in vitro fertilization and in 44% of zygotes after intracytoplasmic sperm injection. Our results indicate that in vitro matured pig oocytes exhibit rather inconsistent methylation patterns. This inconsistency probably resulted from insufficient cytoplasmic maturation of oocytes and to a lesser extent from the in vitro technique for embryo production.

Suggested Citation

  • I. Barnetová & K. Okada, 2010. "Genome reprogramming during the first cell cycle in in vitro produced porcine embryos," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 55(2), pages 49-57.
    • Handle: RePEc:caa:jnlcjs:v:55:y:2010:i:2:id:26-2009-cjas
    DOI: 10.17221/26/2009-CJAS
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    References listed on IDEAS

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    1. Z.G. Wang & Z.R. XU & S.D. YU, 2007. "Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(1), pages 21-25.
    2. Brian D. Strahl & C. David Allis, 2000. "The language of covalent histone modifications," Nature, Nature, vol. 403(6765), pages 41-45, January.
    3. Wolfgang Mayer & Alain Niveleau & Jörn Walter & Reinald Fundele & Thomas Haaf, 2000. "Demethylation of the zygotic paternal genome," Nature, Nature, vol. 403(6769), pages 501-502, February.
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    Cited by:

    1. I. Barnetova & I. Vackova & P. Firla, 2012. "Dynamics of epigenetic remodeling in interspecies porcine zygotes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 57(2), pages 83-94.

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