IDEAS home Printed from https://ideas.repec.org/a/caa/jnlcjs/v50y2005i7id4171-cjas.html
   My bibliography  Save this article

Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)

Author

Listed:
  • I. Petrová

    (Grammar School, Říčany near Prague, Czech Republic)

  • R. Rajmon

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic)

  • M. Sedmíková

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic)

  • Z. Kuthanová

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic)

  • F. Jílek

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic)

  • J. Rozinek

    (Department of Veterinary Sciences, Faculty of Agrobiology, Food and Natural Resources, Czech University of Agriculture, Prague, Czech Republic)

Abstract

There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many possible ways of meeting this demand. However, oocytes further cultured in vitro after reaching the stage of metaphase II undergo the complex process called "ageing", which decreases the quality of oocytes. The aim of the present study was to improve the culture conditions for in vitro matured porcine oocytes by supplement of the epidermal growth factor (EGF) or the insulin-like growth factor 1 (IGF-1) in order to reduce the adverse effects of ageing. Supplement of either EGF or IGF-1 had no significant effect on the ageing of porcine oocytes. Significant effects were demonstrated when the oocytes were cultured with both EGF and IGF-1. A combination of 10 µg EGF/ml with 25 ng IGF-1/ml was the most effective. Oocytes aged under these conditions retained very good developmental competence. We observed development to the morula (21%) or blastocyst (25%) stage in oocytes aged for 1 day with EGF and IGF-1. Porcine oocytes matured in vitro are more resistant to the ageing when cultured in the presence of both EGF and IGF-1 and these conditions retain an elevated developmental competence for a certain time.

Suggested Citation

  • I. Petrová & R. Rajmon & M. Sedmíková & Z. Kuthanová & F. Jílek & J. Rozinek, 2005. "Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 50(7), pages 300-310.
  • Handle: RePEc:caa:jnlcjs:v:50:y:2005:i:7:id:4171-cjas
    DOI: 10.17221/4171-CJAS
    as

    Download full text from publisher

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4171-CJAS.html
    Download Restriction: free of charge

    File URL: http://cjas.agriculturejournals.cz/doi/10.17221/4171-CJAS.pdf
    Download Restriction: free of charge

    File URL: https://libkey.io/10.17221/4171-CJAS?utm_source=ideas
    LibKey link: if access is restricted and if your library uses this service, LibKey will redirect you to where you can use your library subscription to access this item
    ---><---

    As the access to this document is restricted, you may want to search for a different version of it.

    References listed on IDEAS

    as
    1. Joshua Johnson & Jacqueline Canning & Tomoko Kaneko & James K. Pru & Jonathan L. Tilly, 2004. "Correction: Corrigendum: Germline stem cells and follicular renewal in the postnatal mammalian ovary," Nature, Nature, vol. 430(7003), pages 1062-1062, August.
    2. I. Petrová & M. Sedmíková & E. Chmelíková & D. Švestková & R. Rajmon, 2004. "In vitro aging of porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 49(3), pages 93-98.
    3. Joshua Johnson & Jacqueline Canning & Tomoko Kaneko & James K. Pru & Jonathan L. Tilly, 2004. "Germline stem cells and follicular renewal in the postnatal mammalian ovary," Nature, Nature, vol. 428(6979), pages 145-150, March.
    Full references (including those not matched with items on IDEAS)

    Citations

    Citations are extracted by the CitEc Project, subscribe to its RSS feed for this item.
    as


    Cited by:

    1. J. Petr & E. Chmelíková & L. Tůmová & M. Ješeta, 2007. "The role of nitric oxide in parthenogenetic activation of pig oocytes: A review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(11), pages 363-377.
    2. J. Nevoral & T. Krejčová & J. Petr & P. Melicharová & A. Vyskočilová & M. Dvořáková & I. Weingartová & E. Chmelíková & L. Tůmová & K. Hošková & V. Kučerová-Chrpová & M. Sedmíková, 2013. "The role of nitric oxide synthase isoforms in aged porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(10), pages 453-459.
    3. Z. Vodková & R. Rajmon & J. Petr & P. Klabanová & F. Jílek, 2008. "Effects of genistein and genistin on in vitro maturation of pig oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 53(1), pages 1-8.
    4. J. Petr & E. Chmelíková & A. Dörflerová & M. Ješeta & Z. Kuthanová, 2007. "Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(12), pages 415-422.

    Most related items

    These are the items that most often cite the same works as this one and are cited by the same works as this one.
    1. Rita Canipari & Lucia De Santis & Sandra Cecconi, 2020. "Female Fertility and Environmental Pollution," IJERPH, MDPI, vol. 17(23), pages 1-18, November.
    2. A. Danylevska & J. Sebestova, 2013. "Causes and consequences of maternal age-related aneuploidy in oocytes: a review," Veterinární medicína, Czech Academy of Agricultural Sciences, vol. 58(2), pages 65-72.
    3. J. Petr & E. Chmelíková & L. Tůmová & M. Ješeta, 2007. "The role of nitric oxide in parthenogenetic activation of pig oocytes: A review," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(11), pages 363-377.
    4. M. Sedmíková & J. Petr & A. Dörflerová & J. Nevoral & B. Novotná & T. Krejčová & E. Chmelíková & L. Tůmová, 2013. "Inhibition of c-Jun N-terminal kinase (JNK) suppresses porcine oocyte ageing in vitro," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(12), pages 535-545.
    5. J. Petr & E. Chmelíková & A. Dörflerová & M. Ješeta & Z. Kuthanová, 2007. "Effects of protein kinase C on parthenogenetic activation of pig oocytes using calcium ionophore or nitric oxide-donor," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 52(12), pages 415-422.
    6. J. Nevoral & T. Krejčová & J. Petr & P. Melicharová & A. Vyskočilová & M. Dvořáková & I. Weingartová & E. Chmelíková & L. Tůmová & K. Hošková & V. Kučerová-Chrpová & M. Sedmíková, 2013. "The role of nitric oxide synthase isoforms in aged porcine oocytes," Czech Journal of Animal Science, Czech Academy of Agricultural Sciences, vol. 58(10), pages 453-459.
    7. W Hamish B Wallace & Thomas W Kelsey, 2010. "Human Ovarian Reserve from Conception to the Menopause," PLOS ONE, Public Library of Science, vol. 5(1), pages 1-9, January.

    Corrections

    All material on this site has been provided by the respective publishers and authors. You can help correct errors and omissions. When requesting a correction, please mention this item's handle: RePEc:caa:jnlcjs:v:50:y:2005:i:7:id:4171-cjas. See general information about how to correct material in RePEc.

    If you have authored this item and are not yet registered with RePEc, we encourage you to do it here. This allows to link your profile to this item. It also allows you to accept potential citations to this item that we are uncertain about.

    If CitEc recognized a bibliographic reference but did not link an item in RePEc to it, you can help with this form .

    If you know of missing items citing this one, you can help us creating those links by adding the relevant references in the same way as above, for each refering item. If you are a registered author of this item, you may also want to check the "citations" tab in your RePEc Author Service profile, as there may be some citations waiting for confirmation.

    For technical questions regarding this item, or to correct its authors, title, abstract, bibliographic or download information, contact: Ivo Andrle (email available below). General contact details of provider: https://www.cazv.cz/en/home/ .

    Please note that corrections may take a couple of weeks to filter through the various RePEc services.

    IDEAS is a RePEc service. RePEc uses bibliographic data supplied by the respective publishers.